Protective effects of Cordyceps sinensis exopolysaccharide‑selenium nanoparticles on H2O2-induced oxidative stress in HepG2 cells

Xiao, Yidong; Zhang, Xiao; Huang, Qilin

doi:10.1016/j.ijbiomac.2022.05.173

Cited by 30 publications

(23 citation statements)

References 61 publications

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“…Se NPs have shown promise in cancer therapy by enhancing the therapeutic effect of anticancer agents while minimizing their adverse effects on normal cells. 56 The enhanced cytotoxicity of BSA-TAM NPs compared to BSA NPs is consistent with the known cytotoxic properties of TAM drug. TAM-loaded nanoparticles can improve drug delivery, cellular uptake, and cytotoxicity in cancer cells.…”

Section: Notesupporting

confidence: 73%

Selenium‐doped albumin nanoparticles enhance tamoxifen‐induced anticancer effects in 4T‐1 mouse breast cancer cells

Gharbavi,

Johari,

Tabar

et al. 2023

Applied Organom Chemis

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In this study, we aimed to improve the delivery and efficacy of the well‐known anticancer drug, tamoxifen (TAM), through its encapsulation in bovine serum albumin nanoparticles (BSA NPs) and their hybridization with selenium nanoparticles (Se NPs). The developed nanostructures were characterized by Fourier transform infrared (FT‐IR) spectroscopy, ultraviolet–visible (UV–Vis) spectroscopy, energy‐dispersive X‐ray spectroscopy (EDS) analysis, dynamic light scattering (DLS), and field emission scanning electron microscopy (FESEM) imaging. The impact of nanostructures on 4T‐1 cancer cells was assessed through MTT and flow cytometry assays to measure cytotoxicity and apoptosis rates, respectively. FT‐IR and UV–Vis spectroscopy confirmed the successful encapsulation of TAM within BSA NPs. EDS analysis confirmed the Se integration into BSA‐TAM@Se NPs, signifying successful hybridization. Size and zeta potential measurements revealed the average hydrodynamic size and surface charge of the nanostructures. BSA NPs, BSA@Se NPs, and Se NPs showed the sizes ranging from 153.37 ± 1.02 to 229.63 ± 1.36 nm, with zeta potential values from −10.12 ± 0.26 to −28.45 ± 0.40 mV. Hemocompatibility studies demonstrated minimal hemolytic activity of the nanostructures, indicating their biocompatibility for intravenous administration. Cellular toxicity assays showed higher cytotoxicity of BSA‐TAM@Se NPs compared to other formulations (****p < 0.0001). The increased cell death in 4T‐1 breast cancer cells highlights the enhanced therapeutic potential of BSA‐TAM@Se NPs. Apoptosis analysis revealed a significant increase in apoptosis rate in 4T‐1 cells treated with BSA‐TAM@Se NPs compared to other formulations (****p < 0.0001), emphasizing their ability to induce programmed cell death. These findings support the rational importance of BSA NPs as drug nanocarriers and their hybridization with Se NPs to improve therapeutic effects. Finally, we suggest that the developed BSA‐TAM@Se NPs can be used as a potential therapeutic approach in combination with other tools such as X‐ray irradiation in future studies.

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Section: Notesupporting

confidence: 73%

Selenium‐doped albumin nanoparticles enhance tamoxifen‐induced anticancer effects in 4T‐1 mouse breast cancer cells

Gharbavi,

Johari,

Tabar

et al. 2023

Applied Organom Chemis

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“…The reactive oxygen species (ROS) are also the perpetrators of bacterial growth suppression . SeNPs produce a high quantity of ROS, causing oxidative stress in the cell . The destruction of major cellular components such as proteins, DNA, and ribonucleic acid (RNA) caused by oxidative stress results in altered membrane permeability and increased biological component leakage from the cell .…”

Section: Resultsmentioning

confidence: 99%

“… 86 SeNPs produce a high quantity of ROS, causing oxidative stress in the cell. 87 The destruction of major cellular components such as proteins, DNA, and ribonucleic acid (RNA) caused by oxidative stress results in altered membrane permeability and increased biological component leakage from the cell. 88 The bacteria will suffer permanent oxidative damage and cell death as a result of this.…”

Section: Resultsmentioning

confidence: 99%

Green Orange Peel-Mediated Bioinspired Synthesis of Nanoselenium and Its Antibacterial Activity against Methicillin-Resistant Staphylococcus aureus

