Protective effects of Lycium barbarum polysaccharides against carbon tetrachloride-induced hepatotoxicity in precision-cut liver slices in vitro and in vivo in common carp (Cyprinus carpio L.)

Liu, Ying-Juan; Cao, Liping; Du, Junbao; Jia, Rui; Wang, Jiahao; Xu, Pao; Yin, Guojun

doi:10.1016/j.cbpc.2014.12.005

Cited by 35 publications

(17 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…H 2 O 2 , a typical oxidant, has been used to study the response of cells to oxidative stress [ 28 ]. MDA is the product of lipid peroxidation and a classic indicator of oxidative stress and SOD is one of the major enzymes responsible for the inactivation of superoxide and hydrogen peroxid [ 29 ]. The results of this study showed that Q7R could increase the activity of SOD and inhibit the generation of MDA in L-02 cells.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Activity and Hepatoprotective Potential of Quercetin 7-Rhamnoside In Vitro and In Vivo

et al. 2018

View full text Add to dashboard Cite

Hypericum japonicum is traditionally used as a folk medicine to treat cholestasis and hepatitis. Quercetin 7-rhamnoside (Q7R) is one of the main flavonoid components of Hypericum japonicum and has been rarely studied. The aim of the present study was to evaluate the antioxidant activity and hepatoprotective potential of Q7R. In the in vitro experiments, DPPH, ABTS and ferric reducing antioxidant power (FRAP) assays were first performed to assess the antioxidant properties of Q7R, and then a H2O2-induced oxidative damage cellular model was used to determine the cytoprotective and antioxidant properties of Q7R in human liver L-02 cells. In the in vivo experiment, the hepatoprotective activity of Q7R was evaluated by carbon tetrachloride (CCl4)-induced liver damage model in mice. The results of the three in vitro assays (DPPH, ABTS and FRAP) demonstrated that Q7R significantly exhibited antioxidant activity. The cell experiment results showed that Q7R possessed cytoprotective and antioxidant effects on H2O2-treated L-02 cells. In the in vivo experiments, Q7R suppressed the up-regulation of serum activities of ALT, AST, LDH and triglyceride (TG) levels with dose-dependency. Q7R down-regulated the production of MDA and increased the hepatic GSH content and antioxidant enzymes CAT activities. Hepatic morphological analysis was also performed to confirm the biochemical changes. In summary, these results suggested that Q7R could be considered as a potential source of natural antioxidants, and may become a promising candidate for the treatment of liver injury in the future.

show abstract

Section: Discussionmentioning

confidence: 99%

Antioxidant Activity and Hepatoprotective Potential of Quercetin 7-Rhamnoside In Vitro and In Vivo

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Biochemical parameters such as glucose and protein levels and inorganic ions (Na + , K + , and Cl − ) are the indicators of energy levels of the fish. Enzymological parameters such as LDH, GOT and GPT serves as feasible biomarkers to study the integrity of the immune system and tissue damage in fish (Liu et al., 2015; Poopal et al., 2017).…”

Section: Introductionmentioning

confidence: 99%

Chronic amoxicillin exposure affects Labeo rohita: assessment of hematological, ionic compounds, biochemical, and enzymological activities

