Protective effects of asiaticoside on septic lung injury in mice

Zhang, Lina; Ji, Zheng; Zhang, Li; Gong, Xia; Huang, Hai; Wang, Chang-dong; Wang, Bin; Wu, Mengjiao; Li, Xiaohui; Sun, Wen-Ji; Ying-ju, Liu; Wan, Jingyuan

doi:10.1016/j.etp.2010.04.002

Cited by 38 publications

(30 citation statements)

References 47 publications

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“…In mouse CLP model, Cox-2 expression was previously shown to increase in the lungs of septic mice [66], [67], [68], in addition dual inhibition of Cox-2 and 5-LOX successfully attenuated lung injury, reduced MPO activity and improved survival of these mice [69]. As shown in several in vitro and in vivo models, pathogens induce Cox-2 expression via activated Toll like receptors (TLRs) [39], [40].…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus rhamnosus GG and Bifidobacterium longum Attenuate Lung Injury and Inflammatory Response in Experimental Sepsis

et al. 2014

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IntroductionProbiotic use to prevent nosocomial gastrointestinal and potentially respiratory tract infections in critical care has shown great promise in recent clinical trials of adult and pediatric patients. Despite well-documented benefits of probiotic use in intestinal disorders, the potential for probiotic treatment to reduce lung injury following infection and shock has not been well explored.ObjectiveEvaluate if Lactobacillus rhamnosus GG (LGG) or Bifidobacterium longum (BL) treatment in a weanling mouse model of cecal ligation and puncture (CLP) peritonitis will protect against lung injury.Methods3 week-old FVB/N mice were orally gavaged with 200 µl of either LGG, BL or sterile water (vehicle) immediately prior to CLP. Mice were euthanized at 24 h. Lung injury was evaluated via histology and lung neutrophil infiltration was evaluated by myeloperoxidase (MPO) staining. mRNA levels of IL-6, TNF-α, MyD88, TLR-4, TLR-2, NFΚB (p50/p105) and Cox-2 in the lung analyzed via real-time PCR. TNF-α and IL-6 in lung was analyzed via ELISA.ResultsLGG and BL treatment significantly improved lung injury following experimental infection and sepsis and lung neutrophil infiltration was significantly lower than in untreated septic mice. Lung mRNA and protein levels of IL-6 and TNF-α and gene expression of Cox-2 were also significantly reduced in mice receiving LGG or BL treatment. Gene expression of TLR-2, MyD88 and NFΚB (p50/p105) was significantly increased in septic mice compared to shams and decreased in the lung of mice receiving LGG or BL while TLR-4 levels remained unchanged.ConclusionsTreatment with LGG and BL can reduce lung injury following experimental infection and sepsis and is associated with reduced lung inflammatory cell infiltrate and decreased markers of lung inflammatory response. Probiotic therapy may be a promising intervention to improve clinical lung injury following systemic infection and sepsis.

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Section: Discussionmentioning

confidence: 99%

Lactobacillus rhamnosus GG and Bifidobacterium longum Attenuate Lung Injury and Inflammatory Response in Experimental Sepsis

et al. 2014

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“…It has been reported that asiaticoside exhibits anti-oxidative, anti-inflammatory, antidepressant, wound healing and hepato-protective properties [24][25][26][27][28]. Moreover, asiaticoside could attenuate diabetic microangiopathy and accelerate nerve regeneration [29][30].…”

Section: Introductionmentioning

confidence: 99%

Asiaticoside attenuates diabetes-induced cognition deficits by regulating PI3K/Akt/NF-κB pathway

Yin

Chen

et al. 2015

Behavioural Brain Research

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“…The recoveries were 100. 5 procedure could be carried out with 30 min including SPE-clean-up and HPLC procedures, which is much lower than reported research [13]. The limits of quantitation of all tested compounds were lower than reported article [12].…”

Section: Recoverymentioning

confidence: 68%

“…Asiaticoside is effective in wound healing [1,2], burn wound repair [3], lung injury of mice [4,5] and antidepressant-like effect [6]. Asiaticoside is clinically used as an agent for psoriasis, wound healing, ulceration and eczema in combination with madecassic acid and asiatic acid [7].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of asiaticoside and its novel metabolites in culture of Aspergilus niger by SPE-HPLC

Chang

Wang²

2011

2011 International Conference on Remote Sensing, Environment and Transportation Engineering

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Abstracts-When cultured withAspergillus niger, asiaticoside, purified from Centella Asiatica (L.) Urban, was metabolized into four metabolites including one novel alkaloid. A simple and rapid SPE-HPLC method for simultaneous determination of asiaticoside and its four metabolites in culture of A. niger was developed. The chromatographic separation was achieved on a Dikma Diamonsi C 18 ODS column (200×4.6 mm i.d.) by gradient elution with 0.1% phosphoric acid in water and 0.1% phosphoric acid in methanol as the gradient mixtures. The flow rate was 1 mL/min, the detection wavelength was 204 nm and the column temperature was kept at 28 . The retention times of asiaticoside, asiatic acid, M 1 , M 2 , and M 3 were 11.3, 9.4 and 17.1, 19.3 and 7.9 min, respectively. The mean recoveries of five analysts were all over 99%. Quantification limits were 0.02 g/mL for all tested compounds. The method was sucessively applied to the quantification of the biotransformation of asiaticoside. Asiaticoside is one of abundant triterpene glycoside in Centella Asiatica (L.) Urban. Asiaticoside is effective in wound healing [1, 2], burn wound repair [3], lung injury of mice[4, 5] and antidepressant-like effect [6]. Asiaticoside is clinically used as an agent for psoriasis, wound healing, ulceration and eczema in combination with madecassic acid and asiatic acid [7]. Wound and ulcer healing of asiaticoside are enhanced by the promotion of fibroblast proliferation and collagen synthesis [8]. Our previous research showed that, asiaticoside was biotransformed into two aglycones, asiatic acid and one novel alkaloid after 5 days of cultured with Aspgergillus niger (Figure 1). The determination of asiaticoside in Centella Asiatica by HPLC [9,10] and rat plasma by LC-MS-MS [11] and by HPLC [12] had beenreported. But the HPLC combined with solid-phase extraction (SPE) method for simultaneous determination of asiaticoside and its metabolisms, especially the novel alkaloid, has not been reported. In this paper, one simple and rapid SPE-HPLC method was developed and successively applied to quantity the production of metabolisms in the culture of Aspgergillus niger during the biotransformation.

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Protective effects of asiaticoside on septic lung injury in mice

Cited by 38 publications

References 47 publications

Lactobacillus rhamnosus GG and Bifidobacterium longum Attenuate Lung Injury and Inflammatory Response in Experimental Sepsis

Lactobacillus rhamnosus GG and Bifidobacterium longum Attenuate Lung Injury and Inflammatory Response in Experimental Sepsis

Asiaticoside attenuates diabetes-induced cognition deficits by regulating PI3K/Akt/NF-κB pathway

Simultaneous determination of asiaticoside and its novel metabolites in culture of Aspergilus niger by SPE-HPLC

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