Protective Effects of Baicalin and Octreotide on Multiple Organ Injury in Severe Acute Pancreatitis

Zhang, Xi Ping; Zhang, Ling; Yang, Ping; Zhang, Rui Ping; Cheng, Qi

doi:10.1007/s10620-007-9868-3

Cited by 42 publications

(61 citation statements)

References 24 publications

(16 reference statements)

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“…Fix them according to the related requirements, observe the pathological changes of pancreas after HE staining and compare the pathological scores among groups. The standard of pancreas pathological score was in accordance with reference [2] . The content of amylase, endotoxin in plasma, and TNF-α, IL-6 and PLA2 in serum of all groups were determined at different time points.…”

Section: Observation Indexesmentioning

confidence: 99%

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Effects of large dose of dexamethasone on inflammatory mediators and pancreatic cell apoptosis of rats with severe acute pancreatitis

Zhang

Chen²,

Hu³

et al. 2007

WJG

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AIM:To investigate the influence of high dose of dexamethasone on inflammatory mediators and apoptosis of rats with severe acute pancreatitis (SAP).METHODS: SAP rats were randomly assigned to the model group and treatment group while the normal rats were assigned to the sham operation group. The mortality, ascite volumes, ascites/body weight ratio and pancreas pathological changes of all rats were observed at 3, 6 and 12 h after operation. Their contents of amylase and endotoxin in plasma and contents of tumor necrosis factor (TNF-α), phospholipase A2 (PLA2) and IL-6 in serum were also determined. The microarray sections of their pancreatic tissues were prepared, terminal transferase dUTP nick end labeling (TUNEL) staining was performed and apoptotic indexes were calculated. RESULTS:There was no marked difference between treatment group and model group in survival. The contents of amylase and endotoxin in plasma and contents of TNF-α, PLA2 and IL-6 in serum, ascite volumes, ascites/body weight ratio and pancreas pathological scores were all lower in treatment group than in model group to different extents at different time

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Section: Observation Indexesmentioning

confidence: 99%

“…Two chief pathologists used the blind method for scoring [2] . Gross pathological changes of pancreas.…”

Section: Comparison Of Serum Pla2 Contentmentioning

confidence: 99%

Effects of large dose of dexamethasone on inflammatory mediators and pancreatic cell apoptosis of rats with severe acute pancreatitis

Zhang

Chen²,

Hu³

et al. 2007

WJG

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“…Several previous studies demonstrated that baicalin attenuated lung edema in the rats model of air embolism-induced acute lung injury, multiple organ injury in sever acute pancreatitis and sepsis induced by cecal ligation and puncture (Ding et al 2016;Li et al 2009;Zhang et al 2008). Our results showed that baicalin also attenuated lung edema in LPS-induced acute lung injury.…”

Section: Discussionmentioning

confidence: 97%

“…Recent studies indicate that baicalin exerts a multitude of pharmacological activities including inhibition of platelet aggregation (Lee et al 2015), reduction of endotoxin generation (Liu et al 2008) and bacteria resistance (Liu et al 2000). Zhang et al 2008 have shown that baicalin improved tissue injury in experimental sepsis and multiple organ injury in severe pancreatitis. Moreover, baicalin attenuated air embolism-induced ALI and inhibited inflammatory effects against lipopolysaccharide (LPS)-induced ALI (Ding et al 2016;Li et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Effects of baicalin on alveolar fluid clearance and α-ENaC expression in rats with LPS-induced acute lung injury

Deng

Wang

Liang

et al. 2017

Can. J. Physiol. Pharmacol.

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Baicalin has been reported to attenuate lung edema in the process of lung injury. However, the effect of baicalin on alveolar fluid clearance (AFC) and epithelial sodium channel (ENaC) expression has not been tested. Sprague-Dawley rats were anesthetized and intratracheally injected with either 1 mg/kg lipopolysaccharide (LPS) or saline vehicle. Baicalin with various concentrations (10, 50, and 100 mg/kg) was injected intraperitoneally 30 min before administration of LPS. Then lungs were isolated for measurement of AFC, cyclic adenosine monophosphate (cAMP) level, and cellular localization of α-ENaC. Moreover, mouse alveolar type II (ATII) epithelial cell line was incubated with baicalin (30 μmol/L), adenylate cyclase inhibitor SQ22536 (10 μmol/L), or cAMP-dependent protein kinase inhibitor (PKA) KT5720 (0.3 μmol/L) 15 min before LPS (1 μg/mL) incubation. Protein expression of α-ENaC was detected by Western blot. Baicalin increased cAMP concentration and AFC in a dose-dependent manner in rats with LPS-induced acute lung injury. The increase of AFC induced by baicalin was associated with an increase in the abundance of α-ENaC protein. SQ22536 and KT5720 prevented the increase of α-ENaC expression caused by baicalin in vitro. These findings suggest that baicalin prevents LPS-induced reduction of AFC by upregulating α-ENaC protein expression, which is activated by stimulating cAMP/PKA signaling pathway.

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“…The baicalin is a kind of flavone compounds, has significant biological activity, antibacterial, diuretic, anti-inflammatory, anti-allergic and antispasmodic effects, and has strong antitumor responses and other physiological efficiency. 3,4 The phillyrin has the effects of clearing heat, detoxification, attending warm, boils, scrofula, erysipelas, measles, influenza class. 5,6 Their chemical structures and molecular weights as shown in the Fig.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination Baicalin and Forsythin in Shuanghuanglian Oral Liquid by HPLC/DAD and LC‐ESI‐MS

Yan

et al. 2013

J Chinese Chemical Soc

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Rapid, simple and reliable HPLC/DAD and LC-ESI-MS methods for the simultaneous determination of baicalin and forsythin in the traditional Chinese medicinal preparation Shuanghuanglian oral liquid were described and validated. The separation condition for HPLC/DAD was optimized using a BDS hypersil C18 column (Thermo, 2.1 × 150 mm, particle size 5 µm) by gradient elution using methanol-0. + for forsythin, respectively. These HPLC/DAD and LC-ESI-MS methods were validated in terms of recovery, linearity, accuracy and precision (intra-and inter-day validation). These methods can be used as a complementary method for the commercial quality control of Shuanghuanglian oral liquid and its pharmaceutical preparations.

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Protective Effects of Baicalin and Octreotide on Multiple Organ Injury in Severe Acute Pancreatitis

Cited by 42 publications

References 24 publications

Effects of large dose of dexamethasone on inflammatory mediators and pancreatic cell apoptosis of rats with severe acute pancreatitis

Effects of large dose of dexamethasone on inflammatory mediators and pancreatic cell apoptosis of rats with severe acute pancreatitis

Effects of baicalin on alveolar fluid clearance and α-ENaC expression in rats with LPS-induced acute lung injury

Simultaneous Determination Baicalin and Forsythin in Shuanghuanglian Oral Liquid by HPLC/DAD and LC‐ESI‐MS

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