Umamaheswari

Renuka

Ramesh

et al. 2019

Heliyon

View full text Add to dashboard Cite

Labeo rohita were exposed to amoxicillin at a concentration of 1 mg/L (Treatment -I) and 0.5 mg/L (Treatment-II) for a period of 35 days. Numerous alterations were found in amoxicillin treatment groups when compared to the control group. Hemoglobin (Hb), hematocrit (Hct), and erythrocytes (RBCs) levels were significantly ( P < 0.05) decreased. Leukocytes (WBC), mean cell volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) levels were significantly ( P < 0.05) increased. In the plasma and gill tissues, ionic compounds (sodium, potassium, and chloride) levels were significantly declined throughout the treatment period. The plasma biochemical profiles were significantly altered: glucose level remained low (except at the end of 7 th day in Treatment -I) till 35 days of the treatment period. Biphasic trend occurred in the protein level, significant increase was observed on 7 th and 28 th day (Treatment -I and -II), and 35 th day (Treatment -I), and in remaining days its level was found to be decreased. Glutamate oxaloacetate transaminase (GOT) activity in the plasma was inhibited significantly, whereas in the gill, liver, and kidney tissues the enzyme activity was elevated. Plasma glutamate pyruvate transaminase (GPT) activity was inhibited throughout the study period. GPT activity in the gill was found to be elevated during the treatment period. Liver GPT activity was elevated in all the treatments except 28 th (Treatment-I) and 35 th day (Treatment-I, and II). GPT activity in the kidney was elevated (except 14 th day in Treatment-II). Lactate dehydrogenase (LDH) activity was inhibited in plasma (except 14 th day in Treatment-II), gill, liver (except 7 th day in Treatment-I), and kidney tissues significantly ( P < 0.05). The present study emphasizes that amoxicillin at 1 and 0.5 mg/L concentrations affects the hematological/biochemical/electrolytes/enzymological parameters of fish and these biomarkers serve as an effective test system for environmental risk assessment of pharmaceuticals in the aquatic environment.

show abstract

“…Studies have shown that increased ROS production in allergic respiratory diseases contributes, in part, to tissue damage at sites of inflammation [34]. MDA is the product of lipid peroxidation and a classic indicator of oxidative stress and SOD is one of the major enzymes responsible for the inactivation of superoxide and hydrogen peroxide [35]. GSH, reduced GSH, is a key antioxidant responsible for primary defense system for protection from oxidative damage [36].…”

Section: Discussionmentioning

confidence: 99%

Viwithan: A Standardized Ashwagandha Extract Ameliorates Ovalbumininduced Airway-Inflammation and Oxidative Stress in Mouse Model

Hs¹,

Hv²,

Illuri³

et al. 2021

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objective: Withania somnifera, commonly known as Ashwagandha, Indian ginseng, has been used in Ayurvedic and indigenous medicinal preparations for various disease conditions since long time. In the present study, we investigated the protective effects of Viwithan, a standardized proprietary extract from Ashwagandha roots, against airway-inflammation and oxidative stress modulation in an ovalbumin (OVA)-induced murine model of inflammation. Methods: Allergic asthma was initiated in BALB/c mice by sensitizing with OVA on days 1 and 14, followed by intranasal challenge with OVA on days 27, 28, and 29. Mice were administered Viwithan (200 and 400 mg/kg) by oral gavage before challenge. Then, mice were evaluated for the presence of airway inflammation, production of allergen-specific cytokine response, lung pathology, and oxidative stress modulation. Results: The results showed that treatment with Viwithan attenuated OVA-induced lung inflammation in mice. Viwithan significantly attenuated inflammatory cell infiltration into the bronchoalveolar lavage fluid and markedly reduced the levels of pro-inflammatory cytokines, interleukin-10, and transforming growth factor-β1 in lung tissues. Viwithan treatment considerably reduced the lung weight in OVA-sensitized mice. Viwithan markedly attenuated the OVA-induced generation of reactive oxygen species in lung tissues. Conclusion: Together, these results suggested that Viwithan alleviates OVA-induced airway-inflammation and oxidative stress, highlighting the potential of standardized Ashwagandha extract as a useful therapeutic agent for pulmonary fibrosis management.

show abstract

Protective effects of Lycium barbarum polysaccharides against carbon tetrachloride-induced hepatotoxicity in precision-cut liver slices in vitro and in vivo in common carp (Cyprinus carpio L.)

Cited by 35 publications

References 41 publications

Antioxidant Activity and Hepatoprotective Potential of Quercetin 7-Rhamnoside In Vitro and In Vivo

Antioxidant Activity and Hepatoprotective Potential of Quercetin 7-Rhamnoside In Vitro and In Vivo

Chronic amoxicillin exposure affects Labeo rohita: assessment of hematological, ionic compounds, biochemical, and enzymological activities

Viwithan: A Standardized Ashwagandha Extract Ameliorates Ovalbumininduced Airway-Inflammation and Oxidative Stress in Mouse Model

Contact Info

Product

Resources

About