Protective Effect of T. violacea Rhizome Extract Against Hypercholesterolemia-Induced Oxidative Stress in Wistar Rats

Abstract

The present study examines the effect of methanolic extract of T. violacea rhizomes on high cholesterol (2%) diet fed rats (HCD). At the end of 4 weeks, serum total protein, albumin, reduced glutathione (GSH), and markers of oxidative stress viz., catalase (CAT), superoxide dismutase (SOD), thiobarbituric acid reactive substances (TBARSa marker of lipid peroxidation), glutathione-S-transferase (GST) and glutathione peroxidase (GPx) in the serum, aorta, liver and heart of HCD and normal rats were assessed and compared. A significant (p < 0.05) elevation in TBARS, and a reduction (p < 0.05) in serum total protein, albumin, GSH and antioxidant enzyme activities was observed in tissues of HCD fed rats compared with the normal group. Co-administration of crude extracts of T. violacea rhizomes protected the liver, heart, serum and aorta against HCD-induced lipid peroxidation in a dose dependant manner. The activities of the extract (500 mg/kg) compared favorably with gemfibrozil. The extracts also protected against HCD-induced reduction in serum total protein, GSH and restored the activities of antioxidant tissues (liver, heart and aorta) enzymes to near normal values. This result suggested that consumption of T. violacea rhizome may help to protect against hypercholesterolemia-induced oxidative stress diseases in the heart and liver.

Amelioration of oxidative and inflammatory status in hearts of cholesterol-fed rats supplemented with oils or oil-products with extra virgin olive oil components

“…The above hypothesis is further supported by Molinar-Toribio et al [46] who showed that glutathione system was not activated in the hearts of SHROB rats. However, a decrease in GSH has been observed in the hearts of female cholesterol-fed Wistar rats [47].…”

Potential involvement of PPAR α activation in diminishing the hepatoprotective effect of fenofibrate in NAFLD

“…Gemfibrozil (GF) (Sigma Aldrich chemicals, Germany) was supplied as white powder, suspended in small amount of tween 80 and distilled water and was given with daily oral gavage at a dose of 100 mg/kg [47].…”

Identification of modulated mRNAs and proteins in human primary hepatocytes treated with non-steroidal anti-inflammatory drugs

“…The trafficking depends on the activities of hepatic LPL that hydrolyses lipoproteins 13 . in the present study, the significant downregulation of LPL in the sulindac-diclofenac group might indicate elevation of lipoprotein in the blood and 14 have recently reported that hypercholesterolemia increases the level of lipid peroxidation in the liver. Therefore, it could be expected that both sulindac and diclofenac might lead to lethal liver cell injury by lipid peroxidation via down-regulating LPL and hyperlipidemia.…”