Protective effects of propolis on cryopreservation of common carp (Cyprinus carpio) sperm

Öğretmen, Fatih; İnanan, Burak Evren

doi:10.1016/j.cryobiol.2014.01.003

Cited by 29 publications

(16 citation statements)

References 46 publications

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“…Antioxidant molecules, such as ascorbic acid (vitamin C), catalase, glutathione and cysteine, may reduce the impact of oxidative stress and thus improve quality of thawed sperm, which have been tested in vitro and in vivo, on human, bovine, ram, rabbit, horse and boar sperm (Chatterjee & Gagnon, ; Großfeld et al., ; Maxwell & Stojanov, ; Vernet, Aitken, & Drevet, ) with inconsistent results. Available data on fish are scarce (Lahnsteiner, Mansour, & Kunz, ; Martinez‐Paramoa et al., ; Ogretmen, Inanan, & Ozturk, ; Ubilla & Valdebenito, ). Our previous work demonstrated that cryopreservation resulted in significant osmotic and oxidative stress in common carp Cyprinus carpio spermatozoa (Li & Li et al., ).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species

et al. 2017

Reprod Domestic Animals

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High levels of reactive oxygen species (ROS), which may be associated with reduced sperm quality, can be detected during cryopreservation of sperm of some species. Our objective was to investigate whether the addition of antioxidants to cryopreservation extenders influenced post-thaw sperm characteristics and fertility in Acipenser dabryanus, Acipenser sinensis and Acipenser baerii. Prior to freezing, sperm samples were diluted with a base extender as control or in extender supplemented with catalase (CAT), glutathione (GSH), cysteine (NAC), ascorbic acid (VC) or their paired combinations. Protective concentrations of CAT, GSH and VC in the three species were 25 U/ml, 0.25-0.5 mg/ml and 0.5 mg/ml, respectively. Cysteine showed no protective effect against ROS. The addition of CAT, GSH and VC positively affected either acrosome or membrane integrity of post-thawed sperm in the three species, as well as spermatozoan motility in A. sinensis. The combination of antioxidants did not show a positive synergistic effect. This study suggested that the use of antioxidants in the cryopreservation of sturgeon sperm has potential to decrease intracellular ROS, and consequently preserve acrosome and membrane integrity, as well as spermatozoan motility.

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Section: Introductionmentioning

confidence: 99%

Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species

et al. 2017

Reprod Domestic Animals

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“…The results of many animal researches showed that propolis may relieve the negative effects of oxidative stress on the body's defense system (El-Khawaga et al, 2003;Mannaa et al, 2011;Tatli Seven et al, 2012). Propolis protects the reproductive system from toxicity, especially flavonoids and phenolic compounds are responsible for antioxidant activity and show protective effects against aluminium chloride which caused testicular dysfunction, deterioration in semen quality and testosterone levels (Yousef and Salama, 2009;Ogretmen et al, 2014). Previous studies have reported that propolis provides increments in testosterone level, body and reproductive organ weights, the percentage of motile sperm and normal-shaped sperm and seminal plasma enzymes, and it causes reductions in the levels of free radicals, lactate dehydrogenase in rabbits (Yousef et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

The effects of propolis on sperm quality, reproductive organs and testicular antioxidant status of male rats treated with cyclosporine-A

