Protective effects of fruit-juices on sperm viability of West African Dwarf goat bucks during cryopreservation

Abstract

The present study was conducted to determine the effects of adding fruit-juices to semen extenders on viability of buck spermatozoa during cryopreservation in two studies. Pooled semen samples obtained from West African Dwarf (WAD) goat bucks were diluted with Tris-egg yolk based extenders. In study I, the diluted semen samples were supplemented with orange (Citrus sinensis), cucumber (Cucumis sativus) and pineapple (Ananas comosus) juices (2.5, 5, 7.5 and 10 ml/100 ml). In study II, the diluted semen samples were supplemented with 5 ml/100 ml of different combinations of fruitjuices consisting of pineapple and orange juices (TEYPO) at a 1:1 ratio, pineapple and cucumber juices (TEYPC) at a 1:1 ratio, orange and cucumber juices (TEYOC) at a 1:1 ratio, and pineapple, orange and cucumber juices (TEYPOC) at a 1:1:1 ratio, respectively. The diluted semen samples were cryopreserved and thereafter evaluated for sperm viability parameters. The extenders supplemented with 7.5 and 10% orange, and 10% pineapple had higher (P < 0.05) motility compared to the control. There was higher (P < 0.05) acrosome integrity in extenders supplemented with orange and pineapple at all levels compared to the control while cucumber at 10% had higher (P < 0.05) acrosome integrity compared to other levels and the control. The extenders supplemented with cucumber, orange and pineapple had higher (P < 0.05) membrane integrity compared to the control except at 7.5% of extender supplemented with cucumber. Similarly, the extenders supplemented with cucumber, orange and pineapple had lower (P < 0.05) sperm abnormality compared to the control. The extenders supplemented with cucumber, orange and pineapple at 2.5, 10 and 7.5% respectively had lower (P < 0.05) concentrations of MDA compared to other levels and the control. There was higher motility and acrosome integrity in extenders supplemented with different combinations of fruit-juices (P < 0.05) compared to the control. TEYPC had higher (P < 0.05) membrane integrity and lower (P < 0.05) concentrations of MDA compared to the control. The findings revealed that extenders supplemented with orange and pineapple at 10% consistently improved motility, acrosome integrity and membrane integrity, and reduced sperm abnormality compared to the control while no particular combination of these fruit-juices was consistently superior in all the parameters.

Addition of orange, pineapple and beet juices as extenders for cryopreservation of ram semen

“…The study carried out by Fukuhara and Nishikawa (1973) (28) showed a significant effect of increasing energy to maintain motility in goat sperm when a variety of sugars was added, but there was no significant difference caused by sucrose, unlike glucose and fructose, which promoted improvements. Daramola et al (2016) (16) , studying cucumber, pineapple and orange juices added to cryopreserved ram semen diluent, found higher values of progressive motility, especially when using concentrations of 7.5 and 10%. In the present study, pineapple and orange juices did not produce similar results, unlike beet juice, which was statistically better regarding progressive motility than the others.…”

“…The juices were prepared according to the procedures described by Adeyemo et al (2007) (15) and Daramola et al (2016) (16) from pineapple (Ananas comosus L. var. Pearl), sweet orange (Citrus sinensis L.) and beet (Beta vulgaris L).…”

Adição de sucos de laranja, abacaxi e beterraba em diluidor para criopreservação de sêmen de carneiros

“…O mesmo tratamento também manteve o percentual de motilidade total acima de 30%, em linha com as características desejáveis para carneiros quanto à dose de sêmen congelado segundo o CBRA (2013) (21) . Os açúcares podem promover melhorias no equilíbrio osmótico e na crioproteção das células espermáticas, além de serem utilizados na ocorrência de fosforilação oxidativa (9,16) . Wruss et al (2015) (27) , ao analisarem o suco de sete diferentes variedades de beterraba, encontraram valores entre 60% de betacianinas e 40% de betaxantinas, que representaram de 70 a 100% do total de compostos fenólicos.…”

“…Daramola et al (2016) (16) , estudando sucos de pepino, abacaxi e laranja adicionados ao diluente de sêmen ovino criopreservado, encontraram maiores valores de motilidade progressiva, principalmente quando se utilizaram concentrações de 7,5 e 10%. No presente estudo, os sucos de abacaxi e laranja não obtiveram resultados semelhantes, diferentemente do suco de beterraba, que foi estatisticamente melhor em motilidade progressiva que os demais.…”

“…Os sucos de frutas e beterraba foram preparados de acordo com os procedimentos descritos por Adeyemo et al (2007) (15) e Daramola et al (2016) (16) utilizando abacaxi (Ananas comosus L. var. Pearl), laranja doce (Citrus sinensis L.) e beterraba (Beta vulgaris L).…”