Protective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague–Dawley rats

Abstract

An increased accumulation of intracellular levels of reactive oxygen species with time may play an important role in the process of ageing. The antioxidant properties of resveratrol are dependent upon the up-regulation of endogenous cellular antioxidant systems. We evaluated whether resveratrol has protective antioxidant effects on ovarian damage related to oxidative stress in a rat model. Twenty-four female rats were randomly divided into three groups and were given saline (group 1: control); intraperitoneal cisplatin, 4.5 mg/kg, two weekly doses in total (group 2); or cisplatin, 4.5 mg/kg plus intraperitoneal resveratrol 10 mg/kg/day, 24 h before the administration of cisplatin (group 3). Serum anti-Müllerian hormone (AMH) concentrations were significantly lower in group 2 than in group 3 (P < 0.01 and P = 0.04, respectively). The evaluation of the atretic and antral follicle counts revealed statistically significant differences between the groups (P = 0.04 and P < 0.01, respectively). A statistically significant difference was observed in the follicle count positive for AMH between the groups (P = 0.01). Oxidative stress plays an important role in the process of ovarian ageing. Because of its natural antioxidant properties, resveratrol may be an effective option in protecting ovarian tissue against oxidative damage.

Age-associated up-regulation of EGR1 promotes granulosa cell apoptosis during follicle atresia in mice through the NF-κB pathway

“…32 It has been established that the increase in ROS-induced damage during aging plays a vital role in ovarian aging. 33 The present study showed that EGR1 plays a role in promoting apoptosis in GCs under H 2 O 2 stimulation. We found that overexpression of EGR1 could aggravate ROS-induced damage to GCs, indicating that EGR1 up-regulation may accelerate apoptosis in GCs during ovarian aging.…”

Can Coenzyme Q10 supplementation protect the ovarian reserve against oxidative damage?

“…Although the mechanism of cisplatin toxicity on ovaries has not been clearly explained, in addition to DNA damage, it may be due to increased ROS production and decreased antioxidant capacity, which is similar to the demonstrated toxic effect of cisplatin on the kidneys and internal ear [19,21,22]. In our previous study, we demonstrated that cisplatin may cause oxidative stress-related ovarian damage, and this effect can be handled by an antioxidant [23]. Therefore, in the present study, cisplatin was used for OS occurring in ovarian tissue.…”

“…Power calculation was based on serum AMH levels [23]. The results were analysed using the Statistical Package for the Social Sciences version 21.0 (SPSS, Chicago, IL).…”

“…The immunohistochemistry procedure was performed as described in our previous study [23]. The anti-Mullerian hormone (AMH)-positive follicles were analysed according to two factors: the intensity (0 to 3 as follows: 0, no staining; 1, weak staining; 2, moderate staining; and 3, strong staining) and the distribution of staining (1 or 2, as follows: 1, ≤50 % of the structure staining and 2, ≥50 % of the structure staining) [28].…”

Resveratrol promotes in vitro activation of ovine primordial follicles by reducing DNA damage and enhancing granulosa cell proliferation via phosphatidylinositol 3‐kinase pathway

“…Resveratrol protected the rat ovary against chromium toxicity (Banu et al, ) and irradiation (Simsek et al, ) by enhancing endogenous antioxidant enzymes. Moreover, pre‐treatment of rats with resveratrol before the administration of cisplatin reduced the atretic follicles compared to the group that only received cisplatin (Özcan et al, ). Supplementation of the maturation medium with resveratrol increased blastocyst formation and decreased the expression of proapoptotic genes (caspase‐3 and/or bax) (swine: Kwak et al, ; bovine: Wang et al, ; caprine: Mukherjee et al, ).…”