Protective effects of quercetin on testicular torsion/detorsion-induced ischaemia-reperfusion injury in rats

Abstract

The aim of this study was to investigate the protective effect of quercetin (QE) on testicular torsion/detorsion-induced ischaemia-reperfusion (I/R) injury. A total of 24 male Wistar albino rats were divided into three groups: control, I/R and I/R treated with QE; each group contain eight animals. Testicular torsion was created by rotating the left testis 720° in a clockwise direction. The ischaemia period was 5 h and orchiectomy was performed after 5 h of detorsion. QE (15 mg kg(-1) , i.p.) was administered only once, 40 min prior to detorsion. Left orchiectomy was performed in all I/R groups. To date, no histopathological changes on testicular torsion/detorsion-induced I/R injury in rats by QE treatment have been reported. Spermatogenesis and mean seminiferous tubule diameter were significantly decreased in I/R groups were compared with the control group. Furthermore, QE treated animals showed an improved histological appearance in I/R group. Our data indicate a significant reduction in the activity of TUNEL, endothelial nitric oxide synthase and a rise in the expression of testosterone in testes tissue of I/R treated with QE therapy. We believe that further preclinical research into the utility of QE may indicate its usefulness as a potential treatment on testes injury after I/R in rats.

Protective effects ofStevia rebaudianaaqueous extract on experimental unilateral testicular ischaemia/reperfusion injury in rats

“…These changes of MDA, GPX and SOD levels in T/D group were also observed in similar studies that treatment with some antioxidant substances like verapamil, onion juice, Matricaria chamomilla, thymoquinone and quercetin could improve this biochemical condition to approximately healthy condition (Aktoz, Kanter, & Aktas, 2010;Ameli et al, 2018;Ayan et al, 2016;Shokoohi, Madarek, et al, 2018;Soltani et al, 2018). There are two major limitations in this study.…”

Protective effects of thymoquinone on experimental testicular ischaemia-reperfusion injury: an apoptotic, proliferative and biochemical study

“…ROS cause DNA damage, lipid peroxidation, expression of cytokines, histological inflammation and, eventually, fibrogenesis, apoptosis or cell death with necrosis by affecting many reactions in cell metabolism (Lavine, 2000;Chatterjee et al, 2002). Aktoz et al (2010) and Kanter (2011a) found that the application of I/R caused apoptosis in testes. In our study, it was found that I/R increase the number of apoptotic cells and the apoptotic index in the testis.…”

Comparison of quercetin and resveratrol in the prevention of injury due to testicular torsion/detorsion in rats

“…Several plant extracts have been studied in animal models for their potential as secondary treatments to surgical repair of testicular torsion. Aktoz et al 11. demonstrated that I/R of 5 h causes obvious injury to the testis, and the administration of QE improved the histopathological parameters, increased the expression of testicular eNOS, and increased germ cell apoptosis in the affected testis.…”

“…According to previous studies, quercetin (QE) is a powerful antioxidant agent with the ability to reduce tissue damage after I/R processes 891011. Resveratrol (RSV) is found in many plants, particularly grapes and peanuts.…”