Protective effect of sucupira oil nanoemulsion against oxidative stress in UVA-irradiated HaCaT cells

Abstract

Objectives Bioactive molecules derived from natural products combine the ability to absorb UV light and act as antioxidants. We developed an oil‐based sucupira (native species of the Brazilian cerrado) nanoemulsion (NE) using a high‐energy emulsification method and assessed its effectiveness in vitro. Methods An easily scalable high‐pressure homogenization method was used to prepare the formulation. NE droplets mean diameter, pH, stability, conductivity and morphology were analysed. Formulation bioactivity was assessed using HaCaT cells. Key findings The formulation presented suitable pH and size for topic administration and was stable for over 90 days upon storage at 4, 25 and 45°C. The NE showed protective effect against oxidative stress and reduced levels of UVA‐induced pro‐inflammatory cytokines IL‐6 and IL‐8. Conclusions A novel, stable and easily prepared formulation was obtained for encapsulation of sucupira oil. The protective effect of the formulation by cytokine inhibition in the early stage of the inflammatory process was shown in vitro. Combined with the antioxidant effect by inhibition of reactive oxygen species, the use of sucupira oil NE for prevention and treatment of UVA‐induced stress could contribute to decrease the effects of UV radiation on skin ageing.

Facile Fabrication of Sandalwood Oil-Based Nanoemulsion to Intensify the Fatty Acid Composition in Burned and Rough Skin

“…The study of photostable molecules derived from natural products for the development of lotions is gaining popularity. However, the synergistic effects of secondary metabolites in plants allow for the acquisition of multiple biological effects from a single product . In the present study, the role of oxidative stress in the generation of intracellular ROS was investigated in the HaCaT cell line.…”

“…However, the synergistic effects of secondary metabolites in plants allow for the acquisition of multiple biological effects from a single product. 54 In the present study, the role of oxidative stress in the generation of intracellular ROS was investigated in the HaCaT cell line. Higher ROS levels appeared in cells treated with commercial serum (0.25 and 0.50%) through elevated green fluorescence as compared to cells treated with herbal nanoserum (0.25 and 0.50%) and untreated control cells.…”

The role of bioactive phytoconstituents-loaded nanoemulsions for skin improvement: a review

“…Natural plant-based bioactive constituents are the more attractive and safer substitutes over synthetic anti-oxidants as active ingredients in topical nanoemulsions to improve skin quality. Anti-aging properties in compounds, phenolic acids, terpenoids, polyunsaturated fatty acids, lycopene, resveratrol, lignan, tannins, indoles, flavonoids, coenzyme q, polysaccharides and anthocyanins have been investigated and studied extensively [130]. The anti-oxidants mentioned above can be extracted from other parts of the plant, including fruits, grains, seeds, roots, leaves, stems and flowers.…”

Recent progress in micro and nano-encapsulation of bioactive derivatives of the Brazilian genus Pterodon

“…In parallel to the antioxidant effects, the NE managed to modulate the inflammatory profile of epithelial cells. A reduction in the levels of pro-inflammatory cytokines, IL-6 and IL-8, was observed in keratinocytes after UV irradiation [ 134 ]. Despite the high energy emulsification method and heating (at 65 °C) used to produce the nanoformulation, the oil activity was preserved.…”