Protective Effects of Boysenberry Anthocyanins on Oxidative Stress in Diabetic Rats

Abstract

The protective effects of major boysenberry anthocyanins (BoAnt) against oxidative stress were investigated in streptozotocin (STZ)-induced diabetic rats. Increases in the concentration of plasma thiobarbituric acid reactive substances (TBARS), and in the liver 8-hydroxy deoxyguanosine (8-OH dG)/deoxyguanosine (dG) ratio and also in the liver GSSG/GSH ratio, which were all observed in STZ-injected rats, were restored or tended to be restored to the level of the control rats when a diet with BoAnt was given to the diabetic animals. The susceptibility of the liver homogenate of the diabetic rats to the oxidation by AAPH was relieved when BoAnt was fed to them. These results suggested that BoAnt was effective in protecting the development of in vivo oxidation involved with diabetes.

Evaluation of the effects of anthocyanins in type 2 diabetes

“…Therefore, the inhibition or reduction of oxidative processes, as additional therapy, can prevent or delay the onset of complications associated with diabetes (Bonina et al, 2002). This prevention can be accomplished with the use of antioxidants and also through the increased expression of antioxidant enzymes; the administration of ANT can be effective in such circumstances (Feshani et al, 2011;Sugimoto et al, 2003).…”

“…Several experiments have demonstrated the antidiabetic properties of ANTs, which are attributed to multiple and simultaneous effects of these bioactive compounds, including reducing blood glucose, glucosuria and Hb A1c; preventing free radical production; increasing insulin secretion; and improving insulin resistance (Ataie-Jafari, Hosseini, Karimi, & Pajouhi, 2008;Grace et al, 2009;Guo et al, 2007;Iwai, Onodera, & Matsue, 2004;Jayaprakasam, Vareed, Olson, & Nair, 2005;Roy et al, 2008;Sugimoto, Igarachi However, some studies with healthy, non-diabetic humans revealed that ANT did not alter blood glucose or plasma insulin concentrations (Basu et al, 2009;Chambers & Camire, 2003;Kelley et al, 2006), perhaps because it requires the presence of pre-existing metabolic disorders for which these compounds have an effective action.…”

“…A number of experiments in animal models indicate that the elevation of thiobarbituric acid reactive substances (TBARS), of MDA and oxidized glutathione as well as decreased concentrations of SOD and catalase are indicators of lipid oxidation and antioxidant enzymes in the diabetic state (Guo et al, 2007;Roy et al, 2008;Sugimoto et al, 2003).…”

Comparison of the Preventive Activity of Isorhamnetin Glycosides from Atsumi-kabu (Red Turnip,Brassica, campestrisL.) Leaves on Carbon Tetrachloride-Induced Liver Injury in Mice

“…The mice were kept under controlled conditions with a 12-h light and 12-h dark cycle (6:00-18:00 light) in a temperature range of [22][23][24] C and a relative humidity of 40-60%.…”

“…The tricloromethyl radical produced from carbon tetrachloride by its dechlorination from the action of cytochrome P450 isozymes is thought to induce the formation of the lipid radical and consecutive lipid peroxidation to finally cause liver cell damage. 15) Leaves of atsumi-kabu (2.7 kg dry weight), which had been dried in a room at about [20][21][22][23][24][25] C with ventilation, were extracted with methyl alcohol (MeOH) under reflux. The evaporated extract was dissolved in MeOH and mixed with chloroform and distilled water in the ratio of 0.5:1:2 (v/v/v).…”