Protective Effects of <i>Ocimum gratissimum</i>Aqueous Extracts on HaCaT Cells Against UVC-Induced Inhibition of Cell Viability and Migration

Chang, Sheng-Huang; Liu, Jer-Yuh; Hsiao, Meen-Woon; Yang, Hsin‐Ling; Wang, Guan-Wei; Ye, Je-Chiuan

doi:10.7150/ijms.54644

Cited by 11 publications

(11 citation statements)

References 48 publications

(62 reference statements)

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“… Chang et al. (2021) reported that 100 μg/mL O. gratissimum restored cell activity and protected against ultraviolet C-induced inhibition of cell proliferation and migration of skin cells, and therefore can serve as a potent natural wound care agent.…”

Section: Pharmacological Activitiesmentioning

confidence: 99%

A review on the traditional uses, phytochemistry, and pharmacological activities of clove basil (Ocimum gratissimum L.)

Ugbogu

Emmanuel

Agi

et al. 2021

Heliyon

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In traditional medicine, Ocimum gratissimum (clove basil) is used in the treatment of various diseases such as diabetes, cancer, inflammation, anaemia, diarrhoea, pains, and fungal and bacterial infections. The present study reviewed the phytochemicals, essential oils, and pharmacological activities of O. gratissimum . The bioactive compounds extracted from O. gratissimum include phytochemicals (oleanolic acid, caffeic acid, ellagic acid, epicatechin, sinapic acid, rosmarinic acid, chlorogenic acid, luteolin, apigenin, nepetoidin, xanthomicrol, nevadensin, salvigenin, gallic acid, catechin, quercetin, rutin, and kaempfero) and essential oils (camphene, β-caryophyllene, α- and β-pinene, α-humulene, sabinene, β-myrcene, limonene, 1,8-cineole, trans-β-ocimene, linalool, α- and δ-terpineol, eugenol, α-copaene, β-elemene, p-cymene, thymol, and carvacrol). Various in vivo and in vitro studies have shown that O. gratissimum and its bioactive constituents possess pharmacological properties such as antioxidant, anti-inflammatory, anticancer, hepatoprotective, antidiabetic, antihypertensive, antidiarrhoeal, and antimicrobial properties. This review demonstrated that O. gratissimum has a strong preventive and therapeutic effect against several diseases. The effectiveness of O. gratissimum to ameliorate various diseases may be attributed to its antimicrobial and antioxidant properties as well as its capacity to improve the antioxidant systems. However, despite the widespread pharmacological activities of O. gratissimum , further experiments in human clinical trial studies are needed to establish effective and safe doses for the treatment of various diseases.

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Section: Pharmacological Activitiesmentioning

confidence: 99%

A review on the traditional uses, phytochemistry, and pharmacological activities of clove basil (Ocimum gratissimum L.)

Ugbogu

Emmanuel

Agi

et al. 2021

Heliyon

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“…There were some reports related to wound healing-related cell migration induced by herbal extracts, such as Ocimum gratissimum extract (17) and Coccinia grandis extract.…”

Section: Discussionmentioning

confidence: 99%

Coffea canephora Bean Extract Induces NIH3T3 Cell Migration

et al. 2021

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BACKGROUND: Wound healing is an essential biological process that consists of sequential steps aimed at restoring the architecture and function of damaged cells and tissues. There are empirical evidences of using pure coffee bean powder as an alternative medicine in treating various types of wounds. However, there is limited data on coffee-induced wound healing, especially migration of cells. Therefore, current study was conducted to investigate the role of coffee extract in cell migration, especially fibroblast which is important for wound healing.METHODS: Coffea canephora beans were prepared, extracted and added in the NIH3T3 cell culture in final concentration of 2.5% and 5%. Then cytotoxicity test was performed using Na,30-[(phenylamino)-carbonyl]-3,4-tetrazolium]-bis(4-methoxy-6-nitro) benzenesulfonic acid hydrate (XTT) assay. Meanwhile, cell migration test was performed with scratch assay. All results were statistically analyzed.RESULTS: The 2.5% or 5% Coffea canephora beans extract (CCBE)-treated NIH3T3 cell numbers were almost similar with the numbers of NIH3T3 cells in starvation medium merely. Meanwhile, 2.5% and 5% CCBE showed significant decrease of the widths of scratched areas compared to starvation medium merely (ANOVA with LSD Post-hoc, p=0.000). After 24 h and 48 h, the average widths of 2.5% and 5% CCBE-treated scratched areas were 235.68±22.79, 50.36±5.29, 229.95±23.01, 27.68±2.83, respectively.CONCLUSION: Since both 2.5% and 5% CCBE are potential in inducing migration of fibroblast (NIH3T3 cell) and do not induce cytotoxicity, the CCBE could be potential as an agent for wound healing.KEYWORDS: coffee, Coffea canephora, NIH3T3, migration, cytotoxicity

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“…Lipid peroxidation in the cell is a common detrimental outcome of free radicals (FRs) that end up decreasing the membrane potential and cell damage. Malondialdehyde (MDA) causes severe cell injury via stimulation of polymerization and cross linking in components of the membrane [5]. FRs react with DNA and produce 8-hydroxyguanine (8-OHGua) that is the destructive product of DNA [6].…”

Section: Introductionmentioning

confidence: 99%

Influence of Systemic Administration of Coq10 Nanoparticles on Ischemia-Reperfusion Injury on Ovaries in Rat

Honardoust

Najafpour

Rahim

2021

Evidence-Based Complementary and Alternative Medicine

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Using a rat ovary model, effects of COQ10 nanoparticles (NCOQ10) were studied on ischemia-reperfusion injury. In the present experimental study, following randomization of thirty healthy female Wistar rats ∼250 g, the animals were subjected to five experimental groups (n = 6): group SHAM : only laparotomy was performed, group IS: only a 3-hour ischemia was performed, group IS/REP: the procedure included a 3-hour ischemia followed by a 3-hour reperfusion, and 50 µL soybean oil (solvent of NCOQ10) was administered 30 min before cessation of reperfusion, group IS/NCOQ10: the procedure included a 3-hour ischemia only and 50 µL (0.3 mmol/lit/IP) of NCOQ10 30 min before cessation of ischemia, and group IS/REP/NCOQ10: the procedure included a 3-hour ischemia, a 3-hour reperfusion, and 20 µL (0.3 mmol/lit) of NCOQ10 30 min before cessation of ischemia. Significantly amended development of ischemia/reperfusion tissue injury was observed in animals treated with NCOQ10 compared to those of other groups ( P = 0.001 ). Mean values of biochemical indices were significantly higher than those observed for other groups ( P = 0.001 ). Significantly lower values of MDA were observed in IS/REP/NCOQ10 animals compared to those of other groups ( P = 0.001 ).Where ovarian tissue is exposed to ischemia, intraperitoneal administration of NCOQ10 could bear clinical benefits in diminishing ischemia-reperfusion injury.

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Protective Effects of Ocimum gratissimumAqueous Extracts on HaCaT Cells Against UVC-Induced Inhibition of Cell Viability and Migration

Cited by 11 publications

References 48 publications

A review on the traditional uses, phytochemistry, and pharmacological activities of clove basil (Ocimum gratissimum L.)

A review on the traditional uses, phytochemistry, and pharmacological activities of clove basil (Ocimum gratissimum L.)

Coffea canephora Bean Extract Induces NIH3T3 Cell Migration

Influence of Systemic Administration of Coq10 Nanoparticles on Ischemia-Reperfusion Injury on Ovaries in Rat

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