Protective Effects of Anthocyanins from Coreopsis tinctoria against Oxidative Stress Induced by Hydrogen Peroxide in MIN6 Cells

Abstract

Anthocyanins (AC) from Coreopsis tinctoria possesses strong antioxidant properties, while the effects of AC on cells damage induced by reactive oxygen species (ROS) in diabetes mellitus diseases progression have not been reported. The present study was carried out to evaluate the protective property of AC against cellular oxidative stress with an experimental model, H2O2‐exposed MIN6 cells. AC could reverse the decrease of cell viability induced by H2O2 and efficiently suppressed cellular ROS production and cell apoptosis. In addition, Real‐time PCR and Western blot analyses indicated that AC could protect MIN6 cells against oxidative injury through increasing the translocation of Nrf2 into nuclear, decreasing the phosphorylation level of p38 and up‐regulating the protein expression of antioxidant enzyme (SOD1, SOD2 and CAT). Thus, this study provides evidence to support the beneficial effect of AC in inhibiting MIN6 cells from H2O2‐induced oxidative injury.

IMT504 protects beta cells against apoptosis and maintains beta cell identity, without modifying proliferation

“…H 2 O 2 is a well‐known trigger of apoptosis through oxidative stress involving the mitochondrial pathway (Xiang et al, 2016 ). Moreover, H 2 O 2 has been shown to induce MIN6B1 cell death by apoptosis (Liu et al, 2020 ). Treatment with a low concentration of H 2 O 2 (0.75 μM) was found to be sufficient to induce significant apoptosis in MIN6B1 cells (Figure 5a ).…”

The Neuroprotective Effects of Coreopsis tinctoria and Its Mechanism: Interpretation of Network Pharmacological and Experimental Data

“…(CT), an annual herb in the genus Coreopsis, is an important traditional medicine in North America, Europe, and Asia for a long historical period (Mu et al, 2020;Shen et al, 2021). CT has a variety of pharmacological activities, such as antihyperglycemic, antihyperlipidemic, antihypertensive, antioxidant (Liu et al, 2020), anti-inflammatory (Yao et al, 2019), and hepatoprotective (Tsai et al, 2017;Tian et al, 2018). The antidiabetic effect of CT is widely acknowledged due to its obvious regulation of blood glucose.…”