Comparison of quercetin and resveratrol in the prevention of injury due to testicular torsion/detorsion in rats

Abstract

Quercetin (QE) and resveratrol (RSV) are powerful antioxidants with the potential to protect the testes against ischemia/reperfusion (I/R) injury. We compared their effects in testicular torsion/detorsion (T/D) in adult rats. Twenty-four male Wistar rats were divided into four groups: sham (group A), T/D (group B), T/D treated with QE (group C), and T/D treated with RSV (group D). QE (20 mg kg−1) and RSV (20 mg kg−1) were injected intra-peritoneally at 60 min of torsion. After 90 min of surgically induced torsion, the testicular cord was restored to its anatomical position. Twenty-four hour after torsion, blood and tissue samples were obtained for further examination. Testicular tissue malondialdehyde (MDA) and nitric oxide (NO) levels and serum total oxidant status (TOS) were higher in group B than in group A (P < 0.05). Group A had higher serum total antioxidant status (TAS) than group B. (P < 0.05) QE and RSV significantly lowered MDA, NO, and TOS levels and TAS consumption (P < 0.05). QE reduced the MDA and TOS levels more than RSV (P < 0.05), but their effects on NO reduction and TAS consumption were similar (P > 0.05). Group A had normal testicular architecture (grade 1). Groups C (mean grade 2.60) and D (mean grade 3.00) had lower testicular injury grades than group B (mean grade 3.45) (P < 0.05). Group C had lower testicular injury grade than group D (P < 0.05). Treatment with QE and RSV protects against I/R injury after testicular T/D. QE may exhibit better function than RSV at the doses tested in this study.

Quercetin ameliorates cytotoxic effects of zinc oxide nanoparticles on sertoli cells by enhancing autophagy and suppressing oxidative stress

“…Chi et al. (2016) have shown preventive impacts of QCT and resveratrol against testicular torsion/detorsion through enhancing antioxidant levels and reducing lipid peroxidation in rats. Yelumalai et al.…”

Effect of etoricoxib on experimental oxidative testicular ischemia-reperfusion damage in rats induced with torsion-detorsion

“…However, dilated congested blood vessels in the interstitium, mild disorganisation in the seminiferous tubules, decreased spermatid and edema were found in the ETD-50 group, which suppressed oxidants and cytokines milder than those in the ETD-100 group. Torsion-detorsion has been experimentally demonstrated to lead to pathological changes, including disorganisation in the seminiferous tubules, necrosis of the germinal cells, decreased spermatozoa [ 39 ] and interstitial edema [ 4 ]. In their study, Minutoli et al histopathologically showed severe damage in the testes caused by I/R [ 40 ].…”

MALAT1 Promotes Cell Apoptosis and Suppresses Cell Proliferation in Testicular Ischemia-Reperfusion Injury by Sponging MiR-214 to Modulate TRPV4 Expression

“…The pathophysiological mechanisms of testicular T/D center around testicular IRI, in which torsion results in ischemic damage and detorsion leads to enhanced blood flow and subsequent reperfusion injury, resulting in spermatogenic cell apoptosis and the disruption of spermatogenesis, eventually leading to testicular dysfunction and infertility [23, 24]. The two major factors affecting testicular damage are the duration and degree of spermatic cord torsion, with testicular salvage rates from manipulative and operative reduction ranging from 42% to 88% [25, 26].…”