Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen

Bucak, Mustafa Numan; Tekin, Necmettin

doi:10.1016/j.smallrumres.2006.12.001

Cited by 128 publications

(81 citation statements)

References 31 publications

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“…Semen samples were collected from six WAD goat bucks with the aid of an artificial vagina and pooled. A total of six semen samples (each semen sample originating from six bucks) showing >80% motility was pooled to minimize individual differences (Bucak and Tekin, 2007). Semen samples were diluted at 32°C in a two-step process (Watson, 1995;Purdy, 2006) with a Tris-egg yolk based extender composed of 2 fractions.…”

Section: Semen Collection Dilution and Storagementioning

confidence: 99%

Protective effects of fruit-juices on sperm viability of West African Dwarf goat bucks during cryopreservation

et al. 2016

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The present study was conducted to determine the effects of adding fruit-juices to semen extenders on viability of buck spermatozoa during cryopreservation in two studies. Pooled semen samples obtained from West African Dwarf (WAD) goat bucks were diluted with Tris-egg yolk based extenders. In study I, the diluted semen samples were supplemented with orange (Citrus sinensis), cucumber (Cucumis sativus) and pineapple (Ananas comosus) juices (2.5, 5, 7.5 and 10 ml/100 ml). In study II, the diluted semen samples were supplemented with 5 ml/100 ml of different combinations of fruitjuices consisting of pineapple and orange juices (TEYPO) at a 1:1 ratio, pineapple and cucumber juices (TEYPC) at a 1:1 ratio, orange and cucumber juices (TEYOC) at a 1:1 ratio, and pineapple, orange and cucumber juices (TEYPOC) at a 1:1:1 ratio, respectively. The diluted semen samples were cryopreserved and thereafter evaluated for sperm viability parameters. The extenders supplemented with 7.5 and 10% orange, and 10% pineapple had higher (P < 0.05) motility compared to the control. There was higher (P < 0.05) acrosome integrity in extenders supplemented with orange and pineapple at all levels compared to the control while cucumber at 10% had higher (P < 0.05) acrosome integrity compared to other levels and the control. The extenders supplemented with cucumber, orange and pineapple had higher (P < 0.05) membrane integrity compared to the control except at 7.5% of extender supplemented with cucumber. Similarly, the extenders supplemented with cucumber, orange and pineapple had lower (P < 0.05) sperm abnormality compared to the control. The extenders supplemented with cucumber, orange and pineapple at 2.5, 10 and 7.5% respectively had lower (P < 0.05) concentrations of MDA compared to other levels and the control. There was higher motility and acrosome integrity in extenders supplemented with different combinations of fruit-juices (P < 0.05) compared to the control. TEYPC had higher (P < 0.05) membrane integrity and lower (P < 0.05) concentrations of MDA compared to the control. The findings revealed that extenders supplemented with orange and pineapple at 10% consistently improved motility, acrosome integrity and membrane integrity, and reduced sperm abnormality compared to the control while no particular combination of these fruit-juices was consistently superior in all the parameters.

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Section: Semen Collection Dilution and Storagementioning

confidence: 99%

Protective effects of fruit-juices on sperm viability of West African Dwarf goat bucks during cryopreservation

et al. 2016

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“…Therefore, it is a key product of lipid peroxidation in the cells (3,35). GSH is a multifunctional non-protein which is known as a thiol group antioxidant in mammalian cells (12,33,36). This compound counterattack the effects of oxidative stress in sperm cells directly and indirectly (throughout cooperation with GSH-PX for reduction of toxic H2O2) (12,33,36).…”

Section: Discussionmentioning

confidence: 99%

“…Their depredatory effects can be specified as irreversible reduction on motility and viability of spermatozoa, and moreover, permanent damages on plasma membrane, acrosome and DNA (11,12). On natural conditions, antioxidant system elements of spermatozoa and seminal plasma protect the spermatozoa against the lipid peroxidation (3).…”

