Protective effect of whey proteins against nonalcoholic fatty liver in rats

Abstract

BackgroundNonalcoholic fatty liver disease (NAFLD) is the hepatic manifestation of the metabolic syndrome and can vary from hepatic steatosis to end-stage liver disease. It is the most common liver disease and its prevalence is increasing worldwide. In the present study, the effect of whey proteins on some parameters of NAFLD was investigated.ResultsOral administration of the studied whey proteins products reduced the final body weight of rats. There was a significant reduction effect (P < 0.05) of the tested proteins on hepatic triglycerides, liver enzymes (ALT and AST), lipid peroxidation (malondialdehyde level) and serum glucose. Feeding on whey proteins caused an increase in the reduced glutathione. Hepatic content of reduced glutathione was not affected by any of the used whey proteins, but it showed an increasing tendency (P > 0.05). Liver histology showed an improvement of fatty infiltration in hepatocytes from whey protein groups and gives the histology of liver a normal appearance.ConclusionsThe obtained results indicate a possible role for oral administration of whey proteins in the regulation of liver biochemistries in a rat's model of NAFLD. This regulatory effect of whey proteins was accompanied by an improvement in fatty infiltration in hepatocytes and a reduction of oxidative stress parameters.

Ameliorative Effects of Pomegranate Peel Extract on Hepatotoxicity Induced by Carbon Tetrachloride in Mice

“…The same authors added that, the antioxidant activity of both linolenic acid and phenolics are mainly due to their redox characteristics, which allow them to do as reducing agents, metal chelators, hydrogen donors and singlet oxygen quenchers. Previous studies have demonstrated that Pomegranate Peel has anti-inflammatory, antioxidant, and anti-fibrotic activity [36,37].…”

Antioxidative features of whey and its application in liver protection

“…U studiji efekta proteina surutke na nealkoholnu bolest jetre pacova [21], uočeno je da kod grupe pacova koji su tretirani alfa-laktalbuminom (0.15g/dan u trajanju od 28 dana) zabeležen najveći pad holesterola u odnosu na ostale grupe (izolat proteina surutke (WPIwhey proteine isolate), hidrolizat proteina surutke (WPHwhey proteine hydrolysate), beta-laktoglobulin (beta LGbeta-lactoglobulin) glikomakropeptid (GMP-glycomacropeptide) [21]. Svi proteini surutke testirani u ovoj studiji doveli su do značajnog pada sadržaja triglicerida u jetri, a najveći pad izazvao je hidrolizat proteina surutke WPH [21].…”

“…Svi proteini surutke testirani u ovoj studiji doveli su do značajnog pada sadržaja triglicerida u jetri, a najveći pad izazvao je hidrolizat proteina surutke WPH [21]. Pored triglicerida, oralno uzimanje proteina surutke dovelo je do pada alanin i aspartat aminotansferaze.…”

“…Pored triglicerida, oralno uzimanje proteina surutke dovelo je do pada alanin i aspartat aminotansferaze. Najniža vrednost ALT-a zabeležena je kod primene alfa laktalbumina, dok je najniža vrednost AST-a zabeležena kod primene izolata proteina surutke WPI [21]. Hranjenje pacova proteinima surutke dovelo je do smanjenja nivoa malondialdehida (MDA-malondialdehyde) (P<0.05), značajnog pokazatelja lipidne peroksidacije.…”

“…Hranjenje pacova proteinima surutke dovelo je do smanjenja nivoa malondialdehida (MDA-malondialdehyde) (P<0.05), značajnog pokazatelja lipidne peroksidacije. Oralna administracija WPI, WPH i beta LG u dozi od 0.15g/dan u trajanju od 28 dana dovela je do znača-jnog (P<0.05) povećanja nivoa glutationa (GSH), koji je neenzimski protektor od oksidativnog stresa [21]. U studijama koje su sprovedene na ljudima uočeno je da kod pacijenata sa masnom jetrom, nakon 12 nedelja korišćenja kolostruma koji je bogat proteinima surutke, dovodii do značajnog smanjenja nivoa AST-a i ALT-a [22].…”