Influence of dexamethasone on mesenteric lymph node of rats with severe acute pancreatitis

Abstract

AIM:To study the influence and mechanisms of dexamethasone on mesenteric lymph node of rats with severe acute pancreatitis (SAP). METHODS:The SAP rats were assigned to model, treated or sham-operated groups. The mortality, pathological changes of mesenteric lymph nodes, expression levels of NF-kB, P-selectin, Bax, Bcl-2 and caspase-3 protein and changes in apoptotic indexes in lymph nodes were observed at 3, 6 and 12 h after operation. The blood levels of endotoxin, superoxide dismutase (SOD), malondialdehyde (MDA), and endothelin-1 (ET-1) in blood were determined. RESULTS: SOD content, expression of Bax protein and apoptotic index were significantly higher in the treated group than in the model group at different time points (P < 0.05 or P < 0.01). Other blood-detecting indexes and histopathological scores of mesenteric lymph nodes were lower in the treated than in the model group (P < 0.05, P < 0.01 or P < 0.01). NF-kB protein expression was negative in all groups. Comparing P-selectin and caspase-3 expression levels among all three groups, there was no marked difference between the model and treated group. CONCLUSION: Dexamethasone can protect mesenteric lymph nodes. The mechanism may be by reducing the content of inflammatory mediators in the blood and inducing lymphocyte apoptosis.

Effects of Baicalin and Octreotide on the Serum TNF-α Level and Apoptosis in Multiple Organs of Rats with Severe Acute Pancreatitis

“…In addition, apoptosis also occurs in extrapancreatic organs such as small intestine and lung, etc., and the extent of apoptosis is closely associated with the severity of multiple organ injury [26][27][28]. Some researchers believed that apoptosis was detrimental in SAP [29][30][31][32] while the others thought that apoptosis might provide some protective effects in SAP process [18,20,33,34]. The results of this study support the latter view.…”

“…1 and 2), Bax immunostaining and TUNEL staining were conducted, respectively. The pathological severity score standard for pancreas, lung and lymph nodes as well as the methods for Bax immunostaining and TUNEL staining were same as those described in our series of reports [16][17][18][19][20] Table 1.…”

Efficacy and Mechanism of Salvia Miltiorrhizae Injection in the Treatment of Rats with Severe Acute Pancreatitis

“…In order to improve the therapeutic effect of SAP, seeking cheap and effective drugs with fewer side effects has always been a hot spot during clinical studies of this disease [18][19][20][21]. The raw material of Salvia miltiorrhizae injection is the root of wild Salvia miltiorrhizae.…”

Influence of dexamethasone on the expression levels of P-selectin protein in multiple organs of rats with severe acute pancreatitis

“…For the sham-operated group, laparotomy was performed, the pancreas and duodenum were turned over, and the abdomen was closed. The sham-operated and model control groups were injected with saline of the same volume via the vena caudalis 15 min after the operation [17][18][19][20].…”