Protective effect of some selected fruit blends on testicular toxicity in alloxan-induced diabetic rats

Obode, Okukwe C.; Okafor, Oluwatoyin; Erukainure, Ochuko L.; Ajayi, Atinuke; Suberu, Yewande; Ogunji, Akinyele; Okporua, Teressa; Oluwole, Oluwatoyin; Ozumba, AU; Elemo, Gloria N.

doi:10.1515/jcim-2014-0032

Cited by 9 publications

(10 citation statements)

References 20 publications

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“…We believe that the increased prostatic CAT expression in the MH group may be induced in response to intrauterine oxidative stress. Although there is a lack of studies demonstrating the effect of GDM on offspring reproductive organs, we believe that our results can be related to those of Obode et al (), who demonstrated increased CAT activity in testes of alloxan‐induced diabetic in adult rats and associated these results with increased oxidative stress and reduced quality of sperm in diabetic rats.…”

Section: Discussionsupporting

confidence: 82%

Streptozotocin‐Induced Maternal Hyperglycemia Increases the Expression of Antioxidant Enzymes and Mast Cell Number in Offspring Rat Ventral Prostate

Camargo

Santos

Rinaldi

et al. 2016

The Anatomical Record

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Gestational diabetes mellitus (GDM) has increased in recent years. Although the cellular and molecular mechanisms involved in GDM-increased risk factors to offspring remained poorly understood, some studies suggested an association between an increase in oxidative stress induced by maternal hyperglycemia and complications for both mothers and newborns. Here, we investigated the impact of maternal hyperglycemia followed by maternal insulin replacement during lactation on the expression of antioxidant enzymes and mast cell number in offspring ventral prostate (VP) at puberty. Pregnant rats were divided into three groups: control (CT); streptozotocin-induced maternal hyperglycemia (MH); and MH plus maternal insulin replacement during lactation (MHI). Male offspring were euthanized at postnatal day (PND) 60 and the VP was removed and processed for histology and Western blotting analyses. Maternal hyperglycemia delayed prostate maturation, and increased mast cell number catalase (CAT), superoxide dismutase (SOD), glutatione-s-transferase (GST-pi), and cyclooxygenase-2 (Cox-2) expression in the offspring of hyperglycemic dams. Maternal insulin replacement restored VP structure, mast cell number and antioxidant protein expression, except for Cox-2, which remained higher in the MHI group. Thus, an increase in oxidative stress induced by intrauterine hyperglycemia impacts prostate development and maturation, which persists until puberty. The overall improvement of maternal metabolism after insulin administration contributes to the restoration of prostate antioxidant enzymes and secretory function. Taken together, our results highlighted that imbalanced physiological maternal-fetal interaction contributes to the impairment of reproductive performance of the offspring from diabetic mothers. Anat Rec, 300:291-299, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 82%

Streptozotocin‐Induced Maternal Hyperglycemia Increases the Expression of Antioxidant Enzymes and Mast Cell Number in Offspring Rat Ventral Prostate

Camargo

Santos

Rinaldi

et al. 2016

The Anatomical Record

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“…Alterations of these pathways have been implicated in the onset of macro‐ and microvascular diseases (Boden, ; Kilarkaje et al, ). Of particular interest is the impairment of the male reproductive system, which is characterized by peroxidation of the testicular PUFAs constituents, morphological changes in the testes, and erectile dysfunction among others (Kilarkaje et al, ; Obode et al, ; Xu et al, ). In this study, the therapeutic effect of Raffia palm wine was investigated in hyperglycaemia‐induced oxidative injury in T2D.…”

Section: Discussionmentioning

confidence: 99%

“…Male infertility has been recognized as one of the complications of diabetes mellitus (DM) (Dias, Alves, Silva, & Oliveira, 2014;Kilarkaje, Al-Hussaini, & Al-Bader, 2014;Xu et al, 2014). This has been attributed to the polyunsaturated fatty acids (PUFAs)-rich content of the testicular tissues, which is highly susceptible to oxidative damage from hyperglycaemia-generated free radicals (Erukainure et al, 2012;Obode et al, 2015). Oxidative damage occurs when the generated free radicals overwhelms the testicular endogenous antioxidant system, leading to impaired spermatogenesis and steroidogenesis, and finally infertility (Alves et al, 2013;Xu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Raffia palm (Raphia hookeri) wine extenuates redox imbalance and modulates activities of glycolytic and cholinergic enzymes in hyperglycemia-induced testicular injury in type 2 diabetic rats

