Protective effects of oleuropein against renal injury oxidative
damage in alloxan-induced diabetic rats; a histological and
biochemical study

Ahmadvand, Hassan; Shahsavari, Gholamreza; Tavafi, Majid; Bagheri, Shahrokh; Moradkhani, Mohamad Reza; Kkorramabadi, Reza Mohammadrezaei; Khosravi, Peyman; Jafari, Maryam; Zahabi, Khadije; Eftekhar, Reza; Soleimaninejad, Maryam; Moghadam, Sanaz

doi:10.15171/jnp.2017.34

Cited by 18 publications

(15 citation statements)

References 23 publications

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“…It has bad effects on kidney tissue during IR (2). Similar studies reported that natural antioxidants play a role in the reduction of NO level in vivo (24). Our results and other studies showed that natural antioxidants could reduce the level of NO and MPO activity.…”

Section: Discussionsupporting

confidence: 92%

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Glutathione ameliorates liver markers, oxidative stress and inflammatory indices in rats with renal ischemia reperfusion injury

et al. 2018

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Introduction: Glutathione (GSH) protects the tissue and cell from oxidative injury. Objectives: In the current study, we investigated the possible effects of GSH on liver markers, oxidative stress and inflammatory indices in rat with renal ischemia reperfusion (RIR) injury. Materials and Methods: Twenty-four adult male Wistar rats were divided into 3 groups (n=8). Group I (the control group), group II (the RIR group) received saline (0.25 mL/d, intraperitoneally; i.p.), group III as the RIR group that received GSH (100 mg/kg/d, i.p.). The treatment with saline or GSH began daily 14 days before RIR induction. RIR was induced by clamping renal pedicles for 45 minutes and 24 hours of reperfusion. Results: RIR significantly increased the serum level of nitric oxide (NO), the serum activities of aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT), the serum and renal levels of malondialdehyde (MDA), and the serum activity of myeloperoxidase (MPO). However, RIR significantly decreased the serum and renal levels of GSH, serum paraoxonase 1 (PON1) activity, and the serum and renal activities of catalase (CAT) and glutathione peroxidase (GPX). GSH administration could significantly improve the serum activities of AST, GGT, MPO, GPX and PON1 and serum levels of NO, renal MDA, GSH levels, and serum and also renal CAT activities. Conclusion: Our study indicated that GSH administration ameliorated RIR injury in rats by improving the activities of liver markers and antioxidant enzymes, the levels of MDA, NO, GSH and MPO activity.

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Section: Discussionsupporting

confidence: 92%

“…The assay of serum nitric oxide (NO) concentration was done by the determination of nitrite which is the end product of NO. The measurement of nitrite was determined in accordance with our study (13).…”

Section: Determination Of No Levelsmentioning

confidence: 99%

Glutathione ameliorates liver markers, oxidative stress and inflammatory indices in rats with renal ischemia reperfusion injury

et al. 2018

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“…31 Our results agree with the results of Ahmadvand et al who reported that oleuropein reduced MPO activity and leukocyte infiltration in the kidney tissues of diabetic rats. 32 F I G U R E 1 0 Histological changes in the kidney tissue in the examined groups. It was demonstrated that OLE might act as a chainbreaking antioxidant, leading to termination of the chain reaction of lipid peroxidation.…”

Section: Discussionmentioning

confidence: 99%

Oleuropein protects against lipopolysaccharide‐induced sepsis and alleviates inflammatory responses in mice

et al. 2020

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Sepsis results from a major systemic inflammatory response and can induce disorders in multiple organs. The present study evaluated the potential protective effects of oleuropein (OLE) against hyperinflammatory responses during lipopolysaccharide (LPS)‐induced sepsis in mice. Sixty male Balb/c mice were randomly categorized into five groups of 12 animals each: control, intraperitoneally injected with OLE (50 mg/kg), injected with LPS (10 mg/kg, intraperitoneal), and two groups administered OLE (25 and 50 mg/kg) for 3 days prior to LPS injection. Twenty‐four hours after lipopolysaccharide injection, the animals were sacrificed. Serum, liver, and kidney tissue samples were collected for biochemical analyses, histopathological examinations, and investigation of inflammation‐related gene expression. OLE pretreatment significantly reduced liver damage parameters (alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase) and kidney damage parameters (blood urea nitrogen, creatinine, and kidney injury molecule‐1) in the septic mice. OLE pretreatment ameliorated LPS‐induced liver and kidney histological changes. OLE significantly mitigated the increased levels of malondialdehyde in the liver and kidneys and reduced levels of reduced glutathione induced by LPS. LPS injection also resulted in increased expression of the proinflammatory cytokines (TNF‐α, IL‐1β, and IL‐6) and inflammation‐related genes (Nos2, Hmgb1, Mpo, Cd46, Map2k4, and Map2k7) in the hepatic and renal tissues. OLE reduced these expressions to ameliorate the inflammatory response. Moreover, OLE pretreatment enhanced the survival rate of septic mice. In conclusion, OLE alleviated the inflammatory response to protect against LPS‐induced sepsis in mice.

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“…This ameliorative property was attributed to the antioxidative and antiatherogenic activities of oleuropein. In a recent study, the ameliorative effects of aqueous olive leaf extracts on kidney functions were also demonstrated in diabetic pregnant mice and their foetuses . Despite of these ethnopharmacological studies, the protective efficiency of this compound in indomethacin‐induced renal injury remains to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

The efficacy of oleuropein against non‐steroidal anti‐inflammatory drug induced toxicity in rat kidney

Koç

Çeriğ

Özek

et al. 2018

Environmental Toxicology

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Indomethacin is generally used in clinical therapeutics as a non-steroidal anti-inflammatory drug.However, its use has been limited due to the gastrointestinal and renal toxic effects of this drug.These toxic effects were associated with not only the inhibition of prostaglandin synthesis but also drug-elevated oxidative stress. To ameliorate these toxicities, natural antioxidants can be used as an alternative and/or combination therapies. Therefore, the current study was conducted to assess the renoprotective effects of oleuropein against indomethacin-induced renal damages. Male Sprague-Dawley rats were pretreated with oleuropein (75, 150, and 300 mg/kg), and then treated with indomethacin (25 mg/kg). To evaluate kidney function, serum blood urea nitrogen, uric acid, and creatinine were measured. In addition, prostaglandin E 2 , tumor necrosis factor-alpha, endothelial nitric oxide synthase, caspase-3, oxidant/antioxidant status, and 8-Oxo-2 0 -deoxyguanosine levels were determined for the antioxidative and anti-inflammatory effects of oleuropein. Tissue sections were also histopathologically assessed. The biochemical and histopathological analysis proved the toxic effects of indomethacin on kidney. However, the pretreatment with oleuropein (300 mg/kg) protects kidney from indomethacin-induced damages. Our study proved that prior administration of oleuropein has renoprotective activity against indomethacin-associated toxicities. K E Y W O R D S8-OHdG, eNOS, histopathologic assessment, oleuropein, prostaglandin E2

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Protective effects of oleuropein against renal injury oxidative damage in alloxan-induced diabetic rats; a histological and biochemical study

Cited by 18 publications

References 23 publications

Glutathione ameliorates liver markers, oxidative stress and inflammatory indices in rats with renal ischemia reperfusion injury

Glutathione ameliorates liver markers, oxidative stress and inflammatory indices in rats with renal ischemia reperfusion injury

Oleuropein protects against lipopolysaccharide‐induced sepsis and alleviates inflammatory responses in mice

The efficacy of oleuropein against non‐steroidal anti‐inflammatory drug induced toxicity in rat kidney

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