Effects of large dose of dexamethasone on inflammatory mediators and pancreatic cell apoptosis of rats with severe acute pancreatitis

Zhang, Xiping; Chen, Li; Hu, Qi-Fang; Tian, Hua; Xu, Ru-Jun; Wang, Zhiwei; Wang, Keyi; Cheng, Qi; Yan, Wei; Li, Yun; Li, Qingyu; He, Qing; Wang, Fei

doi:10.3748/wjg.v13.i41.5506

Cited by 39 publications

(41 citation statements)

References 25 publications

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“…A previous study demonstrated that pancreatic cell apoptosis occurs during acute pancreatitis (34). Animal experiments also suggested that dexamethasone induces pancreatic cell apoptosis, stabilizes the internal environment and attenuates inflammation in pancreatic tissues (33,35). In the present study, dexamethasone enhanced the phagocytotic capacity of pMФs, maintained the internal environment and increased MФ ICAM-3 expression.…”

Section: Discussionsupporting

confidence: 51%

“…The effects of glucocorticoids on inflammation via receptor mediation may trigger anti-inflammatory processes. Glucocorticoids inhibit inflammatory exudation, leukocytic infiltration and inflammatory mediator production and release, improve the microcirculation, alleviate endotoxemia and induce apoptosis of pancreatic acinar cells, thereby reducing the degree of pancreatic necrosis in SAP (32,33). A previous study demonstrated that pancreatic cell apoptosis occurs during acute pancreatitis (34).…”

Section: Discussionmentioning

confidence: 99%

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In vitro effects of emodin on peritoneal macrophage intercellular adhesion molecule-3 in a rat model of severe acute pancreatitis/systemic inflammatory response syndrome

Zhang

Sun

et al. 2013

Biomedical Reports

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Abstract. Rhubarb is often used in Chinese herbal medicine for the treatment of systemic inflammatory response syndrome (SIRS). Emodin is the main active constituent of rhubarb. This study was performed to investigate the in vitro effects of emodin and dexamethasone on peritoneal macrophage (pMΦ) phagocytosis and the expression of intercellular adhesion molecule-3 (ICAM-3). A total of 40 Sprague-Dawley (SD) rats were randomly divided into sham surgery (n=10) and model groups (n=30). After 24 h, pMΦs were harvested and the model group was randomly divided into three subgroups (n=10 rats̸subgroup): the 5 µg/ml emodin, 0.1 µmol/ml dexamethasone and control groups. The drugs were administered following macrophage (MΦ) adhesion for 24 h. pMΦ phagocytosis was significantly increased in the emodin group compared to that in the control group. Moreover, pMΦ phagocytosis was significantly increased in the emodin group compared to that in the dexamethasone group. The expression of ICAM-3 was significantly increased in the emodin group compared to that in the control group. The expression of ICAM-3 was significantly increased in the emodin group compared to that in the dexamethasone group. The expression of ICAM-3 was significantly increased in the emodin and dexamethasone groups compared to that in the control group. pMΦ phagocytosis and ICAM-3 expression were significantly increased following emodin treatment compared to those in the control and dexamethasone groups, indicating that emodin may enhance pMΦ phagocytosis and apoptotic cell clearance by altering ICAM-3 expression.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

In vitro effects of emodin on peritoneal macrophage intercellular adhesion molecule-3 in a rat model of severe acute pancreatitis/systemic inflammatory response syndrome

Zhang

Sun

et al. 2013

Biomedical Reports

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“…After inserting a segmental epidural catheter into the duodenum cavity via the hole, it was inserted into the biliary-pancreatic duct, in the direction of the papilla, in a retrograde position. This was followed by retrograde transfusion of 3.5% sodium taurocholate 0.1 mL/100 g by microinjection pump at the speed of 0.2 mL/min, and then the hole in the duodenum lateral wall was sutured [2][3][4][5][6] .…”

Section: Animal Grouping and Rat Sap Model Preparationmentioning

confidence: 99%

Influence of dexamethasone on mesenteric lymph node of rats with severe acute pancreatitis

