Protective effects of alpinae oxyphyllae fructus (Alpinia oxyphylla MIQ) water‐extracts on neurons from ischemic damage and neuronal cell toxicity

Abstract

Alpinae Oxyphyllae Fructus (Alpinia oxyphylla MIQ) (Korean name Ik-Jj-In) is a medicinal plant used in Korea for the treatment of various symptoms accompanying hypertension and cerebrovascular disorders. The present study was performed to investigate the effects of Alpinae Oxyphyllae Fructus water-extracts (AOF) on a cultured primary neuron cell system, cell cytotoxicity and lipid peroxidation in Abeta treatment conditions. Cell killing was significantly enhanced by addition of increasing concentrations of Abeta. Pretreatment of AOF attenuated in cell killing enhanced by increasing concentrations of Abeta. Abeta-induced cell death was protected by the application of water extract of AOF in a dose-dependent manner, and concentrations of 50 to 100 micro g/ml had a significant effect compared to exposure of Abeta only. AOF has been shown to protect primary cultured neurons from N-methyl-D-aspartate (NMDA) receptor-mediated glutamate toxicity. The evidence indicated that AOF protects neurons against ischemia-induced cell death. Oral administration of AOF into mice prevented ischemia-induced learning disability and rescued hippocampal CA1 neurons from lethal ischemic damage. The neuroprotective action of exogenous AOF was also confirmed by counting synapses in the hippocampal CA1 region. The presence of AOF in neuron cultures did not repress a NMDA receptor-mediated increase in intracellular Ca(2+), but rescued the neurons from NO-induced death. AOF may exert its neuroprotective effect by reducing the NO-mediated formation of free radicals or antagonizing their toxicity.

Alpinate Oxyphyllae Fructus (Alpinia Oxyphylla Miq) Extracts Inhibit Angiotensin-II Induced Cardiac Apoptosis in H9c2 Cardiomyoblast Cells

“…25) AOF is one of the most important traditional Chinese medicines, and is reported to be a neuro-protective agent. [29][30][31][32][33] It is used to treat patients with various symptoms accompanying hypertension and cerebrovascular disorders in Korea. 30) Few studies have investigated whether AOF can prevent cardiac apoptosis in hypertension.…”

“…[29][30][31][32][33] It is used to treat patients with various symptoms accompanying hypertension and cerebrovascular disorders in Korea. 30) Few studies have investigated whether AOF can prevent cardiac apoptosis in hypertension. In this study found that AOF protective effects against Ang II-induced DNA fragmentation and apoptosis in H9c2 cells.…”

“…3313; Fax: +886-4-2207-0465; E-mail: cyhuang@mail.cmu.edu.tw might be a neuro-protective agent in both water and ethanol extracts. [29][30][31][32][33] In Korea, AOF is a medicinal plant used in the treatment of various symptoms accompanying hypertension and cerebro-vascular disorders. 30) It has been reported that AOF methanol extract has cardiotonic effectiveness.…”

“…[29][30][31][32][33] In Korea, AOF is a medicinal plant used in the treatment of various symptoms accompanying hypertension and cerebro-vascular disorders. 30) It has been reported that AOF methanol extract has cardiotonic effectiveness. 34) AOF is whether has protective and remedial effects on Ang II-stimulated H9c2 cardiomyocytes is unknown.…”

Anti-inflammatory and Anti-oxidative Effects of Alpiniae Oxyphyllae Fructus Hot Aqueous Extract in Lipopolysaccharide (LPS)-stimulated Macrophages

“…Previous studies have reported musclerelaxing 4) , anti-allergic 5) , nephroprotective 6) , neuronal cell protective [7][8][9][10] , bone loss inhibitory 11) , anti-cancer 12,13) , and anti-aging 14) effects of AOF.…”