Baykalır¹,

Seven²,

Gür³

et al. 2016

Anim Reprod

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This study was designed to determine the effects of propolis on the quality of sperm and reproductive organs in male rats treated with cyclosporine-A. In this study, 24 male Sprague-Dawley rats (280-300 g BW) at 8-10 weeks of age were used. The rats were randomly divided into control and 3 treatment groups. Each rat was placed into a cage. During 21 days (experimental period), Group 1 served as control group; Group 2 (CsA) was given 15 mg/kg BW/day of CsA by subcutaneously; Group 3 (P) was given 100 mg/kg BW/day of propolis by gavage; Group 4 (CsA+P) was given 15 mg/kg BW/day of CsA subcutaneously and 100 mg/kg BW/day of propolis by gavage. Administration of CsA to rats decreased sperm motility (P < 0.01) and sperm concentration (P < 0.01), and it increased total abnormal sperm rates (P < 0.05) as compared with the control group. Significant improvements were observed in the sperm motility (P < 0.01) and total abnormal sperm rates (P < 0.05) in CsA+P group. No significant differences were observed in the weights of right and left testes among all groups. The weight values of right and left epididymis were found similar to each other in CsA administrated groups. Seminal vesicle (P < 0.01) and prostat glands weights (P < 0.01) were significantly higher in CsA+P group than CsA group. As a result, it was determined that oral supplementation of 100 mg/kg of propolis had amendatory effect on the quality of sperm and reproductive organs treated with CsA administered rats. CsA treatment caused a significant increase in MDA level (P < 0.01) and significant decreases (P < 0.01) in GSH level and CAT activity when compared with the control group. Ingestion of propolis by CsA-treated rats significantly decreased the MDA level and significantly increased the GSH level when compared with the CsA alone group (P < 0.01).

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“…Extensive searches about these compound classes and their effects on spermatogenesis showed that there were numerous studies that evaluated the antispermatogenic effect of these metabolites; more specifically, the antispermatogenic effect of oleanane saponins was investigated [10,72], the effect of lupeol and lupeol-rich plant extracts on spermatogenesis was examined [73][74][75][76], and the action of diterpenes on testes was discussed [77,78]. There is no available data to confirm the adverse effects of polyphenolics on the male reproductive system except gossypol [3,[79][80][81], and all studies reported about this action emphasized the cytoprotective and antioxidant activities for male sexual organs and sperm after the administration of polyphenolic-rich plants [82][83][84][85]. Therefore, our study focused specifically on oleanane saponins, lupane triterpenes, and phenolic diterpenes, and to elucidate the mechanism of how they affect spermatogenesis, while polyphenolics were outside the scope of this study.…”

Section: Discussionmentioning

confidence: 99%

Testicular Caspase-3 and β-Catenin Regulators Predicted via Comparative Metabolomics and Docking Studies

et al. 2020

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Many routes have been explored to search for effective, safe, and affordable alternatives to hazardous female contraceptives. Herbal extracts and their secondary metabolites are some of the interesting research areas to address this growing issue. This study aims to investigate the effects of ten different plant extracts on testicular spermatogenesis. The correlation between the chemical profile of these extracts and their in vivo effect on male reproductive system was evaluated using various techniques. Approximately 10% of LD50 of hydro-methanolic extracts were orally administrated to rats for 60 days. Semen parameters, sexual organ weights, and serum levels of male sex hormones in addition to testes histopathology, were evaluated. Moreover, metabolomic analysis using (LC-HRESIMS), multivariate analysis (PCA), immunohistochemistry (caspase-3 and β-catenin), and a docking study were performed. Results indicated that three plant extracts significantly decreased epididymal sperm density and motility. Moreover, their effects on testicular cells were also assured by histopathological evaluations. Metabolomic profiling of the bioactive plant extracts showed the presence of diverse phytochemicals, mostly oleanane saponins, phenolic diterpenes, and lupane triterpenes. A docking study on caspase-3 enzyme showed that oleanane saponins possessed the highest binding affinity. An immunohistochemistry assay on β-catenin and caspase-3 indicated that Albizzia lebbeck was the most active extract for decreasing immunoexpression of β-catenin, while Rosmarinus officinalis showed the highest activity for increasing immunoexpression of caspase-3. The spermatogenesis decreasing the activity of A. lebbeck, Anagallis arvensis, and R. officinalis can be mediated via up-regulation of caspase-3 and down-regulation of β-catenin existing in testis cells.

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Protective effects of propolis on cryopreservation of common carp (Cyprinus carpio) sperm

Cited by 29 publications

References 46 publications

Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species

Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species

The effects of propolis on sperm quality, reproductive organs and testicular antioxidant status of male rats treated with cyclosporine-A

Testicular Caspase-3 and β-Catenin Regulators Predicted via Comparative Metabolomics and Docking Studies

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