Protective Effect of Tanshinone IIA on the Early Stage of Experimental Diabetic Nephropathy

Kim, Su Kang; Jung, Kyung‐Hwan; Lee, Byung-Cheol

doi:10.1248/bpb.32.220

Cited by 42 publications

(29 citation statements)

References 32 publications

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“…The progression of CRF is characterized histologically by progressive glomerulosclerosis and tubulointerstitial fibrosis (9,36). A significant observation of the present study was the marked improvement effect of SKG on renal pathological damage, through inhibition of the relative area of renal glomerulosclerosis and tubulointerstitial fibrosis, particularly in the SKGL and SKGM groups.…”

Section: Discussionsupporting

confidence: 50%

Effect of Shenkang granules on the progression of chronic renal failure in 5/6 nephrectomized rats

Zhang

Zhou

Wang

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. Shenkang granules (SKGs) are a Chinese herbal medicinal formula, consisting of rhubarb (Rheum palmatum L.), Salvia miltiorrhiza, milkvetch root [Astragalus membranaceus (Fisch.) Bunge] and safflower (Carthamus tinctorius L.). The aim of the present study was to investigate the effect of SKG on chronic renal failure (CRF) in 5/6 nephrectomized (5/6 Nx) rats. The rats were randomly divided into seven groups (n=10 per group) as follows: (i) 5/6 Nx (model group; 2.25 ml/kg/day normal saline); (ii) SKGL (low dose; 5/6 Nx treated with 2 g crude drug/kg/day SKG); (iii) SKGM (moderate dose; 5/6 Nx treated with 4 g crude drug/kg/day SKG); (iv) SKGH (high dose; 5/6 Nx treated with 8 g crude drug/kg/day SKG); (v) benazepril treatment group (5/6 Nx treated with 5 mg/kg/day benazepril); (vi) Shenkang injection (SKI) group (5/6 Nx with 13.3 ml/kg/day SKI); and (vii) sham-operated group (2.25 ml/kg/day normal saline). After 30 days, the levels of microalbumin, total protein, serum creatinine, blood urea nitrogen and serum lipid were found to be significantly decreased in the SKGL and SKGM rats, showing histological improvement compared with the untreated 5/6 Nx rats, as determined by hematoxylin and eosin, and Masson's trichrome staining. In addition, SKG was found to significantly improve the levels of glutathione peroxidase and reduce the damage caused by free radicals to the kidney tissues. Furthermore, SKG prevented the accumulation of extracellular matrix by decreasing the expression of collagen I and III and inhibiting the expression of matrix metalloproteinases-2 and -9 in the renal tissue, as determined by western blot analysis. SKG was also shown to decrease the concentrations of serum transforming growth factor-β 1 , as determined by ELISA, and kidney angiotensin II, as determined using a radioimmunoassay kit. In conclusion, SKG was demonstrated to ameliorate renal injury in a 5/6 Nx rat model of CRF. Thus, SKG may exert a good therapeutic effect on CRF.

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Section: Discussionsupporting

confidence: 50%

Effect of Shenkang granules on the progression of chronic renal failure in 5/6 nephrectomized rats

Zhang

Zhou

Wang

et al. 2015

Experimental and Therapeutic Medicine

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“…Pioglitazone attenuates DN by downregulating TGF-β and NFκB in type II diabetic rats (43). Kim et al (13) identified that TGF-β was decreased in DN rat models treated with TⅡA. In the present study, the results revealed that TGFβ and p65 mRNA and protein expression were upregulated in DN rats and downregulated following treatment with TⅡA.…”

Section: Discussionsupporting

confidence: 62%

“…As a multi-functional agent in TCM, TⅡA has a fundamental role in cellular responses to extracellular stimuli. For example, TⅡA protects cardiomyocytes in part through B-cell lymphoma 2/B-cell lymphoma 2-associated X protein and Akt-signaling pathways (14)(15), is able to produce an inhibitory effect on the sterol regulatory element-binding transcription factor 1 pathway through the phosphoinositide 3-kinase/Akt signaling pathway (16) and significantly inhibits the expressions of numerous factors, including transforming growth factor β (TGFβ), in a rat model of DN (13). However, only the primary action of TⅡA on DN has been studied, and the mechanisms underlying how TⅡA ameliorates DN require further elucidation.…”