Section: Introductionmentioning

confidence: 99%

Positive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5C

ALÇAY

2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The aim of the study was to evaluate different BSA doses in soybean-lecithin based extenders for liquid storage of ram semen. Pooled semen was divided into four equal volumes and diluted in a single-step dilution method with BSA supplemented (BSA5, BSA7.5, BSA10 groups supplemented 5mg/mL, 7.5mg/mL, 10mg/mL BSA respectively) and BSA-free (control) extenders. Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypo-osmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) at 0, 24, 48 and 72h of storage at 5 °C. Antioxidant activities of groups at 0 and 72h were measured with glutathionine (GSH) concentration, membrane lipid peroxidation status was analyzed using the malondialdehyde (MDA) concentration. Antioxidant capacity of extender also was measured with 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assays. In the study, BSA (5mg/mL) supplementation has beneficial effect on ram sperm parameters at all evaluation times. In addition, the increasing doses of BSA supplementation has a negative effect on sperm parameters especially 48 and 72h of storage.Keywords: BSA, lecithin, ram semen, short-term storage.BSA ilave edilmiş lesitin bazlı sulandırıcının koç spermasının 5 C sıcaklıkta muhafaza edilmesine olumlu etkisi Özet: Bu çalışmada, farklı dozlarda BSA ilave edilmiş lesitin bazlı sulandırıcının koç spermasının 5°C'ta kısa sureli saklanması üzerindeki etkisinin değerlendirilmesi amaçlandı. Çalışma amacıyla alınan spermalar pooling yapılarak dört eşit hacme bölündü ve 5mg/mL, 7.5mg/mL, 10mg/mL BSA ilave edilmiş (BSA5, BSA7.5, BSA10 sırasıyla) ve edilmemiş (kontrol) sulandırıcılarla sulandırıldı. Sulandırılmış spermanın sıcaklığı 5°C'a düştükten sonra 0, 24, 48 ve 72. saatlerde; motilite, plazma membran fonksiyonel bütünük [hipoozmotik şişme testi (HOST)] ve akrozomal hasar [FITC-Pisum sativum agglutinin (PSA-FITC)] muayeneleri yapıldı. Çalışmanın başlangıcı ve sonunda antioksidan aktivitenin ve lipid peroksidasyonun değerlendirilmesi için malondialdehyde (MDA) ve glutathionine (GSH) konsantrasyonları ölçüldü. Ayrıca 2,2-Diphenyl-1-picrylhydrazyl (DPPH) ve Dichloro-dihydro-fluorescein diacetate (DCFH-DA) yöntemleri ile her bir sulandırıcının antioksidan kapasiteleri değerlendirildi. Yapılan muayenelerin sonucunda; 5mg/mL BSA ilavesinin koç spermasının kısa süreli muhafazasında etkili olduğu gözlemlendi. Ayrıca artan BSA konsantrasyonunun spermanın soğutularak saklanmasında olumsuz etki oluşturduğu belirlendi. Anahtar sözcükler: BSA, kısa süreli muhafaza, koç sperması, lesitin.

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“…Several investigations were carried out over the past years to reduce the negative effects of ROS accumulation and lipid peroxidation on spermatozoa exposed to cryopreservation and thawing stress by supplementing the extender with different antioxidants (Donoghue & Donoghue, 1997;Bucak and Tekin, 2007;Câmara et al, 2011;Ashrafi et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Improving cryopreservation capacity of ram spermatozoa by supplementing the diluent with melatonin

Khalifa¹

2017

IJAR

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Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen

Cited by 128 publications

References 31 publications

Protective effects of fruit-juices on sperm viability of West African Dwarf goat bucks during cryopreservation

Protective effects of fruit-juices on sperm viability of West African Dwarf goat bucks during cryopreservation

Positive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5C

Improving cryopreservation capacity of ram spermatozoa by supplementing the diluent with melatonin

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