Dang‐Bao

Long

et al. 2022

ACS Omega

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In this study, green orange peel (GOP) was feasibly evidenced in preparing selenium nanoparticles (SeNPs). Acting as reducing agents, polyphenolic compounds were extracted from GOP at the optimal extraction conditions (at 70 °C for 1.5 h, mass ratio of dried orange peel/distilled water of 5/100). The formation of SeNPs was observed at the wavelength range of 250–300 nm by ultraviolet–visible spectroscopy (UV–vis), and their highest yield could be reached at the following conditions: volume ratio of extract/selenious acid solution (V Ext/V Se) of 40/10, synthesis duration of 4 h, selenious acid concentration (C Se) of 80 mM, and reaction temperature of 120 °C. The highly crystalline structure of SeNPs in the hexagonal phase was characterized by powder X-ray diffraction (XRD) with a lattice parameter of 4.3 Å; meanwhile, their spheres with an average crystal size of 18.3 nm were estimated by high-resolution transmission electron microscope (HR-TEM). The rationale of bioreducing agents extracted from green orange peel for the formation of SeNPs was also recognized by Fourier-transform infrared spectroscopy (FT-IR). The antibacterial investigation of the SeNP sample was assessed against antibiotic-resistant bacteria, typically methicillin-resistant Staphylococcus aureus (MRSA), by executing the zone of inhibition and the minimum inhibitory concentration (MIC) tests. The SeNP sample demonstrated excellent antibacterial activity with an average diameter of inhibition zones of 20.0 ± 0.7 mm and an MIC of 4.94 μg/L. A comparison of the physicochemical properties of SeNPs synthesized from GOP extract by the hydrothermal method with SeNP products from other green reducing agents and other methods as well as its antibacterial activity compared with other nanoparticles and some antibiotics was conducted to highlight the superiority of GOP-mediated green-synthesized SeNPs.

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“…At a dose level of 15 mg/kg, 1,3,4-oxadiazole derivative showed a significant ( P < 0.001) rise in the SOD level when compared to the diabetic group. Catalase performs its activity to convert H 2 O 2 into water and O 2 and protect the cell from oxidative stress [ 43 ]. However, unlike superoxide, H 2 O 2 can rapidly diffuse across cell membranes, and in the presence of transition metal ions, it can be converted to hydroxyl radicals via Fenton chemistry [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Antidiabetic Activity of Oxadiazole Derivative in Rats

Qazi

Ahmad

Sahibzada³

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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The oxadiazole ring has long been used for the treatment of several diseases. This study aimed to analyze the antihyperglycemic and antioxidant roles of the 1,3,4-oxadiazole derivative with its toxicity. Diabetes was induced through intraperitoneal administration of alloxan monohydrate at 150 mg/kg in rats. Glimepiride and acarbose were used as standards. Rats were divided into groups of normal control, disease control, standard, and diabetic rats (treated with 5, 10, and 15 mg/kg of 1,3,4-oxadiazole derivative). After 14 days of oral administration of 1,3,4-oxadiazole derivatives (5, 10, and 15 mg/kg) to the diabetic group, the blood glucose level, body weight, glycated hemoglobin (HbA1c), insulin level, antioxidant effect, and histopathology of the pancreas were performed. The toxicity was measured by estimating liver enzyme, renal function, lipid profile, antioxidative effect, and liver and kidney histopathological study. The blood glucose and body weight were measured before and after treatment. Alloxan significantly increased blood glucose levels, HbA1c, alanine transaminase, aspartate aminotransferase, urea, cholesterol, triglycerides, and creatinine. In contrast, body weight, insulin level, and antioxidant factors were reduced compared to the normal control group. Treatment with oxadiazole derivatives showed a significant reduction in blood glucose levels, HbA1c, alanine transaminase, aspartate aminotransferase, urea, cholesterol, triglycerides, and creatinine as compared to the disease control group. The 1,3,4-oxadiazole derivative significantly improved body weight, insulin level, and antioxidant factors compared to the disease control group. In conclusion, the oxadiazole derivative showed potential antidiabetic activity and indicated its potential as a therapeutic.

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Protective effects of Cordyceps sinensis exopolysaccharide‑selenium nanoparticles on H2O2-induced oxidative stress in HepG2 cells

Cited by 30 publications

References 61 publications

Selenium‐doped albumin nanoparticles enhance tamoxifen‐induced anticancer effects in 4T‐1 mouse breast cancer cells

Selenium‐doped albumin nanoparticles enhance tamoxifen‐induced anticancer effects in 4T‐1 mouse breast cancer cells

Green Orange Peel-Mediated Bioinspired Synthesis of Nanoselenium and Its Antibacterial Activity against Methicillin-Resistant Staphylococcus aureus

Evaluation of Antidiabetic Activity of Oxadiazole Derivative in Rats

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