Section: Introductionmentioning

confidence: 99%

Role of the TGFβ/p65 pathway in tanshinone IIA-treated HBZY-1 cells

Chen

Zhang

et al. 2014

Molecular Medicine Reports

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Abstract. Tanshinone ⅡA (TⅡA) is widely used for the treatment of a number human diseases, including diabetic nephropathy (DN) (1). The present study was performed to examine the role of the transforming growth factor β (TGFβ)/p65 pathway under TⅡA treatment in a glomerular mesangial cell model of DN. Firstly, it was identified that TⅡA inhibited the proliferation of HBZY-1 cells, while simultaneously suppressing the expression of TGFβ and p65. In addition, glucose-induced HBZY-1 cells were treated with TⅡA, si-TGFβ and si-p65. The results revealed that si-TGFβ or si-p65 were able to inhibit the proliferation of HBZY-1 cells as well. Finally, the expression of TGFβ and p65 in a rat model of DN treated with TⅡA was detected. The results demonstrated that renal hypertrophy and 24 h urinary protein excretion were ameliorated in TⅡA-treated rats with DN. Furthermore, it was revealed that the protein levels of TGFβ and p65 were decreased in the DN rats following TⅡA treatment. In conclusion, the present study demonstrated that TGFβ and p65 were activated by TⅡA in HBZY-1 cells. In addition, the expression of TGFβ and of p65 was downregulated in rats with DN treated with TⅡA.

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“…Salvianolic acid B was found to have anti-inflammatory, anti-fibrogenic, skip flap ischemia-reperfusion attenuation, atherosclerosis reduction, and cyclooxygenase-2 attenuation properties [6][7][8][9][10], in addition to being an efficient radical scavenger and antioxidant [11]. Likewise, tanshinone IIA is also characterized by anti-inflammation activity, anti-oxidation, and cardio-protection, as well as neuro-protection, anticancer and diabetic nephropathy prevention [12][13][14][15][16][17]. Remarkably, both salvianolic acid B and tanshinone IIA were also found to inhibit atherogenesis hyperlipid activity [18].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of salvianolic acid B and tanshinone IIA of salvia tropolone tablets by UPLC-MRM-MS/MS for pharmacokinetic studies

Li¹,

Li²,

Li³

et al. 2014

Acta Chromatographica

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Summary.A rapid and sensitive ultraperformance liquid chromatography-multiple reaction monitoring-multi-stage/mass spectrometry (UPLC-MRM-MS/MS) method has been developed for simultaneous quantification of salvianolic acid B and tanshinone IIA of salvia tropolone tablets in dog plasma. This was achieved by performing quantification using the MRM acquisition with two channels of MRM-MS/MS and MS full scan for more accuracy qualitative results, and the fragmentation transitions of m/z 295→249, 191 for tanshinone IIA and m/z 297→279, 251 for IS in positive mode, m/z 717→519, 321 for salvianolic acid B and m/z 295→267, 239 for IS in negative mode were selected. The UPLC separation was achieved within 3 min in a single UPLC run. Linear calibration curves were obtained over the concentration range of 10 pg/mL −1 ng/mL for tanshinone IIA and 100 pg/mL −1 for salvianolic acid B. Lower limit of quantitation (LLOQ) was 10 pg/mL and 100 pg/mL for tanshinone IIA and salvianolic acid B, respectively. The inter-day and intra-day precision (relative standard deviation, RSD) in all samples were less than 8.21%, and the recoveries were over 85.9% for both tanshinone IIA and salvianolic acid B. The two channels of MRM with MS full scan approach could provide both qualitative and quantitative results without the need for repetitive analyses and resulted in the reduction of further confirmation experiments and analytical time. The pharmacokinetic study of the two active components of salvia tropolone tablets following oral gavage administration of dogs was thus explored with this method.

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Protective Effect of Tanshinone IIA on the Early Stage of Experimental Diabetic Nephropathy

Cited by 42 publications

References 32 publications

Effect of Shenkang granules on the progression of chronic renal failure in 5/6 nephrectomized rats

Effect of Shenkang granules on the progression of chronic renal failure in 5/6 nephrectomized rats

Role of the TGFβ/p65 pathway in tanshinone IIA-treated HBZY-1 cells

Simultaneous quantification of salvianolic acid B and tanshinone IIA of salvia tropolone tablets by UPLC-MRM-MS/MS for pharmacokinetic studies

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