Antioxidant activity of blueberry anthocyanin extracts and their protective effects against acrylamide‐induced toxicity in HepG2 cells

Abstract

Summary In this study, the antioxidant activities of blueberry anthocyanin extracts from ten blueberry varieties were evaluated based on the methods of scavenging activities for DPPH radicals, ABTS radicals, hydroxyl radicals and ferric reducing antioxidant power (FRAP) assay. Among the ten blueberry varieties, Polaris had the highest antioxidant abilities and the largest amounts of anthocyanins identified by HPLC‐MS. The protective effects of anthocyanin extracts from Polaris (AEP) against acrylamide (AA)‐induced toxicity in HepG2 cell models were also evaluated due to the neurotoxic, genotoxic and potentially carcinogenic effects of AA. The protective effects of AEP on the damage of HepG2 cells were explored from the aspects of cell viability, T‐SOD and CAT activity and MDA level. The AEP (5, 10, 20 μg mL−1) could significantly increase cell viability (P < 0.01) and inhibit AA‐induced cytotoxicity. Polaris also markedly promoted the activity of SOD, CAT and inhibited MDA level. The results showed that AEP had strong antioxidant activities, presenting high protective effects against AA‐induced cell damage in HepG2 cell models.

Effect of storage on total phenolics, antioxidant capacity, and physicochemical properties of blueberry (Vaccinium corymbosum L.) jam

“…Several research groups have suggested the importance of fruit phenolics as dietary antioxidants. Compared to other fruits, berries including blueberries contain a high antioxidant capacity, generally attributed to their high concentration of phenolics such as anthocyanins (Li et al, ; Moyer, Hummer, Finn, Frei, & Wrolstad, ; Skrede, Wrolstad, & Durst, ). Olivas‐Aguirre et al () recently reported a significantly higher concentration of extractable phenols in blueberry when compared to grapes, pomegranate, raspberry or mulberry.…”

“…Indeed, previous studies carried out using high‐fat fed rats suggested a potential anti‐inflammatory effect of blueberry supplementation associated with improved glucose tolerance (Seeram et al, ). In addition, Li, Liu, Lv, Yan, and Yuan () recently evaluated the protective effects of blueberry extracts against acrylamide‐induced toxicity in HepG2 cell models and observed how Polaris , which showed the highest antioxidant potential, could significantly increase cell viability and inhibit acrylamide‐induced toxicity at anthocyanin concentrations ranging from 5 to 20 µg/ml.…”

Antioxidant Blueberry Anthocyanins Induce Vasodilation via PI3K/Akt Signaling Pathway in High-Glucose-Induced Human Umbilical Vein Endothelial Cells

“…Anthocyanins are nature's most efficacious antioxidants, and blueberries contain the highest anthocyanin contents among the tested fruits and vegetables [36]. A number of epidemiological and pharmacological studies confirm that blueberry anthocyanins possess a wonderful antioxidant capacity in vitro and in vivo [37,38]. Our previous studies have confirmed that malvidin-3-glucoside and malvidin-3-galactoside are major efficient anthocyanins in blueberries and display pronounced antioxidant properties in endothelial cells [39,40].…”

The Ability of Blackberry Juice Compared to Anthocyanin and Gallic Acid to Reduce the Harmful Effects of Acrylamide in Rats' Kidneys

“…This could be attributed to the increased the levels of GSH, SOD and TAC by polyphenols. Li et al, (2018) concluded that anthocyanin can inhibit the decrease in SOD activity as induced by ACR (44) . He suggested that anthocyanin can restore the antioxidant enzyme activity and possibly reduce the generation of free radicals.…”