Protective effect of topical formulations containing quercetin against UVB-induced oxidative stress in hairless mice

Akhtar

et al. 2013

Braz. J. Pharm. Sci.

The objective of this study was to develop a pharmaceutical O/W emulsion containing plant-derived polyphenol extracts and evaluate its stability and antioxidant activity. O/W emulsions were prepared using ionic surfactant polysorbate 80 (Tween 80 ® ). The odorwas adjusted with few drops of blue sea fragrance. DPPH (1,1-diphenyl-2-picrylhydrazyl) assay was used to evaluate the antioxidant activity of the plant extracts alone and emulsions containing these extracts. Physical stability was assessed by submitting the emulsions to storage at 8 °C, 25 °C, 40 °C and 40 °C + 70% RH (relative humidity) for two months. Various physical characteristics of emulsions monitored, include color, creaming, liquefaction, centrifugation and pH. Brookfield rotational rheometer was used to determined viscosities and rheological behavior of emulsions. Different types of emulsion were determined microscopically, while pH values of emulsions were measured by a pH meter. Electrical conductivity data confirmed that the outer phase was water. Samples presented an acceptable pH value for an external topical use. Shear thinning behaviour was observed for all emulsions. The polyphenol-rich-plant-derived extracts alone and the extract containing emulsions showed good antioxidant activities. This research confirmed that the method used was suitable for preparing emulsions with Hippophae rhamnoids and Cassia fistula extracts, suggesting that those emulsions are suitable for topical use. O presente estudo objetivou o desenvolvimento de uma emulsão farmacêutica óleo-água contendo extratos de plantas ricos em polifenóis, a comparação à sua formulação-controle e a avaliação de sua estabilidade, assim como de sua capacidade antioxidante. Extrato concentrado de Hippophae rhamnoids e Cassia fistula foi encapsulado no interior da fase oleosa da emulsão O/W. As emulsões foram preparadas usando o tensoativo iônico monooleato sorbital de polioxietileno (Tween 80 ® ). O odor foi ajustado pela adição de algumas gotas de fragrância azul do mar. O ensaio do DPPH (1,1-difenil-2-picrilidrazil) foi utilizado para avaliar a atividade antioxidante dos extratos de plantas sozinhos e nas emulsões contendo os extratos. A estabilidade física foi avaliada submetendo os cremes a diferentes temperaturas de estocagem, como a 8 °C, 25 °C e 40 °C e a 40% + 70% de umidade relativa por um período de 2 meses. As características físicas das emulsões foram monitoradas por 2 meses incluindo cor, cremosidade, liquefação, centrifugação e pH. O reômetro rotacional de Brookfield foi utilizado para determinar a viscosidade e o comportamento reológico das emulsões. O programa Rheocalc Brookfield foi utilizado para análise dos dados. As características organolépticas também foram avaliadas. O tipo de emulsão foi determinado microscopicamente, enquanto o pH das emulsões foi avaliado por meio de um pHmetro. A estabilidade farmacêutica esperada das emulsões foi alcançada dentro dos dois meses de estudo. Os resultados da condutividade elétrica confirmaram que a fase externa da emulsão era c...

Section: Discussionmentioning

confidence: 99%

Development, characterization and antioxidant activity of polysorbate based O/W emulsion containing polyphenols derived from Hippophae rhamnoides and Cassia fistula

Akhtar

et al. 2013

Braz. J. Pharm. Sci.

“…15 Quercetin is a promising compound to combat free radical diseases such as cancer and inflammatory conditions. Furthermore, its topical effectiveness to protect the skin from oxidative stress has been recently demonstrated, 6,13,16 which reinforces the importance of quality control of topical formulation containing quercetin. In this sense, this work demonstrates one of the most valuable tools in the pharmaceutical analysis of quality control, 11 which is a HPLC assay for the analysis of quercetin in topical formulations.…”

Section: Resultsmentioning

confidence: 81%

“…Its use in topical emulsion reduces UVB-induced oxidative and inflammatory skin damages. 6 High performance liquid chromatography (HPLC) is a useful tool to analyze samples of complex nature like ointments and creams, because it provides not only the separation and determination, but also eliminates most interference problems. 7 The first demonstration of the applicability of HPLC to determine flavonoids was in 1976, 8 and since then, several studies have described the validation of HPLC methods for quantifying flavonoids.…”

Section: Introductionmentioning

confidence: 99%

Method validation and stability study of quercetin in topical emulsions

Casagrande¹,

Baracat²,

Georgetti³

et al. 2009

Quím. Nova

Self Cite

. do Café, s/n, 14040-903 Ribeirão Preto -SP, BrasilRecebido em 7/8/08; aceito em 11/3/09; publicado na web em 4/8/09This study validated a high performance liquid chromatography (HPLC) method for the quantitative evaluation of quercetin in topical emulsions. The method was linear within 0.05 -200 µg/mL range with a correlation coefficient of 0.9997, and without interference in the quercetin peak. The detection and quantitation limits were 18 and 29 ng/mL, respectively. The intra-and inter-assay precisions presented R.S.D. values lower than 2%. An average of 93% and 94% of quercetin was recovered for non-ionic and anionic emulsions, respectively. The raw material and anionic emulsion, but not non-ionic emulsion, were stable in all storage conditions for one year. The method reported is a fast and reliable HPLC technique useful for quercetin determination in topical emulsions.

“…Rosmarinik asit hücreler arası lipit peroksidasyonunu azaltmakta ve UVA'nın reaktif oksijen türlerini artırıcı etkilerini önemli oranda baskılamaktadır 72 . Sophora japonica bitkisinde yüksek antioksidan özellikte olan kersetin bulunmaktadır ve ciltte meydana gelebilecek UVB kaynaklı oksitatif hasarı ortadan kaldırmaya yardımcı olmaktadır 73 . 44 .…”

Section: Bitki Ekstreleri Ve Bileşenleriunclassified

Anti-Aging Cosmetics Approaches and Components in Products

Yapar¹,

Tanrıverdi²

2016

BSBD

Cilt yaşlanması; iç veya dış faktörlerin veya bunların birlikteliğinin neden olduğu karmaşık biyolojik bir süreçtir. Gelişen üretim teknolojileri, farmasötik taşıyıcı sistemlerin kozmetik formüllerinde kullanılması ve yenilikçi bileşenlere dayalı yeni nesil kozmetik ürünler, temel kozmetik bakımdan ileri alternatifler sunmaktadır. İleri teknoloji kullanılarak üretilen kozmetik aktif bileşenleri, doğal kaynaklı hammaddelerin kozmetik bileşenleri olarak kullanımının artması ve farmasötik taşıyıcı sistemlerin kozmetik formüllerinde kullanılması ile geliştirilen kozmetik ürünler toplumların artan kozmetik beklentilerini karşılamayı hedeflemektedir. Bu derlemede, günümüzde bireylerin dış ve iç faktörler ile artan cilt yaşlanmasının, kozmetik ürün odaklı çözümleri özellikle bileşenler açısından irdelenmiş, yaşlanma karşıtı farklı yaklaşımlar özetlenmiş ve kullanılan taşıyıcı sistemler belirtilmiştir.