Protective Effects of Lactobacillus plantarum Lac16 on Clostridium perfringens Infection-Associated Injury in IPEC-J2 Cells

Zhou, Yuguang; Wang, Baikui; Wang, Qi; Tang, Li; Zou, Peng; Zeng, Zihan; Zhang, Huihua; Gong, Li; Li, Weifen

doi:10.3390/ijms222212388

Cited by 11 publications

(5 citation statements)

References 103 publications

(133 reference statements)

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“…In general, antimicrobial activity is the main mode of action of probiotics to prevent bacterial diseases ( Compaore et al., 2013 ; Nami et al., 2015 ; Du et al., 2019 ; Algburi et al., 2021 ; Zhou et al., 2021 ). Because no inhibitory activity on the growth of the S. braenderup H9812 was detected in vitro , prevention of B. subtilis LF11 from S. braenderup H9812 infection might be caused by other possible mechanisms including intestinal barrier ( Rao and Samak, 2013 ), coaggregation ( Collado et al., 2007 ), and signal interference ( Piewngam et al., 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Probiotic Bacillus subtilis LF11 Protects Intestinal Epithelium Against Salmonella Infection

Zhang

et al. 2022

Front. Cell. Infect. Microbiol.

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Enteric diseases caused by Salmonella are prevalent in poultry farming. With the forbiddance of antibiotics in feedstuff industry, Bacillus subtilis (B. subtilis) preparation as antibiotic alternatives against Salmonella infection has gained increasing attention recently. However, the protection modes of B. subtilis against Salmonella infection in broilers are strain-specific. In this study, probiotic B. subtilis LF11 significantly reduced diarrhea and mortality of broilers caused by Salmonella braenderup (S. braenderup) in spite of no inhibition effect on it in vitro. Here, the intestinal epithelial cells NCM460 were incubated to explore the protection of B. subtilis LF11 on intestinal epithelium against Salmonella. The results revealed that B. subtilis LF11 showed obvious exclusion activity with the decrease of adhesion and invasion of S. braenderup to NCM460 cells, accordingly with the increase of NCM460 cell survival compared with S. braenderup challenge alone. Meanwhile, RT-PCR and Western blot proved that the gene transcription and expression levels of four tight junction proteins in NCM 460 cells were upregulated, which was further confirmed by immunofluorescence observation. Besides, B. subtilis LF11 downregulated the gene transcription levels of the proinflammatory cytokines IL-6, IL-8, and TNF-α induced by S. braenderup H9812. ELISA analysis also verified that B. subtilis LF11 reduced the IL-8 production significantly. In general, B. subtilis LF11 has the ability to protect the intestinal epithelium against Salmonella infection by reducing the Salmonella adhesion and invasion, enhancing the intestinal barrier and attenuating the enterocyte inflammatory responses, and has the potential as probiotics to prevent enteric diseases in broilers.

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Section: Discussionmentioning

confidence: 99%

Probiotic Bacillus subtilis LF11 Protects Intestinal Epithelium Against Salmonella Infection

Zhang

et al. 2022

Front. Cell. Infect. Microbiol.

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“…This effect has been described as one of the mechanisms involved in the augmented growth performance in post-weaning piglets upon the treatment with the ZLP001 strain (Wang et al, 2012). Similar studies have demonstrated the ability of lactobacilli to modulate host defence peptides in porcine intestinal epithelial cells including L. reuteri I5007 (Liu et al, 2017), L. salivarius B1 (Zhang et al, 2011), and L. plantarum Lac16 (Zhou et al, 2021).…”

Section: Immunoregulatory Properties Of L Plantarum Isolatesmentioning

confidence: 64%

In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milk

Ohgi,

Saha,

Zhou

et al. 2024

Front. Microbiol.

