Protective Effects of Lactic Acid Bacteria Against TLR4 Induced Inflammatory Response in Hepatoma HepG2 Cells Through Modulation of Toll-Like Receptor Negative Regulators of Mitogen-Activated Protein Kinase and NF-κB Signaling

Kanmani, Paulraj; Kim, Hojun

doi:10.3389/fimmu.2018.01537

Cited by 43 publications

(42 citation statements)

References 50 publications

(62 reference statements)

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“…We found that 5%LHFM_Cit-4mM was able to induce all negative regulators mRNA expression in non-LPS treated cells, although IRAK-M and Tollip were not statistically significant (P = 0.55 and P = 0.17, respectively). When incubated with LPS, it resulted in activation of A20 and IRAK-M expression in this study that is consistent with previous findings 16 . However, other studies by Li, et al 57 and Finamore, et al 58 demonstrated that A20, Tollip and IRAK-M expressions were suppressed in ETEC infection.…”

Section: Discussionsupporting

confidence: 93%

The protective effects of enriched citrulline fermented milk with Lactobacillus helveticus on the intestinal epithelium integrity against Escherichia coli infection

El-Nezami

Shah

2020

Sci Rep

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This study examined the protective effects of citrulline enriched-fermented milk with live Lactobacillus helveticus ASCC 511 (LH511) on intestinal epithelial barrier function and inflammatory response in IPEC-J2 cells caused by pathogenic Escherichia coli. Five percent (v/v) of fermented milk with live LH511and 4 mM citrulline (5%LHFM_Cit-4mM) significantly stimulated the population of IPEC-J2 cells by 36% as determined by MTT assay. Adhesion level of LH511 was significantly increased by 9.2% when incubated with 5%LHFM_Cit-4mM and 5%LHFM_Cit-4mM reduced the adhesion of enterohemorrhagic (EHEC) and entero-invasive (EIEC) E. coli in IPEC-J2 cells by 35.79% and 42.74%, respectively. Treatment with 5%LHFM_Cit-4mM ameliorated lipopolysaccharide (LPS) from E. coli O55:B5 induced activated inflammatory cytokines expression (TNF-α, IL-6 and IL-8) and concentration (IL-6 and IL-8) and early apoptosis. It restored the transepithelial electrical resistance (TEER) and regulated the expression and distribution of tight junction (TJ) proteins (zonula occluden-1 (ZO-1), occludin and claudin-1), tolllike receptors (TLRs) (TLR2 and TLR4) and negative regulators of TLRs signalling pathway (A20 and IRAK-M). In conclusion, our findings suggested that 5%LHFM_Cit-4mM might have the positive effects on improving and maintaining the intestinal epithelial cell integrity and inflammatory response under both normal and pathogenic LPS-stimulated conditions.Probiotics are defined as live micro-organisms that contribute health-promoting effects 1 . These organisms have been extensively investigated for their health-promoting effects on intestinal tract and modulating the intestinal epithelial barrier functions by several mechanisms, such as prevention against pathogenic adhesion, reinforcement of tight junctions (TJ), protective effects against damage on TJ, suppression of inflammatory cytokines and anti-apoptotic effects 2 .Pathogenic infection occurs when pathogen adheres to host intestinal epithelium 3 . The infection of pathogenic Escherichia coli strains, such as enterohemorrhagic (EHEC) and enteroinvasive (EIEC) E. coli, begins with the colonization and adherence to the intestinal mucosal surface and thus causing damage to the host 4 . The ability of probiotics to adhere to the intestinal epithelial cells is considered as a possible mechanism contributing to the inhibition effect against pathogenic adhesion by competing the adhesion site or/and for nutrients 3 or producing antimicrobial agents 5 . Several Lactobacillus strains, such as L. rhamnosus GG, L. fermentum, L. acidophilus and L. plantarum, have been demonstrated to adhere to the intestinal mucus and markedly diminish the adhesion of Bacteroides vulgatus, Clostridium histolyticum, Enterobacter aerogenes and Staphylococcus aureus 3 . Impaired intestinal barrier functions following pathogenic infection include TJ dysfunction, including increased epithelial permeability and damages to epithelial structure, trigger of pro-inflammatory response and excessive apoptosis. With regard to...

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Section: Discussionsupporting

confidence: 93%

The protective effects of enriched citrulline fermented milk with Lactobacillus helveticus on the intestinal epithelium integrity against Escherichia coli infection

El-Nezami

Shah

2020

Sci Rep

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“…The purity of the RNA was checked, after which it was used to synthesize cDNA using a BioRad thermal cycler (BIORAD, Hercules, CA, USA). Next, RT-PCR was performed using a 7300 real-time PCR system (Roche Applied Science, Indianapolis, IN, USA) with SYBR green and primers [27]. The reaction mixture had a total volume of 20 μl that contained 1 μl of cDNA and 19 μl of master mix including SYBR green and both forward and reverse primers (1 pmol/ μl).…”

Section: Real-time Polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Beneficial effect of immunobiotic strains on attenuation of Salmonella induced inflammatory response in human intestinal epithelial cells