Zhang¹,

Xu²,

Jiang³

et al. 2008

WJG

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AIM:To study the influence and mechanisms of dexamethasone on mesenteric lymph node of rats with severe acute pancreatitis (SAP). METHODS:The SAP rats were assigned to model, treated or sham-operated groups. The mortality, pathological changes of mesenteric lymph nodes, expression levels of NF-kB, P-selectin, Bax, Bcl-2 and caspase-3 protein and changes in apoptotic indexes in lymph nodes were observed at 3, 6 and 12 h after operation. The blood levels of endotoxin, superoxide dismutase (SOD), malondialdehyde (MDA), and endothelin-1 (ET-1) in blood were determined. RESULTS: SOD content, expression of Bax protein and apoptotic index were significantly higher in the treated group than in the model group at different time points (P < 0.05 or P < 0.01). Other blood-detecting indexes and histopathological scores of mesenteric lymph nodes were lower in the treated than in the model group (P < 0.05, P < 0.01 or P < 0.01). NF-kB protein expression was negative in all groups. Comparing P-selectin and caspase-3 expression levels among all three groups, there was no marked difference between the model and treated group. CONCLUSION: Dexamethasone can protect mesenteric lymph nodes. The mechanism may be by reducing the content of inflammatory mediators in the blood and inducing lymphocyte apoptosis.

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“…Salvia miltiorrhiza ('Danshen' in Chinese) injection and dexamethasone injection are representative traditional Chinese and Western medicines, respectively, most frequently used for the treatment of SAP patients. Salvia miltiorrhiza exerts therapeutic effects on SAP mainly by improving microcirculation, while dexamethasone acts mainly through suppressing inflammatory mediators (Zhang et al, 2007d;2007e). Salvia miltiorrhiza is a commonly used Chinese herb for promoting blood flow and removing blood stasis.…”

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confidence: 99%

Effects of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis

Zhang

Chengjun

et al. 2012

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To investigate the protective effects and mechanisms of action of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis (SAP). Methods: The rats were divided into sham-operated, model control, dexamethasone treated, and Salvia miltiorrhiza treated groups. At 3, 6, and 12 h after operation, the mortality rate of different groups, pathological changes, Bcl-2-associated X protein (Bax) and nuclear factor-κB (NF-κB) protein expression levels in multiple organs (the pancreas, liver, kidneys, and lungs), toll-like receptor 4 (TLR-4) protein levels (only in the liver), intercellular adhesion molecule 1 (ICAM-1) protein levels (only in the lung), and terminal deoxynucleotidy transferase mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) staining expression levels, as well as the serum contents of amylase, glutamate-pyruvate transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), blood urea nitrogen (BUN), and creatinine (CREA) were observed. Results: The mortality rate of the dexamethasone treated group was significantly lower than that of the model control group (P<0.05). The pathological changes in multiple organs in the two treated groups were relieved to different degrees (P<0.05 and P<0.01, respectively), the expression levels of Bax and NF-κB proteins, and apoptotic indexes of multiple organs were reduced (P<0.05 and P<0.01, respectively). The contents of amylase, GPT, GOT, BUN, and CREA in the two treated groups were significantly lower than those in model control groups (P<0.05 and P<0.01, respectively). The expression level of ICAM-1 protein in the lungs (at 3 and 12 h) in the dexamethasone treated group was significantly lower than that in the Salvia miltiorrhiza treated group (P<0.05). The serum contents of CREA (at 12 h) and BUN (at 6 h) of the Salvia miltiorrhiza treated group were significantly lower than those in the dexamethasone treated group (P<0.05). Conclusions: Both dexamethasone and Salvia miltiorrhiza can reduce the inflammatory reaction, regulate apoptosis, and thus protect multiple organs of rats with SAP.

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Effects of large dose of dexamethasone on inflammatory mediators and pancreatic cell apoptosis of rats with severe acute pancreatitis

Cited by 39 publications

References 25 publications

In vitro effects of emodin on peritoneal macrophage intercellular adhesion molecule-3 in a rat model of severe acute pancreatitis/systemic inflammatory response syndrome

In vitro effects of emodin on peritoneal macrophage intercellular adhesion molecule-3 in a rat model of severe acute pancreatitis/systemic inflammatory response syndrome

Influence of dexamethasone on mesenteric lymph node of rats with severe acute pancreatitis

Effects of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis

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