Simultaneous quantification of salvianolic acid B and tanshinone IIA of salvia tropolone tablets by UPLC-MRM-MS/MS for pharmacokinetic studies

Li, J. R.; Li, D. X.; Li, L.; Deng, Wen; Ding, Lianhui; Hong, Xu; Zhou, Yang

doi:10.1556/achrom.26.2014.4.12

Cited by 3 publications

(1 citation statement)

References 29 publications

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“…Similar to the detection of many drug molecules at nanogram level, it is difficult to quantitatively detect LSB in plasma by HPLC coupled with a UV–Visible detector due to its low bioavailability and high binding affinity to plasma proteins [ 20 ]. In a previous study, LSB of 300–90,000 pg/mL in dog plasma was effectively detected via removing the plasma interference by using LC/MS spectrometry in MRM mode [ 26 ]. In this study, LSB at nanogram level in rat plasma was successfully monitored via avoiding plasma interference through the selection of parent and corresponding product ions of LSB (m/z 717 → 519, 321) under the same technical approach.…”

Section: Discussionmentioning

confidence: 99%

Detection of lithospermate B in rat plasma at the nanogram level by LC/MS in multi reaction monitoring mode

Chung

Lin

et al. 2018

Journal of Food and Drug Analysis

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Low bioavailability and high binding affinity to plasma proteins led to the difficulty for the quantitative detection of lithospermate B (LSB) in plasma. This study aimed to develop a protocol for detecting LSB in plasma. A method was employed to quantitatively detect LSB of 5-500 ng/mL by LC/MS spectrometry in multi reaction monitoring mode via monitoring two major fragments with m/z values of 519 and 321 in the MS2 spectrum. To set up an adequate extraction solution to release LSB captured by plasma proteins, recovery yields of LSB extracted from rat plasma acidified by formic acid or HCl in the presence or absence of EDTA and caffeic acid were detected and compared using the above quantitative method. High recovery yield (∼90%) was achieved when LSB (5-500 ng/mL) mixed in rat plasma was acidified by HCl (5 M) in the presence of EDTA (0.5 M) and caffeic acid (400 μg/mL). The lower limit of detection and the lower limit of quantification for LSB in the spiked plasma were calculated to be 1.8 and 5.4 ng/mL, respectively. Good accuracy (within ±10%) and precision (less than 10%) of intra- and inter-day quality controlled samples were observed. Oral bioavailability of LSB in rat model was detected via this optimized extraction method, and the maximum plasma concentration (C) was found to be 1034.3 ± 510.5 μg/L at t around 10 min, and the area under the plasma concentration-time curve (AUC) was 1414.1 ± 851.2 μg·h/L.

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Section: Discussionmentioning

confidence: 99%

Detection of lithospermate B in rat plasma at the nanogram level by LC/MS in multi reaction monitoring mode

Chung

Lin

et al. 2018

Journal of Food and Drug Analysis

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Pharmacokinetics of 13 active components in a rat model of middle cerebral artery occlusion after intravenous injection of Radix Salviae miltiorrhizae‐Lignum dalbergiae odoriferae prescription

Shi

Feng

et al. 2019

J of Separation Science

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As a representative formulation of Radix Salviae miltiorrhizae (Danshen)‐Lignum Dalbergiae odoriferae (Jiangxiang), Xiangdan injection is widely prescribed for cardio‐ and cerebrovascular diseases in practice. This necessitates a pharmacokinetic investigation of this formulation to make it safer and more broadly applicable. We developed and validated a sensitive, selective, and reliable high‐performance liquid chromatography with tandem mass spectrometry method for the simultaneous determination of 11 phenolic compounds including danshensu plus two diterpenoid quinones like cryptotanshinone and tanshinone IIA in rat. We applied this method for the pharmacokinetic studies of the 13 compounds in a rat model of middle cerebral artery occlusion after intravenous injection of Xiangdan injection or Danshen injection. In sham‐operated rats, the animals taking Xiangdan injection exhibited significant growth of the area under the curve for danshensu, protocatechuic aldehyde, and tanshinone IIA compared with the changes seen in the data of those administrated with Danshen injection. Such a pattern was also observed in middle cerebral artery occlusion rats, whereas increased the area under the curve values were observed for danshensu, protocatechuic aldehyde, caffeic acid, rosmarinic acid, and tanshinone IIA. These results demonstrated that synergistic interactions occurred between the components of Danshen and the active compounds of Jiangxiang both in sham‐operated and middle cerebral artery occlusion rats, increasing the bioavailability of Danshen. The results presented herein can be used to determine a reference dose for the clinical application of Xiangdan injection, and to elucidate the synergistic mechanism of Danshen and Jiangxiang.

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Identification of Chemical Constituents in Blumea balsamifera Using UPLC–Q–Orbitrap HRMS and Evaluation of Their Antioxidant Activities

et al. 2023

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Blumea balsamifera (L.) DC., a perennial herb in the Asteraceae family native to China and Southeast Asia, has a notable history of medicinal use due to its pharmacological properties. Using UPLC–Q–Orbitrap HRMS techniques, we systematically investigated the chemical constituents of this plant. A total of 31 constituents were identified, of which 14 were flavonoid compounds. Significantly, 18 of these compounds were identified in B. balsamifera for the first time. Furthermore, the mass spectrometry fragmentation patterns of significant chemical constituents identified in B. balsamifera were analyzed, providing important insights into their structural characteristics. The in vitro antioxidative potential of the methanol extract of B. balsamifera was assessed using DPPH and ABTS free-radical-scavenging assays, total antioxidative capacity, and reducing power. The antioxidative activity exhibited a direct correlation with the mass concentration of the extract, with IC50 values of 105.1 ± 0.503 μg/mL and 12.49 ± 0.341 μg/mL for DPPH and ABTS, respectively. For total antioxidant capacity, the absorbance was 0.454 ± 0.009 at 400 μg/mL. In addition, the reducing power was 1.099 ± 0.03 at 2000 μg/mL. This study affirms that UPLC–Q–Orbitrap HRMS can effectively discern the chemical constituents in B. balsamifera, primarily its flavonoid compounds, and substantiates its antioxidative properties. This underscores its potential utility as a natural antioxidant in the food, pharmaceutical, and cosmetics sectors. This research provides a valuable theoretical basis and reference value for the comprehensive development and utilization of B. balsamifera and expands our understanding of this medicinally valuable plant.

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Simultaneous quantification of salvianolic acid B and tanshinone IIA of salvia tropolone tablets by UPLC-MRM-MS/MS for pharmacokinetic studies

Cited by 3 publications

References 29 publications

Detection of lithospermate B in rat plasma at the nanogram level by LC/MS in multi reaction monitoring mode

Detection of lithospermate B in rat plasma at the nanogram level by LC/MS in multi reaction monitoring mode

Pharmacokinetics of 13 active components in a rat model of middle cerebral artery occlusion after intravenous injection of Radix Salviae miltiorrhizae‐Lignum dalbergiae odoriferae prescription

Identification of Chemical Constituents in Blumea balsamifera Using UPLC–Q–Orbitrap HRMS and Evaluation of Their Antioxidant Activities

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