Protective Effects of Pleurotus tuber-regium on Carbon- Tetrachloride Induced Testicular Injury in Sprague Dawley Rats

Abstract

The high rate of male infertility and the meager resources to manage same in sub Saharan Africa have necessitated the search for cost effective and available alternatives. Mushrooms have been used traditionally in folk medicine and as nutraceuticals. This study has investigated the effect of the wild mushroom Pleurotus tuber-regium on carbon tetrachloride (CCl4) deleterious effects on the reproductive system of male rats. Thirty six rats were divided into six groups of six animals each. Group I (negative control) received 10 ml/kg olive oil intraperitoneal weekly in addition to feed and water ad libitum. Group II (positive control) received CCl4 10 ml/kg (30% in Olive oil) weekly. Group III, IV, and V received 100 mg, 20 0mg, and 500 mg P. tuber-regium (33.3% in feed) daily in addition to 10 ml/kg CCl4 weekly. Group VI received 500 mg P. tuber-regium (33.3% in feed) daily. After 4 weeks, sperm motility, epididymal count and vitality were determined. Serum malondialdehyde (MDA), testosterone, Luteinizing hormone (LH), Follicle stimulating hormone (FSH), prolactin and oestradiol were estimated using enzyme-linked immunosorbent assay (ELISA) kits. Histopathologic examinations of the testis were carried out. Carbon tetrachloride significantly reduced the sperm motility (54.33 ± 3.79%), epididymal count (28.73 ± 2.86 × 106/ml, vitality (4.96 ± 0.62), LH (0.88 ± 0.14), FSH (2.04 ± 0.33), and Testosterone (2.02 ± 0.24) when compared with control (89.33 ± 9.01), 91.91 ± 1.92 × 106/ml, 13.12 ± 0.19, 2.74 ± 0.32, 3.64 ± 0.62, and 4.16 ± 0.23, respectively, which were reversed by P. tuber-regium administration. Co-administration of P. tuber-regium plus CCl4 significantly reduced MDA level. P. tuber-regium showed dose dependent ameliorative activity against CCl4 deleterious action on the testis and may be beneficial in the management of male infertility.

Rutin ameliorates carbon tetrachloride (CCl4)-induced hepatorenal toxicity and hypogonadism in male rats

“…The oxidative stress of the testicular germinal layer is a central cause of the intrinsic apoptotic cascade, which can trigger mitochondrial ROS generation and provoke hypogonadism (Aitken et al, 2014). In addition, CCl 4 reduced the secretion of testosterone, FSH and LH in rats through direct effects on the central nervous system and Leydig cells and/or indirectly by affecting the hypothalamic-pituitary estrogen receptor axis (Okolo, Siminialayi & Orisakwe, 2016; Noureen et al, 2017). CCl 4 enhanced cellular degeneration by increasing the production of H 2 O 2 and nitrite, which resulted in infertility/testicular dysfunction (Sahreen, Khan & Khan, 2013; Ojo et al, 2016).…”

Lead Induced Hepato-renal Damage in Male Albino Rats and Effects of Activated Charcoal

“…The clear serum supernatant was then carefully aspirated and stored in a clean sample bottle for the determination of some biochemical parameters. Rats were sacrificed under ether anesthesia ( Okolo et al, 2016 ) and concussion stunning involving manually applied trauma on the head ( AVMA, 2013 ); the kidney and liver were excised, weighed, rinsed in saline, and preserved in 10% formalin for histopathological study.…”

Cultivation and Nutritional Value of Prominent Pleurotus spp.: An Overview

“…Xu et al demonstrated the cancer effect of polysaccharide-protein complexes derived from PP [105]. On the other hand, other studies demonstrated that b-glucans derived from PTR could induce apoptosis against cancer cells and enhance the immunomodulatory and anti-tumor activities [106]. The high-level intake of Pleurotus mushroom intake can decrease the risk of cancer and other diseases.…”