Kanmani

Kim

2020

PLoS ONE

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Probiotic bacteria have the ability to modulate host immune responses and have potent therapeutic functional effects against several diseases, including inflammatory diseases. However, beneficial effects of probiotics are strain specific and their interactions with host immune cells to modulate inflammatory response are largely unknown. Intestinal epithelial cells (IECs), which are the first line of defense against invading pathogens, and connects between commensals/probiotics and immune system; therefore, in this study, we used human IECs to assess the probiotic effects of three selected Lactobacillus strains in vitro. An HT-29 colonic epithelial cell and HT-29/blood mononuclear cells co-culture system were stimulated with Lactobacillus followed by Salmonella for different hours, after which the mRNA level of cytokines, β-defensin-2 and negative regulators for TLR signaling and protein levels of ZO-1 and IκB-α were analyzed by real-time polymerase chain reaction and western blot analysis. L. brevis decreased Salmonella induced IL-6, IL-8, MCP-1 and IL-1β levels, whereas L. pentosus suppressed IL-6 and MCP-1 in HT-29 cells. Moreover, L. brevis was able to increase the mRNA levels of A20, Tollip, SIGIRR and IRAKM, while L. pentosus reduced the levels of A20, and IRAKM in response to Salmonella. In addition, decrease in protein level of TNF-α and increase in mRNA level of IL-10 was observed in L. brevis and L. pentosus treated HT-29 cells. Lactobacillus strains were differentially modulated ZO-1 and p-IκB-α in HT-29 cells treated with Salmonella. Overall, the results of this study indicate that Lactobacillus strains attenuate Salmonella induced inflammatory responses through beneficial modulation of TLR negative regulators and the NF-κB pathway.

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“…L. plantarum revealed some anti-inflammatory activity through inhibition of CXCL8 expression and increase of the anti-inflammatory cytokine IL-10 in Caco-2 cells [ 54 , 55 ] while a combination of selected LAB ( L. plantarum DU1, L. farciminis , Weissella cibaria DU1, and L. pentosus ) reduces hepatic steatosis and attenuates the inflammatory response in vitro through IL-6, CXCL8, CCL2, and TNF-α inhibition via modulation of Toll-like Receptor (TLR) negative regulators of the MAPK and NF-κB pathways [ 56 ]. Ex vivo studies in ileal and colonic mucosa from 10 post-infectious irritable bowel syndrome patients (PI-IBS), and 10 healthy controls pretreated with L. casei DG (LC-DG) showed that LC-DG significantly reduced pro-inflammatory cytokines IL-1α, IL-6 and IL-8 mRNA levels as well as TLR-4 protein expression after lipopolysaccharide (LPS) stimulation [ 57 ].…”

Section: Resultsmentioning

confidence: 99%

Effect of Lactobacillus acidophilus Fermented Broths Enriched with Eruca sativa Seed Extracts on Intestinal Barrier and Inflammation in a Co-Culture System of an Enterohemorrhagic Escherichia coli and Human Intestinal Cells

et al. 2020

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Lactic acid bacteria (LAB) “fermentates” confer a beneficial effect on intestinal function. However, the ability of new fermentations to improve LAB broth activity in preventing pathogen-induced intestinal inflammation and barrier dysfunction has not yet been studied. The objective of this study was to determine if broths of LAB fermented with Eruca sativa or Barbarea verna seed extracts prevent gut barrier dysfunction and interleukin-8 (CXCL8) release in vitro in human intestinal Caco-2 cells infected with enterohemorrhagic Escherichia coli (EHEC) O157:H7. LAB broths were assayed for their effects on EHEC growth and on Caco-2 viability; thereafter, their biological properties were analysed in a co-culture system consisting of EHEC and Caco-2 cells. Caco-2 cells infected with EHEC significantly increased CXCL8 release, and decreased Trans-Epithelial Electrical Resistance (TEER), a barrier-integrity marker. Notably, when Caco-2 cells were treated with LAB broth enriched with E. sativa seed extract and thereafter infected, both CXCL8 expression and epithelial dysfunction reduced compared to in untreated cells. These results underline the beneficial effect of broths from LAB fermented with E. sativa seed extracts in gut barrier and inflammation after EHEC infection and reveal that these LAB broths can be used as functional bioactive compounds to regulate intestinal function.

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Protective Effects of Lactic Acid Bacteria Against TLR4 Induced Inflammatory Response in Hepatoma HepG2 Cells Through Modulation of Toll-Like Receptor Negative Regulators of Mitogen-Activated Protein Kinase and NF-κB Signaling

Cited by 43 publications

References 50 publications

The protective effects of enriched citrulline fermented milk with Lactobacillus helveticus on the intestinal epithelium integrity against Escherichia coli infection

The protective effects of enriched citrulline fermented milk with Lactobacillus helveticus on the intestinal epithelium integrity against Escherichia coli infection

Beneficial effect of immunobiotic strains on attenuation of Salmonella induced inflammatory response in human intestinal epithelial cells

Effect of Lactobacillus acidophilus Fermented Broths Enriched with Eruca sativa Seed Extracts on Intestinal Barrier and Inflammation in a Co-Culture System of an Enterohemorrhagic Escherichia coli and Human Intestinal Cells

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