Method validation and stability study of quercetin in topical emulsions

Casagrande, Rúbia; Baracat, Marcela M.; Georgetti, Sandra R.; Verri, Waldiceu A.; Vicentini, Fabiana T. M. C.; Rafael, Janice Aparecida; Jabor, José Roberto; Fonseca, Maria José Vieira

doi:10.1590/s0100-40422009000700041

Cited by 9 publications

(9 citation statements)

References 10 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During the study, both emulsions remained physically stable and pH values remained satisfactory, which ensures that F1 and F2 are compatible with the application site, avoiding irritation (24). Regarding functional stability study of DPPH scavenging activity, it was observed that temperature, storage time, and type of formulation influenced the AA of PPE.…”

Section: In Vivo Studiesmentioning

confidence: 82%

“…With the aim of evaluating the physico-chemical characteristics, the following tests were performed: visual evaluation (color, consistence, and phase separation) (24); pH measurement in triplicate (10% dilution in deionized water); evaluation of phase separation in triplicate (2 g of formulation submitted to centrifugation at 145×g for 30 min) (13,25).…”

Section: Evaluation Of Physico-chemical Characteristics Of Formulationsmentioning

confidence: 99%

See 1 more Smart Citation

Topical Formulations Containing Pimenta pseudocaryophyllus Extract: In Vitro Antioxidant Activity and In Vivo Efficacy Against UV-B-Induced Oxidative Stress

et al. 2013

Self Cite

View full text Add to dashboard Cite

Abstract. Pimenta pseudocaryophyllus is a Brazilian native plant that presents high concentrations of flavonoids and other polyphenolic compounds. Herein, we evaluated: (1) the chemical properties of P. pseudocaryophyllus ethanolic extract (PPE), (2) the in vitro antioxidant activity (AA) of PPE and of two different topical formulations (F1 and F2) containing PPE, (3) physico-chemical and functional stability, (4) in vitro release of PPE, and (5) in vivo capacity of formulations to prevent UV-B irradiation-induced skin damage. Results show that the polyphenol and flavonoid contents in PPE were 199.33 and 28.32 mg/g, respectively, and HPLC results show the presence of eugenol, tannic acid, and rutin. Evaluation of the in vitro AA of PPE demonstrated a dose-dependent effect and an IC 50 of 4.75 μg/mL in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 3.0 μg/mL in 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. The ferric-reducing antioxidant power (FRAP assay) was 0.046 μmol/L trolox equivalent/ μg/mL of extract. Among the AA, only the capacity to scavenge DPPH radical of PPE was maintained in F1 and F2. In addition, both formulations satisfactorily released the extract. The evaluation of the functional stability of F1 and F2 did not demonstrate loss of activity by storage at room temperature and at 4°C/6 months. In irradiated mice, treatment with F1 and F2 added with PPE significantly increased the capacity to scavenge ABTS radical and the FRAP of skin compared to vehicle-treated mice. In conclusion, the present results suggest that formulations containing PPE may be a topical source of antioxidant compounds to decrease oxidative damages of the skin.

show abstract

Section: In Vivo Studiesmentioning

confidence: 82%

Section: Evaluation Of Physico-chemical Characteristics Of Formulationsmentioning

confidence: 99%

Topical Formulations Containing Pimenta pseudocaryophyllus Extract: In Vitro Antioxidant Activity and In Vivo Efficacy Against UV-B-Induced Oxidative Stress

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…In order to analyze complex samples, such as extract, high performance liquid chromatography (HPLC) is a good tool not only for separating target compounds, but also for revealing the peaks interfering with the analytes. 23 In the 60 years since the first analytical method for quantifying boldine in boldo was published, 24 several other methods using various approaches including densitometry, 25 spectrofluorimetry, 26,27 micellar electrokinetic chromatography, 28,29 voltammetry, 30,31 and LC-NMR have been described. 32 The high performance liquid chromatography (HPLC) method coupled to a UV detector was developed by Pietta et al 33 and this procedure is currently used in different pharmacopoeias for pharmaceutical boldo preparations.…”

Section: Introductionmentioning

confidence: 99%

Study of Quality Assurance For Peumus Boldus M Products By Botanic Profiling, Extraction Optimization, HPLC Quantification And Antioxidant Assay

Teixeira¹,

Cabral²,

Sousa³

et al. 2016

Phcogj

View full text Add to dashboard Cite

Introduction:The boldo leaf has several traditional folk medicinal uses, such as for gallbladder, hepatic problems, digestive disorders, rheuma tism and others. In the work reported herein, botanic profiling, extraction optimization by Soxhlet, quantification of boldine by an easy/simple to run HPLC method and antioxidant assay are proposed for the quality assurance of boldo dried leaves, standardized extracts, dried extracts, tablets and capsules. Materials and Methods: In this present work we are studying a systematic approach in the quality assurance study of quality assurance for Peumus boldus M products by the ascertainment of pharmacobotanic parameters for boldo identification, investigation of the extraction para meters by Soxhlet method, development and validation of an easy/simple to run HPLC method to quantify boldine in the raw drug, extracts, commercial tablets, capsules and coated tablets, and antioxidant assay. Results: The plant material was submitted to a pharmacognostic evaluation through morphoanatomical diagnosis, showing that starlike trichomes can be used for boldo authentication. The HPLC validated analytical method is reliable, accurate and precise for boldine quantification. Furthermore, the Soxhlet extraction conditions were optimized. Conclusion: The methods proposed in this paper can be used for the quality assurance of boldo dried leaves,

show abstract

“…19,20 In order to analyze complex samples, such as ointments and creams, high performance liquid chromatography (HPLC) is a good tool not only for separating target compounds, but also for revealing the peaks interfering with the analytes. 21 Moreover, to develop a phytotherapic, it is important to validate an analytical method which presents good performance parameters such as selectivity, linearity, accuracy, precision and recovery over the specified range that an analyte is analyzed. 22 Therefore, the aim of the present work was to develop and validate an HPLC method to quantify SN and SM in a topical formulation and their respective skin penetration.…”

Section: Introductionmentioning

confidence: 99%

A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies

Tiossi¹,

Costa²,

Miranda³

et al. 2012

Quím. Nova

View full text Add to dashboard Cite

Recebido em 30/5/12; aceito em 25/8/12; publicado na web em 9/11/12To assess topical delivery studies of glycoalkaloids, an analytical method by HPLC-UV was developed and validated for the determination of solasonine (SN) and solamargine (SM) in different skin layers, as well as in a topical formulation. The method was linear within the ranges 0.86 to 990.00 µg/mL for SN and 1.74 to 1000.00 µg/mL for SM (r = 0.9996). Moreover, the recoveries for both glycoalkaloids were higher than 88.94 and 93.23% from skin samples and topical formulation, respectively. The method developed is reliable and suitable for topical delivery skin studies and for determining the content of SN and SM in topical formulations.

show abstract

Method validation and stability study of quercetin in topical emulsions

Cited by 9 publications

References 10 publications

Topical Formulations Containing Pimenta pseudocaryophyllus Extract: In Vitro Antioxidant Activity and In Vivo Efficacy Against UV-B-Induced Oxidative Stress

Topical Formulations Containing Pimenta pseudocaryophyllus Extract: In Vitro Antioxidant Activity and In Vivo Efficacy Against UV-B-Induced Oxidative Stress

Study of Quality Assurance For Peumus Boldus M Products By Botanic Profiling, Extraction Optimization, HPLC Quantification And Antioxidant Assay

A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies

Contact Info

Product

Resources

About