Protective effects of a novel pyrazolecarboxamide derivative against lead nitrate induced oxidative stress and DNA damage in Clarias gariepinus

Soliman, Hatem; Hamed, Mohamed; Lee, Jae‐Seong; Sayed, Alaa El‐Din H.

doi:10.1016/j.envpol.2019.01.074

Cited by 28 publications

(16 citation statements)

References 39 publications

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“…Environmental release and accumulation of pharmaceutical products are a global concern in view of increased awareness of ecotoxicological effects (Soliman et al, 2019; FIGURE 2 | Hematoxylin-eosin-stained blood smears from catfish showing I: the normal erythrocytes (A), the deformed ones after exposure to hydroxychloroquine for 15 days (B,C), the deformed ones after exposure to hydroxychloroquine + 10 mg/l Spirulina for 15 days (D), and the deformed ones after exposure to hydroxychloroquine + 20 mg/l Spirulina for 15 days (E). Tr, tear-drop cell; Cr, crenated cell; Ac, acanthocyte; Eco, eccentric nucleus; Hec, hemolyzed cells; Mn, micronucleus; Bin, bionuclei; Lon, lobed nucleus; Shc, schistocytes; Sk, sickle cell (magnification: 1,000×).…”

Section: Discussionmentioning

confidence: 99%

Spirulina platensis Alleviated the Hemotoxicity, Oxidative Damage and Histopathological Alterations of Hydroxychloroquine in Catfish (Clarias gariepinus)

2021

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The current study aims at evaluating the toxicity of hydroxychloroquine (HCQ) as a pharmaceutical residue in catfish (Clarias gariepinus) and the protective role of Spirulina platensis (SP). Four groups were used in this study: (1) a control group, (2) a group exposed to 3.16 mg/l of HCQ, (3) a group exposed to 3.16 mg/l of HCQ + 10 mg/l of SP, and (4) a group exposed to 3.16 mg/l of HCQ + 20 mg/l of SP for 15 days of exposure. The HCQ-treated group showed a significant decline in the hematological indices and glucose, total protein, and antioxidant levels in relation to the control group, whereas the HCQ-treated group showed a significant increase in the levels of creatinine, uric acid, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) as well as the percentage of poikilocytosis and nuclear abnormalities of RBCs in relation to the control group. The histopathological evaluation of the liver indicated dilation of the central vein, vacuolization, degeneration of hepatocytes and pyknotic nuclei, as well as reduction of glomeruli, dilation of Bowman’s space, and degeneration of renal tubules in the kidney of the HCQ-treated group. Spirulina platensis (SP) rendered the hematological and biochemical indexes as well as antioxidant levels and the histological architecture to normal status in a dose-dependent manner. Accordingly, the current study recommends the use of SP to remedy the toxic effects of HCQ.

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Section: Discussionmentioning

confidence: 99%

Spirulina platensis Alleviated the Hemotoxicity, Oxidative Damage and Histopathological Alterations of Hydroxychloroquine in Catfish (Clarias gariepinus)

2021

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“…Antioxidants are considered to be good biomarkers for environmental stress and pollution studies. In this connection, catalase, superoxide dismutase, and glutathione-S-transferase are the most common antioxidants used (Sayed and Soliman 2017;Soliman et al 2019). Catalase, a ubiquitous enzyme, is the first line of defense against free radicals.…”

Section: Discussionmentioning

confidence: 99%

Bisphenol-A induced antioxidants imbalance and cytokines alteration leading to immune suppression during larval development of Labeo rohita

