Antioxidant Activity and Hepatoprotective Potential of Quercetin 7-Rhamnoside In Vitro and In Vivo

Abstract

Hypericum japonicum is traditionally used as a folk medicine to treat cholestasis and hepatitis. Quercetin 7-rhamnoside (Q7R) is one of the main flavonoid components of Hypericum japonicum and has been rarely studied. The aim of the present study was to evaluate the antioxidant activity and hepatoprotective potential of Q7R. In the in vitro experiments, DPPH, ABTS and ferric reducing antioxidant power (FRAP) assays were first performed to assess the antioxidant properties of Q7R, and then a H2O2-induced oxidative damage cellular model was used to determine the cytoprotective and antioxidant properties of Q7R in human liver L-02 cells. In the in vivo experiment, the hepatoprotective activity of Q7R was evaluated by carbon tetrachloride (CCl4)-induced liver damage model in mice. The results of the three in vitro assays (DPPH, ABTS and FRAP) demonstrated that Q7R significantly exhibited antioxidant activity. The cell experiment results showed that Q7R possessed cytoprotective and antioxidant effects on H2O2-treated L-02 cells. In the in vivo experiments, Q7R suppressed the up-regulation of serum activities of ALT, AST, LDH and triglyceride (TG) levels with dose-dependency. Q7R down-regulated the production of MDA and increased the hepatic GSH content and antioxidant enzymes CAT activities. Hepatic morphological analysis was also performed to confirm the biochemical changes. In summary, these results suggested that Q7R could be considered as a potential source of natural antioxidants, and may become a promising candidate for the treatment of liver injury in the future.

Antioxidant activities of anastatin A & B derivatives and compound 38c's protective effect in a mouse model of CCl4-induced acute liver injury

“…The levels of serum AST, ALT and LDH activities reects damage to hepatocytes and were considered to be highly sensitive and fairly specic preclinical and clinical biomarkers of hepatotoxicity. 46 It is exciting that high dose (200 mg kg À1 ) and medium dose (100 mg kg À1 ) of compound 38c show good protection, which can reverse the increase of AST, ALT, LDH in serum caused by CCl 4 , the current results are also consistent with previous reports. GSH, an important part of the main defense system, can scavenge free radicals in the body.…”

Hepatoprotective and Nephroprotective Activity of Artemisia absinthium L. on Diclofenac-induced Toxicity in Rats

“…In Artemisia annua, hepatoprotective activity is connected to their content of hydroxycinnamoyl quinic acids and flavonoids. 42 An important mechanism of hepatoprotective activity is related to its ability to transfer hydrogen to free radicals, activate antioxidant enzymes, and inhibit oxidases 43 . Methanol is the solvent capable of extracting the greatest amount of phenolic compounds 44 , a type of compound that presents a high antioxidant capacity that is present in A. absinthium 45 , however, bioguided phytochemical prospecting is required to determine the metabolite(s) responsible for this activity and its subsequent determination of its mechanism of action by which it exerts its pharmacological action.…”

Meroterpenoid-Rich Fraction of the Ethanolic Extract from Sargassum serratifolium Suppressed Oxidative Stress Induced by Tert-Butyl Hydroperoxide in HepG2 Cells

“…Recent studies have suggested that excess free radicals play a key role in the development and progression of liver injuries [ 17 ]. Thus, natural antioxidants have attracted attention for their safety, by preventing oxidative liver injuries, and the dietary consumption of antioxidants is recommended to prevent liver diseases [ 18 ]. Thus, we investigated the hepatoprotective effect of MES on t -BHP-treated HepG2 cells.…”