Protective effect of wild raspberry ( Rubus hirsutus Thunb. ) extract against acrylamide-induced oxidative damage is potentiated after simulated gastrointestinal digestion

Chen, Wei; Su, Hongming; Xu, Yang; Bao, Tao; Zheng, Xiaodong

doi:10.1016/j.foodchem.2015.10.024

Cited by 113 publications

(55 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar decrease in cell viability after exposure to H 2 O 2 was observed in HEK‐293, HepG2 and SW872 cells . Additionally, the damage to Caco‐2 cells by other factors, such as ethyl carbamate, ethanol and acrylamide, was mainly due to excess ROS generation . Natural antioxidants could effectively scavenge the excess ROS and subsequently protect the cells from oxidative stress.…”

Section: Discussionsupporting

confidence: 58%

Reduced nitrogen supply enhances the cellular antioxidant potential of phenolic extracts through alteration of the phenolic composition in lettuce (Lactuca sativa L.)

Zhou

Liang

Zhang³

et al. 2019

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND Nitrogen availability is an important environmental factor that determines the production of phenolic compounds in vegetables, but the relationship between low nitrogen‐induced alterations of phenolic compounds in vegetable crops and the cellular antioxidant activities of these compounds remains unclear. This study investigated the effect of reduced nitrogen supply (0.05 mmol L−1 nitrate) on phenolic metabolism in lettuce and the protective role of phenolic extracts against H2O2‐induced oxidative stress in Caco‐2 cells by determining cell damage, reactive oxygen species (ROS) content and antioxidant enzyme activities. RESULTS Reduced nitrogen supply significantly improved the accumulation of phenolic compounds in lettuce, which was partially correlated with the upregulation of genes related to the phenolic synthesis pathway. Phenolic extracts from lettuce cultivated in low‐nitrogen medium exhibited a better protective effect against H2O2‐induced oxidative damage in Caco‐2 cells than those from lettuce cultivated with adequate nitrogen. These extracts act by increasing the activities of antioxidant enzymes and, subsequently, by inhibiting ROS overproduction, which leads to a decrease in mitochondrial membrane and DNA damage. The results of HPLC and correlation analyses implied that the improvement in the protective capacity of lettuce extracts after low‐nitrogen treatment may be related, not only to the increased content of phenolic compounds, but also to the increased percentage contribution of chlorogenic acid and quercetin derivatives to the total phenolic content. CONCLUSION Reduction in nitrogen supply can be a powerful strategy to modify phenolic metabolism and composition in lettuce and, consequently, to improve their antioxidant capacity. © 2019 Society of Chemical Industry

show abstract

Section: Discussionsupporting

confidence: 58%

Reduced nitrogen supply enhances the cellular antioxidant potential of phenolic extracts through alteration of the phenolic composition in lettuce (Lactuca sativa L.)

Zhou

Liang

Zhang³

et al. 2019

J Sci Food Agric

View full text Add to dashboard Cite

show abstract

“…The daily intake of AA higher than 10 μg kg −1 bw day −1 can cause toxic effects on humans (Altunay et al ., ). Our previous studies provided the evidence that the toxicity of AA was partially based on oxidative damage (Zhang et al ., , ; Wang et al ., ), thus dietary antioxidants might have a protective role against AA‐induced toxicity (Rodríguez‐Ramiro et al ., ; Chen et al ., ; Khalifa et al ., ). Numerous studies confirmed that phytochemicals acting as antioxidants can inhibit the toxicity of AA, such as rutin (Motamedshariaty et al ., ), curcumin (Kurien, ), blueberry (Zhao et al ., 2015a) and strawberry (Zhao et al ., 2015b).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity of blueberry anthocyanin extracts and their protective effects against acrylamide‐induced toxicity in HepG2 cells