Faheem

Adeel

Khaliq

et al. 2020

Environ Sci Pollut Res

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Recently, the oxidative stress and immunotoxicity biomarkers have been extensively used in embryotoxicity using fish embryos as promising models especially after exposure to chemical-like environmental estrogens. Bisphenol-A (BPA) is an estrogenic endocrine disruptor and is ubiquitous in the aquatic environment. Larvae of Labeo rohita were exposed to low concentrations of BPA (10, 100, 1000 μg/l) for 21 days. Innate immune system, antioxidants parameters, and developmental alterations were used as biomarkers. Exposure to BPA caused developmental abnormalities including un-inflated swim bladder, delayed yolk sac absorption, spinal curvature, and edema of pericardium. Lipid peroxidation increased and activity of catalase (p < 0.05), superoxide dismutase (p < 0.05), and glutathione peroxidase (p < 0.01) decreased after exposure to BPA. Level of reduced glutathione also decreased (p < 0.05) in BPA-exposed group. Lower expression of tumor necrosis factor-α (p < 0.05) and interferon-γ (p < 0.001) was observed in BPA-exposed groups while expression of interleukin-10 increased (p < 0.05) in larvae exposed to 10 μg/l BPA. Moreover, exposure of BPA caused a concentration-dependent increase in expression of heat shock protein 70 (p < 0.05). The present study showed that the exposure to BPA in early life stages of Labeo rohita caused oxidative stress and suppress NF-κB signaling pathway leading to immunosuppression. The results presented here demonstrate the cross talk between heat shock protein 70 and cytokines expression.

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“…It is known that exposure of fish to different pollutants in the water ecosystem can prompt the surfeit of reactive oxygen species (ROS) which causes harmful effects to macromolecules of cells (Sureda et al 2006) because microplastics can be digested by fish and causes some effects (de Sá et al 2018;Jovanovic 2017). The fish have an intricate antioxidant system which comprises superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glutathione peroxidase (GPx), which encounter the oxidative damage of reactive oxygen species (ROS) (Sayed and Soliman 2018;Soliman et al 2019). Oxidative stress index (OSI) is a comparative indicator illustrating the interaction among the freeradical inducing agents and their antioxidants system (Fazio et al 2015;Sayed and Abul Khalil 2016).…”

Section: Introductionmentioning

confidence: 99%

Antioxidants and molecular damage in Nile Tilapia (Oreochromis niloticus) after exposure to microplastics

Hamed

Soliman

Osman

et al. 2020

Environ Sci Pollut Res

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Recently, research on the biological effects of microplastics (MPs) has grown exponentially. However, effects of MPs on freshwater fishes and the mechanisms of the biological effects of MPs were limited. So, the purpose of the current study was to clarify the effects of microplastics on oxidative stress response, DNA fragmentation, and proteinogram of the early juvenile stage of Nile Tilapia (Oreochromis niloticus). The fishes were assigned into four groups: one control, three MPs-exposed groups as 1 mg/L of MPs, 10 mg/L of MPs, and 100 mg/L of MPs respectively for 15 days and 15 days of recovery. The activities of superoxide dismutase, catalase, total peroxides, and oxidative stress index (OSI), as well as lipid peroxidation and DNA fragmentation, increased in groups exposed to MPs compared to the control group in a dose-dependent manner. In contrast, the activity of total antioxidant capacity decreased in groups exposed to MPs compared to the control group in a dose-dependent manner. The electrophoretic pattern of muscle proteins revealed alteration in the proteinogram in the MPs-exposed groups compared to control. After the recovery period, the activities of superoxide dismutase, catalase, total peroxides, total antioxidant capacity, lipid peroxidation, DNA fragmentation, and the electrophoretic pattern of muscle proteins returned to normal levels in 1 mg/L of MPs-exposed group. Combined with our previous work, these results suggest that MPs cause the overproduction of reactive oxygen species (ROS) and alters the antioxidants parameters, resulting in oxidative stress and DNA damage. The present study fosters a better understanding of the toxic effects of MPs on Tilapia as a freshwater model.

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Protective effects of a novel pyrazolecarboxamide derivative against lead nitrate induced oxidative stress and DNA damage in Clarias gariepinus

Cited by 28 publications

References 39 publications

Spirulina platensis Alleviated the Hemotoxicity, Oxidative Damage and Histopathological Alterations of Hydroxychloroquine in Catfish (Clarias gariepinus)

Spirulina platensis Alleviated the Hemotoxicity, Oxidative Damage and Histopathological Alterations of Hydroxychloroquine in Catfish (Clarias gariepinus)

Bisphenol-A induced antioxidants imbalance and cytokines alteration leading to immune suppression during larval development of Labeo rohita

Antioxidants and molecular damage in Nile Tilapia (Oreochromis niloticus) after exposure to microplastics

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