2019

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This study investigated the effects of Raffia palm wine (RPW) on redox imbalance, glycolytic and cholinergic enzymes, and ATPase activities in hyperglycemia‐induced oxidative testicular injury. Type 2 diabetes (T2D) was induced in male albino rats (Sprague‐Dawley) by first administering 10% fructose solution for 14 days, before injecting with an intraperitoneal injection (40 mg/kg body weight) of streptozotocin. Raffia palm wine was administered to two diabetic groups at 150 and 300 mg/kg body weight (bw), when untreated diabetic group was used as a negative control, and metformin‐fed group was served as a positive control. The rats were sacrificed after 5 weeks of treatment, and testes were harvested. Treatment with RPW led to lower levels of nitric oxide, malondialdehyde and myeloperoxidase activity, with concomitant elevation of reduced glutathione level, superoxide dismutase, catalase and ATPase activities. Raffia palm wine also inhibited glycogen phosphorylase, glucose‐6‐phosphatase, fructose‐1,6‐bisphosphatase, and acetylcholinesterase, while restoring the altered testicular morphology to near‐normal. The results of this study suggest the therapeutic potentials of RPW against the effects of T2D on testicular functions and morphology. Practical applications Diabetes mellitus is one of the fastest growing global epidemy, with most developing countries being at the receiving end owing to the cost of treatment. Testicular damage has been recognized as one of the complications of diabetes, and if left untreated will lead to erectile dysfunction followed by infertility. Raffia palm wine is among the common natural beverage in West Africa, which is utilized for both social and medicinal purposes. In this study, for the first time we showed its ability to protect diabetes‐induced testicular injury in type 2 diabetic rats. This may be of great benefit in managing diabetes associated erectly dysfunction and male infertility, as Raffia palm wine is readily available in all seasons. This study will also improve the medicinal use of this wine, which may also indirectly improve its commercial benefit.

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“…Therefore, many studies indicated that guavas have anti-inflammatory, anti-microbial, anti-oxidative, anticancer, and anti-diarrheal functions (25)(26)(27). Many studies have demonstrated that guavas can improve anti-oxidant functions such as glutathione levels and activities of superoxide dismutase, catalase, and glutathione peroxidase against oxidative stress-induced damage (28)(29)(30)(31). Guavas can inhibit arsenic-induced (29), streptozotocin-induced (30,31), and alloxan-induced (28) oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have demonstrated that guavas can improve anti-oxidant functions such as glutathione levels and activities of superoxide dismutase, catalase, and glutathione peroxidase against oxidative stress-induced damage (28)(29)(30)(31). Guavas can inhibit arsenic-induced (29), streptozotocin-induced (30,31), and alloxan-induced (28) oxidative stress. Furthermore, a recent study suggested that guavas can inhibit caspase activity to attenuate cell apoptosis in type II diabetic rats (32).…”

Section: Introductionmentioning

confidence: 99%

Extracts from guava fruit protect renal tubular endothelial cells against acetaminophen‑induced cytotoxicity

Liu

Lin

et al. 2018

Mol Med Report

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Acetaminophen (APAP) is an analgesic and antipyretic agent primarily used in the clinical setting. However, high doses of APAP can cause oxidative stress. Guavas have been reported to provide anti‑inflammatory, anti‑microbial, anti‑oxidative and anti‑diarrheal functions. In addition, guavas have been reported to prevent renal damage due to progression of diabetes mellitus. Therefore, the aim of the present study was to investigate whether guavas can reduce APAP‑induced renal cell damage. In the present study, extracts from guavas were obtained and added to APAP‑treated renal tubular endothelial cells. The present results demonstrated that APAP induces cytotoxicity in renal tubular endothelial cells, while guava extracts inhibited this cytotoxicity. In addition, the study demonstrated that the protective effects of guava extracts against APAP‑induced cytotoxicity may be associated with inhibition of oxidative stress and caspase‑3 activation.

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Protective effect of some selected fruit blends on testicular toxicity in alloxan-induced diabetic rats

Abstract: This study shows an improvement in testicular antioxidant activities and sperm qualities by single and double doses of the formulation, suggesting its protective potential against testicular toxicity in diabetic rats.

Cited by 9 publications

References 20 publications

Streptozotocin‐Induced Maternal Hyperglycemia Increases the Expression of Antioxidant Enzymes and Mast Cell Number in Offspring Rat Ventral Prostate

Streptozotocin‐Induced Maternal Hyperglycemia Increases the Expression of Antioxidant Enzymes and Mast Cell Number in Offspring Rat Ventral Prostate

Raffia palm (Raphia hookeri) wine extenuates redox imbalance and modulates activities of glycolytic and cholinergic enzymes in hyperglycemia-induced testicular injury in type 2 diabetic rats

Extracts from guava fruit protect renal tubular endothelial cells against acetaminophen‑induced cytotoxicity

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