Protective effects of low-magnitude high-frequency vibration on high glucose-induced osteoblast dysfunction and bone loss in diabetic rats

Abstract

Objective Low-magnitude high-frequency vibration (LMHFV) has been reported to be capable of promoting osteoblast proliferation and differentiation. Reduced osteoblast activity and impaired bone formation were related to diabetic bone loss. We investigated the potential protective effects of LMHFV on high-glucose (HG)-induced osteoblasts in this study. In addition, the assessment of LMHFV treatment for bone loss attributed to diabetes was also performed in vivo. Method MC3T3-E1 cells induced by HG only or treated with LMHFV were treated in vitro. The experiments performed in this study included the detection of cell proliferation, migration and differentiation, as well as protein expression. Diabetic bone loss induced by streptozotocin (STZ) in rats was established. Combined with bone morphometric, microstructure, biomechanical properties and matrix composition tests, the potential of LMHFV in treating diabetes bone loss was explored. Results After the application of LMHFV, the inhibiting effects of HG on the proliferation, migration and differentiation of osteoblasts were alleviated. The GSK3β/β-catenin pathway was involved in the protective effect of LMHFV. Impaired microstructure and biomechanical properties attributed to diabetes were ameliorated by LMHFV treatment. The improvement of femur biomechanical properties might be associated with the alteration of the matrix composition by the LMHFV. Conclusion LMHFV exhibited a protective effect on osteoblasts against HG by regulating the proliferation, migration and differentiation of osteoblasts. The function of promoting bone formation and reinforcing bone strength made it possible for LMHFV to alleviate diabetic bone loss.

A Review - Does Low Magnitude High-Frequency Vibration (LMHFV)Worth for Fracture Recovery Compared with Pulsed Electromagnetic Field (PEMF) Based Magnetotherapy Method?

“…The mineralized callus is replaced with mineralized bone during the final stage of bone healing. The bone is then modelled and remodelled to restore its previous form and biomechanical competency [41]. The second phase, referred FIG.…”

“…Low-magnitude high-frequency vibration (LMHFV), a non-pharmacological medical treatment, has shown to positively impact bone induction and remodelling for various muscle diseases in animal studies [41][42][43][44][45][46][47][48][49][50][51]. Show that LMHFV is a valuable method for enhancing osseointegration clinically, particularly for osteoporosis [43].…”

“…In contrast to the PEMF method, the LMHFV method is also used in the fracture therapy process using the magnetic field method [40][41][42][43][44][45]. When LMHFV was performed at 35 Hz, 0.3 g, the healing process for both normal and osteoporotic bone demonstrated an improved acceleration of the healing process by reformed production of callus and mineralization [46][47][48][49][50][51][52][8][9][10][11][12].…”

Do Vibrational Forces Induce an Anabolic Effect in the Alveolar Bone of Animal Models? A Systematic Review

“…Vibrations are cyclic forces performing a physical stimulus that has been shown promote bone formation through osteoblast differentiation and proliferation [28]. Th phenomenon may be possible because the bone is a mechanosensitive tissue, and bo…”

“…Vibrations are cyclic forces performing a physical stimulus that has been shown to promote bone formation through osteoblast differentiation and proliferation [28]. This phenomenon may be possible because the bone is a mechanosensitive tissue, and bone cells react to a biomechanical environment by activating molecular signaling pathways that regulate matrix production, differentiation, and proliferation [16].…”