Protective effects of amifostine on ischemia-reperfusion injury of rat kidneys

Merter, Ayse Arducoglu; Mayır, Burhan; Erdoğan, Okan; Çolak, Taner

doi:10.4103/0253-7613.153427

Cited by 8 publications

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First, we evaluated whether EGCG could alleviate early renal injury after burn in rats from two aspects: histopathology and functional indicators. According to previous literature, researchers have also discovered that AKI can lead to tubular necrosis, which subsequently impairs renal function [ 27 , 28 ]. The experiment showed that EGCG treatment significantly attenuated the renal tubule injury scores of burned rats, indicating its ability to alleviate structural damage in burned renal tissue.…”

Section: Discussionmentioning

confidence: 99%

Alleviation effects of epigallocatechin-3-gallate against acute kidney injury following severe burns

Xu,

Pang,

Sun

et al. 2023

Clin Exp Nephrol

View full text Add to dashboard Cite

Background Burn patients often face a high risk of acute kidney injury (AKI) after severe burn injuries, meanwhile epigallocatechin-3-gallate (EGCG) has been proven to be effective in alleviating organ injury. Methods This study used the classical burn model in rats. Thirty model rats were randomly divided into a Burn group, a Burn + placebo group, a Burn + EGCG (50 mg/kg) group, and ten non-model rats as Sham group. The urinary excretion of the rats was subsequently monitored for a period of 48 h. After 48 h of different treatments, rat serum and kidneys were taken for the further verification. The efficacy of EGCG was assessed in pathological sections, biochemical indexes, and at the molecular level. Results Pathological sections were compared between the Burn group and Burn + placebo group. The rats in the Burn + EGCG group had less kidney damage. Moreover, the EGCG group maintained significantly elevated urine volumes, biochemical indexes manifested that EGCG could reduce serum creatinine (Cr) and neutrophil gelatinase-associated lipocalin (NGAL) level and inhibit the oxidation-related enzyme malondialdehyde (MDA) level, meanwhile the superoxide dismutase (SOD) level was increased. The molecular level showed that EGCG significantly reduced the mRNA expression levels of the inflammation-related molecules interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Conclusion The research indicated that EGCG had an alleviating effect on kidney injury in severely burned rats, and its alleviating effects were related to improving kidney functions, alleviating oxidative stress, and inhibiting the expression of inflammatory factors.

show abstract

Section: Discussionmentioning

confidence: 99%

Alleviation effects of epigallocatechin-3-gallate against acute kidney injury following severe burns

Xu,

Pang,

Sun

et al. 2023

Clin Exp Nephrol

View full text Add to dashboard Cite

show abstract

“…At present, amifostine is mainly used in reducing the side effects induced by radiation therapy in the patients with malignant tumors [ 27 ]. In an in vivo study, Merter et al found that amifostine could attenuate ischemia/reperfusion injury of monkey kidneys by increasing intracellular GSH level [ 28 ]. In rat immature hippocampal neurons exposed to γ -rays, amifostine protects the neurons against the irradiation injury [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

SOD2 Mediates Amifostine‐Induced Protection against Glutamate in PC12 Cells

Jia

Zhang

Shi

et al. 2015

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Background. Cytoprotectant amifostine attenuates radiation-induced oxidative injury by increasing intracellular manganese superoxide dismutase (SOD2) in peripheral tissue. However, whether amifostine could protect neuronal cells against oxidative injury has not been reported. The purpose of this study is to explore the protection of amifostine in PC12 cells. Methods. PC12 cells exposed to glutamate were used to mimic neuronal oxidative injury. SOD assay kit was taken to evaluate intracellular Cu/Zn SOD (SOD1) and SOD2 activities; western blot analysis and immunofluorescence staining were performed to investigate SOD2 protein expression; MTT, lactate dehydrogenase (LDH), release and cell morphology were used to evaluate cell injury degree, and apoptotic rate and cleaved caspase-3 expression were taken to assess apoptosis; mitochondrial superoxide production, intracellular reactive oxygen species (ROS), and glutathione (GSH) and catalase (CAT) levels were evaluated by reagent kits. Results. Amifostine increased SOD2 activity and expression, decreased cell injury and apoptosis, reduced mitochondrial superoxide production and intracellular ROS generation, and restored intracellular GSH and CAT levels in PC12 cells exposed to glutamate. SOD2-siRNA, however, significantly reversed the amifostine-induced cytoprotective and antioxidative actions. Conclusion. SOD2 mediates amifostine-induced protection in PC12 cells exposed to glutamate.

show abstract