Protective effect of Toki-shakuyaku-san on amyloidβ25-35-induced neuronal damage in cultured rat cortical neurons

Abstract

Amyloid beta protein (Abeta) is the major component of senile plaques, the pathological hallmark of the neurodegeneration associated with Alzheimer's disease (AD). This study investigated the effect of Toki-shakuyaku-san (TSS), a traditional medicine, on Abeta25-35-induced neuronal death and lipid peroxidation assessed by measuring lactate dehydrogenase (LDH) and malondialdehyde (MDA), respectively. Abeta25-35 at 10 microM induced neuronal damage and increased the LDH and MDA. TSS at concentrations of 100 and 300 microg/mL significantly reduced the Abeta25-35-induced neuronal death and the lipid peroxidation. These results suggest that TSS has a protective effect against Abeta25-35-induced neuronal damage. TSS may be beneficial for the treatment of AD.

Danggui-Shaoyao-San: New Hope for Alzheimer's Disease

“…DSS inhibited Aβ25-35-induced neuronal damage and lactate dehydrogenase (LDH). The Aβ25-35-induced neuronal death and lipid peroxidation were significantly reduced by DSS at concentrations of 100 and 300 µg/mL [63]. Through DSS administration, the SCOP-induced impairment including reference and working memory deficits of a rat's spatial cognition determined in the eightarmed radial maze test was decreased [65].…”

Danggui-Shaoyao-San ameliorates cognition deficits and attenuates oxidative stress-related neuronal apoptosis in d-galactose-induced senescent mice

“…For more than thousand years, DSS has been used to improve post-menopausal disorders with neurological symptoms, and regulate hypothalamic-pituitary-endocrine system and immune-neuroendocrine functions in China (Kou et al, 2005;Goto et al, 2009). Recently, many studies have showed that DSS had therapeutic efficacy for senile dementia by enhancing the cognitive behavior (Kou et al, 2002;Hatip-AI-Khatib et al, 2004;Hu et al, 2010), improving the mitochondrial impairment (Kou et al, 2005) and inhibiting neuronal damage (Egashira et al, 2005;Qian et al, 2008;Hwang et al, 2011). These abovementioned findings provide some pharmacological basis for the dementia prevention function of DSS.…”

Dangguijakyak-san, a medicinal herbal formula, protects dopaminergic neurons from 6-hydroxydopamine-induced neurotoxicity

“…In animal studies, DJS improves spatial cognition impairment (Egashira et al, 2004), central cholinergic nervous system dysfunction (Itoh et al, 1996), and A␤-induced spatial recognition deficits (Hu et al, 2010). DJS also has neuroprotective effects in hydrogen peroxide-induced toxicity in PC12 cells (Qian et al, 2008), A␤ 25-35 -induced neuronal damage in cultured rat cortical neurons, and senile dementia in aged mice (Egashira et al, 2005).…”