Protective Effects of Mangosteen Extract on H2O2-Induced Cytotoxicity in SK-N-SH Cells and Scopolamine-Induced Memory Impairment in Mice

Abstract

Mangosteen extracts (ME) contain high levels of polyphenolic compounds and antioxidant activity. Protective effects of ME against β-amyloid peptide (Aβ), induced cytotoxicity have been reported. Here, we further studied the protective effects of ME against oxidative stress induced by hydrogen peroxide (H2O2) and polychlorinated biphenyls (PCBs), and demonstrated the protection against memory impairment in mice. The cytoprotective effects of ME were measured as cell viability and the reduction in ROS activity. In SK-N-SH cell cultures, 200 μg/ml ME could partially antagonize the effects of 150 or 300 µM H2O2 on cell viability, ROS level and caspase-3 activity. At 200, 400 or 800 µg/ml, ME reduced AChE activity of SK-N-SH cells to about 60% of the control. In vivo study, Morris water maze and passive avoidance tests were used to assess the memory of the animals. ME, especially at 100 mg/kg body weight, could improve the animal’s memory and also antagonize the effect of scopolamine on memory. The increase in ROS level and caspase-3 activity in the brain of scopolamine-treated mice were antagonized by the ME treatment. The study demonstrated cytoprotective effects of ME against H2O2 and PCB-52 toxicity and having AChE inhibitory effect in cell culture. ME treatment in mice could attenuate scopolamine-induced memory deficit and oxidative stress in brain.

Protective effect ofLepidium sativumseed extract against hydrogen peroxide-induced cytotoxicity and oxidative stress in human liver cells (HepG2)

“…In this study, the restoration of the intra-cellular ROS level by pre-treatment with LSE showed the protective effects of various concentrations of LSE against H 2 O 2 -induced ROS generation in HepG2. Our results are in agreement with the previous reports showing that the administration of natural products suppress the increased level of intra-cellular ROS generation (Farshori et al, 2013;Molina et al, 2003;Sattayasai et al, 2013;You et al, 2010). It is known that apoptosis-signaling cascades can be initiated by mitochondria impairment (Taveira et al, 2014).…”

“…crude extracts from natural products exhibited significant cyto-protection against the H 2 O 2 in PC12 and SK-N-SH cells Sattayasai et al, 2013) and in liver cells against other toxicants (Alia et al, 2006;Goya et al, 2007;Martin et al, 2008;Verma et al, 2013).…”

“…H 2 O 2 is known to induce oxidative stress in a variety of cells Siddiqui et al, 2011;Sattayasai et al, 2013). There is a plethora of evidence documenting the essential involvement of oxidative stress-induced damages and increased levels of H 2 O 2 in a variety of diseases (Spencer et al, 2009;Yamashita et al, 2008).…”

“…A number of in vitro studies have demonstrated that oxidative stress induced by chemical oxidants, such as hydrogen peroxide (H 2 O 2 ), leads to cell death (Cai et al, 2008;Hwang & Yen, 2008;Kim et al, 2009;Siddiqui et al, 2011). H 2 O 2 has also been reported to induce apoptotic changes, which subsequently lead to death in a variety of cell systems (Jung et al, 2006;Kanno et al, 2003;Sattayasai et al, 2013), including human liver cells (HepG2) (Alia et al, 2005;Chen et al, 2011). Therefore, we have selected H 2 O 2 to induce the oxidative stress-mediated cytotoxicity in the HepG2.…”

Anti-Glycemic and Anti-Hepatotoxic Effects of Mangosteen Vinegar Rind from Garcinia mangostana Against HFD/STZ-Induced Type II Diabetes in Mice

“…In vitro and in vivo studies have proven that Garcinia mangostana is characterized by antioxidative and cytoprotective activities due to the presence of phenolic compounds [Phyu & Tangpong, 2014;Sattayasai et al, 2013]. Several studies found that the polyphenolic compounds found in Aegle marmelos, Commiphora mukul, green tea, cinnamon and ginger have hepatoprotective properties in different animal models [Elgawish et al, 2015;Ismail, 2014;Ramesh et al, 2015;Suriyamoorthy et al, 2014].…”