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The emergence and spread of antibiotic resistance threat forced to explore alternative strategies for improving the resistance to pathogens in livestock production. Probiotic lactic acid bacteria represent an alternative for this objective. In this study, seven Lactiplantibacillus plantarum strains from porcine colostrum and milk were isolated, identified and characterized in terms of their abilities to modulate immunity in porcine intestinal epithelial (PIE) cells. Then, two potential immunoregulatory strains were studied in terms of their ability to utilize and grow in wakame (Undaria pinnafida). Isolates were identified by 16S rRNA gene and evaluated by studying their interaction with PIE cells. The expressions of peptidoglycan recognition proteins (PGRPs), nucleotide-binding oligomerization domain (NODs), host defense peptides (pBD), and type I interferons (IFNs) were evaluated by RT-qPCR. The strain 4M4417 showed a remarkable capacity to differentially regulate the expression of PGRP1, PGRP3, NOD1, NOD2, and pBD1 in PIE cells. On the other hand, the strain 4M4326 was the most efficient to improve the expression of IFN-α and IFN-β in PIE cells challenged with poly (I:C). Both L. plantarum 4M4326 and 4M4417 were characterized in terms of their ability to utilize wakame. Results demonstrated that both strains efficiently grew in wakame-based broth. Our results suggest that L. planatrum 4M4326 and 4M4417 are interesting candidates to develop immunomodulatory feeds based on wakame utilization. These new immunosynbiotic feeds could help to reduce severity of intestinal infections and improve immune health status in pigs.

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“…Huang et al demonstrated that Pediococcus can improve gastrointestinal motility and promote the production of beneficial bacteria that generate short-chain fatty acids [78]. Zhou et al also reported that Lactobacillus can attenuate cytotoxicity and intestinal barrier damage induced by Clostridium perfringens [79]. Furthermore, the abundance of Clostrid-ium_sensu_stricto was higher in fermentations treated with TCM-3 or TDM-2 than in the control group, which has also been reported to enhance short-chain fatty acid production [80].…”

Section: Discussionmentioning

confidence: 97%

High Antioxidant Capacity of Lacticaseibacillus paracasei TDM-2 and Pediococcus pentosaceus TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut Modulation

Yang

Fan

et al. 2023

Foods

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Probiotic supplementation is a key therapeutic strategy for promoting gut health and maintaining gut homeostasis by modulating functional microbiota. In this study, we isolated two lactic acid bacteria (LAB) strains, Pediococcus pentosaceus TCM-3 and Lacticaseibacillus paracasei TDM-2, from Qinghai-Tibetan plateau, and evaluated their probiotic properties and antioxidant bioactivity. In which, TDM-2 had higher T-AOC activity than either TCM-3 or LGG (4.10 μmol/mL vs. 3.68 and 3.53 μmol/mL, respectively, p < 0.05). These strains have shown high antioxidant activity compared to the LAB strains and were found to be acid and bile salt tolerant, confronting the safety issues of antibiotic resistance and the capability of surviving in simulated gastric and intestinal juices. In vitro fermentation experiments with human gut microbiota revealed significant differences in microbial community composition between samples supplemented with TCM-3 and TDM-2 and those without. The addition of these two strains resulted in an enrichment of beneficial taxa, such as the Pediococcus, Lactobacillus, and Clostridium_sensu_strictos at the genus level, and Firmicutes and Proteobacteria at the phylum level. Notably, the TCM-3 group exhibited higher short-chain fatty acid production than the TDM-2 group and untreated controls (acetic acid at 12 h: 4.54 mmol L−1 vs. 4.06 mmol L−1 and 4.00 mmol L−1; acetic acid at 24 h: 4.99 mmol L−1 vs. 4.90 mmol L−1 and 4.82 mmol L−1, p < 0.05). These findings demonstrate that LAB supplementation with high antioxidant capacity and probiotic properties can promote gut health by modulating functional microbiota and is enriching for beneficial taxa. Our study provides guidance for therapeutic strategies that use novel LAB strains to maintain gut homeostasis and functional microbiota modulation.

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Protective Effects of Lactobacillus plantarum Lac16 on Clostridium perfringens Infection-Associated Injury in IPEC-J2 Cells

Cited by 11 publications

References 103 publications

Probiotic Bacillus subtilis LF11 Protects Intestinal Epithelium Against Salmonella Infection

Probiotic Bacillus subtilis LF11 Protects Intestinal Epithelium Against Salmonella Infection

In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milk

High Antioxidant Capacity of Lacticaseibacillus paracasei TDM-2 and Pediococcus pentosaceus TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut Modulation

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