Protective effects of N-acetylcysteine and Ginkgo biloba extract on ischaemia-reperfusion-induced hepatic DNA damage in rats

Keleş, Mevlüt Sait; Demirci, Nizamettin; Yıldırım, Abdülkadir; Atamanalp, S. S.; Altınkaynak, Konca

doi:10.1007/s10238-008-0005-1

Cited by 19 publications

(14 citation statements)

References 37 publications

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“…The improvement of the flow in the sinusoids for FL preserved by MP20 can also be ascribed to the action of N-Acetylcysteine (NAC) included in the perfusion medium. NAC is not only a precursor of glutathione, therefore contributing to the protection against oxidative damage, 61,62 but it also has vasorelaxant effects. 63,64 Adding NAC to the liver before CS caused an amelioration of sinusoidal microcirculatory injury after cold I/R in steatotic rat liver.…”

Section: Discussionmentioning

confidence: 99%

Decreased apoptosis in fatty livers submitted to subnormothermic machine-perfusion respect to cold storage

et al. 2011

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Machine perfusion at subnormothermic temperature (20°C), MP20, was developed by Vairetti et al. and showed to afford a better preservation of fatty livers respect to traditional cold storage (CS) in terms of enzyme release into the perfusate, bile production, glycogen stores, energy charge and oxidative stress. Here we investigated whether it also caused decreased cell death by apoptosis. Fatty and lean Zucker rats were submitted to MP20 or CS for 6 h and reperfused normothermically for 2 h. Apoptotic cells were revealed by immunohistochemistry of activated caspase-3 and M30 (new epitope on CK18 degraded by caspase-3) and by the TUNEL assay. Portal pressure was also determined. A statistically significant reduction of hepatocyte apoptosis, but especially of sinusoidal cells was determined for fatty livers submitted to MP20 respect to CS. Portal pressure was significantly lower after MP20 respect to CS. The reduction of sinusoidal cell death by apoptosis without need for anti-apoptotic therapies appears particularly positive since apoptotic sinusoidal cells hinder microcirculation in the sinusoids and are thrombogenic. These results further confirm the potential of MP20 for preserving fatty livers that would be otherwise discarded as grafts, and thus for increasing the donor pool for liver transplantation.

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Section: Discussionmentioning

confidence: 99%

Decreased apoptosis in fatty livers submitted to subnormothermic machine-perfusion respect to cold storage

et al. 2011

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“…No standard dose has been agreed. UD is given internally in most of the studies 9,22. We used a total dose of 2 ml/kg administered intraperitoneally 6,9,10.…”

Section: Discussionmentioning

confidence: 99%

Effects of Urtica dioica on hepatic ischemia-reperfusion injury in rats

Kandiş¹,

Karapolat²,

Yıldırım³

et al. 2010

Clinics

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OBJECTIVES:To evaluate the effects of Urtica dioica on hepatic ischemia‐reperfusion injury.METHODS:Thirty adult male Wistar albino rats were divided into three groups: sham group (group 1), control group (group 2), and Urtica dioica group (group 3). All the rats were exposed to hepatic ischemia for 60 min, followed by 60 min of reperfusion. In group 2, a total of 2 ml/kg 0.9% saline solution was given intraperitoneally. In group 3, a total of 2 ml/kg Urtica dioica was given intraperitoneally. At the end of the procedure, liver tissue and blood samples were taken from all rats. Serum aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, ceruloplasmin, catalase, paraoxonase, arylesterase, and lipid hydroperoxide levels were measured. Liver tissue histopathologies were also evaluated by light microscopy.RESULTS:Serum aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase levels were significantly higher in group 2 than in group 1, and significantly lower in group 3 than in group 2. Also, group 2 had higher serum lipid hydroperoxides and ceruloplasmin levels but lower catalase, paraoxonase, and arylesterase levels than group 1.In group 3, serum lipid hydroperoxides and ceruloplasmin levels were significantly lower, and catalase, paraoxonase, and arylesterase levels were higher than those in group 2. Histopathological examination showed that liver tissue damage was significantly decreased in group 3 compared with group 2.CONCLUSIONS:Urtica dioica has a protective effect on the liver in hepatic ischemia‐reperfusion‐injured rats.

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“…Moreover, it could be seen that these antidotes exhibited the role of resisting fluorosis mainly through the mechanism of inhibiting oxidative stress and DNA damage. Some animal researches showed that NAC could prevent postischaemic reperfusion injury in hepatic tissue, CCl4 -induced liver damage and brain intoxication induced by propionic acid (PPA) by reducing DNA damage and oxidative stress [46][47][48]. Moreover recent human studies implied that NAC could attenuate pesticide-induced DNA damage in human peripheral blood mononuclear cells, and age-related increases in genetic damage in human T lymphocytes, in vitro and ex vivo [49,50].…”

Section: Nac Pretreatment Reduced Fluoride-induced Oxidative Stress Amentioning

confidence: 99%

Ameliorative effects of N-acetylcysteine on fluoride-induced oxidative stress and DNA damage in male rats’ testis

Feng

Huang

Yang

et al. 2015

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Protective effects of N-acetylcysteine and Ginkgo biloba extract on ischaemia-reperfusion-induced hepatic DNA damage in rats

Cited by 19 publications

References 37 publications

Decreased apoptosis in fatty livers submitted to subnormothermic machine-perfusion respect to cold storage

Decreased apoptosis in fatty livers submitted to subnormothermic machine-perfusion respect to cold storage

Effects of Urtica dioica on hepatic ischemia-reperfusion injury in rats

Ameliorative effects of N-acetylcysteine on fluoride-induced oxidative stress and DNA damage in male rats’ testis

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