Liu

LingZhu

et al. 2017

Int J of Food Sci Tech

View full text Add to dashboard Cite

Summary In this study, the antioxidant activities of blueberry anthocyanin extracts from ten blueberry varieties were evaluated based on the methods of scavenging activities for DPPH radicals, ABTS radicals, hydroxyl radicals and ferric reducing antioxidant power (FRAP) assay. Among the ten blueberry varieties, Polaris had the highest antioxidant abilities and the largest amounts of anthocyanins identified by HPLC‐MS. The protective effects of anthocyanin extracts from Polaris (AEP) against acrylamide (AA)‐induced toxicity in HepG2 cell models were also evaluated due to the neurotoxic, genotoxic and potentially carcinogenic effects of AA. The protective effects of AEP on the damage of HepG2 cells were explored from the aspects of cell viability, T‐SOD and CAT activity and MDA level. The AEP (5, 10, 20 μg mL−1) could significantly increase cell viability (P < 0.01) and inhibit AA‐induced cytotoxicity. Polaris also markedly promoted the activity of SOD, CAT and inhibited MDA level. The results showed that AEP had strong antioxidant activities, presenting high protective effects against AA‐induced cell damage in HepG2 cell models.

show abstract

“…To assess the influence of simulated digestion on TBW rice flavonoids and their biological activity, the flavonoid extract was subjected to simulated in vitro digestion as described previously, with slight modification (Chen et al, 2016a). Briefly, 100 mg of TBFE was dissolved in 10 ml of distilled water.…”

Section: In Vitro Digestionmentioning

confidence: 99%

“…Most bioactive compounds undergo extreme gastrointestinal digestion before exerting their biological activity. Some studies have shown an increase in the biological activity of compounds subjected to in vitro digestion (Chen et al, 2016a;2016b).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and antidiabetic properties of tartary buckwheat rice flavonoids after in vitro digestion

Bao

Wang²,

et al. 2016

J. Zhejiang Univ. Sci. B

Self Cite

View full text Add to dashboard Cite

Abstract:Oxidative stress and diabetes have a tendency to alter protein, lipid, and DNA moieties. One of the strategic methods used to reduce diabetes-associated oxidative stress is to inhibit the carbohydrate-digesting enzymes, thereby decreasing gastrointestinal glucose production. Plant-derived natural antioxidant molecules are considered a therapeutic tool in the treatment of oxidative stress and diabetes. The objective of this study was to identify tartary buckwheat rice flavonoids and evaluate the effect of in vitro digestion on their antioxidant and antidiabetic properties. High performance liquid chromatography (HPLC) analysis indicated the presence of rutin as a major component and quercitrin as a minor component of both digested and non-digested flavonoids. Both extracts showed a significant antioxidant capacity, but digested flavonoids showed reduced activity compared to non-digested. There were some decreases of the antioxidant activities (2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picrylhydrazy (DPPH) radical, and ferric reducing antioxidant power (FRAP)) of digested tartary buckwheat rice flavonoids compared with non-digested. Flavonoids from both groups significantly inhibited reactive oxygen species (ROS) production and α-glucosidase activity. Both digested and non-digested flavonoids markedly increased glucose consumption and glycogen content in HepG2 cells. Tartary buckwheat rice flavonoids showed appreciable antioxidant and antidiabetic properties, even after digestion. Tartary buckwheat rice appears to be a promising functional food with potent antioxidant and antidiabetic properties.

show abstract

Protective effect of wild raspberry ( Rubus hirsutus Thunb. ) extract against acrylamide-induced oxidative damage is potentiated after simulated gastrointestinal digestion

Cited by 113 publications

References 40 publications

Reduced nitrogen supply enhances the cellular antioxidant potential of phenolic extracts through alteration of the phenolic composition in lettuce (Lactuca sativa L.)

Reduced nitrogen supply enhances the cellular antioxidant potential of phenolic extracts through alteration of the phenolic composition in lettuce (Lactuca sativa L.)

Antioxidant activity of blueberry anthocyanin extracts and their protective effects against acrylamide‐induced toxicity in HepG2 cells

Antioxidant and antidiabetic properties of tartary buckwheat rice flavonoids after in vitro digestion

Contact Info

Product

Resources

About