Resveratrol promotes in vitro activation of ovine primordial follicles by reducing DNA damage and enhancing granulosa cell proliferation via phosphatidylinositol 3‐kinase pathway

Bezerra, M. É. S.; Gouveia, B.B.; Barberino, Ricássio de Sousa; Menezes, Vanúzia Gonçalves; Macedo, T. J. S.; Cavalcante, A.Y.P.; Monte, Alane Pains Oliveira do; Santos, J.M.S.; Matos, Maria Helena Tavares de

doi:10.1111/rda.13274

Cited by 24 publications

(23 citation statements)

References 33 publications

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“…In cancer cells, RES has a powerful anti-inflammatory effect and antioxidative role and has been found to induce cellular apoptosis and cell-cycle arrest [40,41,42]. Bezerra et al [43] discovered that RES promotes primordial follicle activation by reducing DNA fragmentation and stimulates granulosa cell proliferation through activation of the PI3K pathway. Li et al [44] showed that RES reduces oxidative stress and inhibits apoptosis in granulosa cells by activating the PI3K/AKT signaling pathway in a rat model of POI.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol Plays a Protective Role against Premature Ovarian Failure and Prompts Female Germline Stem Cell Survival

Jiang

Zhao-yuan

Cha

et al. 2019

IJMS

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This study was designed to investigate the protective effect of resveratrol (RES) on premature ovarian failure (POF) and the proliferation of female germline stem cells (FGSCs) at the tissue and cell levels. POF mice were lavaged with RES, and POF ovaries were co-cultured with RES and/or GANT61 in vitro. FGSCs were pretreated with Busulfan and RES and/or GANT61 and co-cultured with M1 macrophages, which were pretreated with RES. The weights of mice and their ovaries, as well as their follicle number, were measured. Ovarian function, antioxidative stress, inflammation, and FGSCs survival were evaluated. RES significantly increased the weights of POF mice and their ovaries as well as the number of follicles, while it decreased the atresia rate of follicles. Higher levels of Mvh, Oct4, SOD2, GPx, and CAT were detected after treatment with RES in vivo and in vitro. RES treatment resulted in significantly lower TNF-α and IL-6 concentrations and an obviously higher IL-10 concentration in the ovaries. In FGSCs, higher Mvh, Oct4, and SOD2 concentrations and lower TNF-α, IL-6, and MDA concentrations were measured in the RES group. Blockage of the Hh signaling pathway reversed the protective effect of RES on FGSCs. In conclusion, RES effectively improved the ovarian function of the POF model and the productive capacity of FGSCs via relieving oxidative stress and inflammation and a mechanism involving the Hh signaling pathway, suggesting that RES is a potential agent against POF and can aid in the survival of FGSCs.

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Section: Discussionmentioning

confidence: 99%

Resveratrol Plays a Protective Role against Premature Ovarian Failure and Prompts Female Germline Stem Cell Survival

Jiang

Zhao-yuan

Cha

et al. 2019

IJMS

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“…However, marker expression varied between the investigated species, as well as with the maturation stage of isolated granulosa cells. For example, Oct-4 expression was detected in human but not in porcine model immediately after isolation of GCs [ 44 , 45 ]. While this fact is one of the many indicators that the stemness of granulosa cells needs to be further investigated to become a consensus, it is certainly a population of high potential for possible applications in the fields of modern medicine.…”

Section: Stemness Of Human Ovarian Granulosa Cellsmentioning

confidence: 99%

“…These findings stay in accordance with results of Bazerra et al, reporting that resveratrol enhances primordial follicle activation and decreases the level of DNA fragmentation in in vitro cultures of ovine ovarian tissue. Furthermore, they have also shown the ability of resveratrol to stimulate GCs proliferation via activation of the PI3K pathway [ 44 , 97 ]. PI3K is known as one of the fundamental signaling pathways, involved in follicle activation in rodents [ 44 ].…”

Section: The Modulation Of Granulosa Cell Physiological Processes mentioning

confidence: 99%

“…Furthermore, they have also shown the ability of resveratrol to stimulate GCs proliferation via activation of the PI3K pathway [ 44 , 97 ]. PI3K is known as one of the fundamental signaling pathways, involved in follicle activation in rodents [ 44 ]. Interestingly, in cancer cell lines, resveratrol has been reported to down-regulate the PI3K/AKT pathway, as well as induce apoptosis and exert chemotherapeutic activity [ 98 ].…”

Section: The Modulation Of Granulosa Cell Physiological Processes mentioning

confidence: 99%

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The Stemness of Human Ovarian Granulosa Cells and the Role of Resveratrol in the Differentiation of MSCs—A Review Based on Cellular and Molecular Knowledge

Józkowiak

Hutchings

Jankowski

et al. 2020

Cells

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Ovarian Granulosa Cells (GCs) are known to proliferate in the developing follicle and undergo several biochemical processes during folliculogenesis. They represent a multipotent cell population that has been differentiated to neuronal cells, chondrocytes, and osteoblasts in vitro. However, progression and maturation of GCs are accompanied by a reduction in their stemness. In the developing follicle, GCs communicate with the oocyte bidirectionally via gap junctions. Together with neighboring theca cells, they play a crucial role in steroidogenesis, particularly the production of estradiol, as well as progesterone following luteinization. Many signaling pathways are known to be important throughout the follicle development, leading either towards luteinization and release of the oocyte, or follicular atresia and apoptosis. These signaling pathways include cAMP, PI3K, SMAD, Hedgehog (HH), Hippo and Notch, which act together in a complex manner to control the maturation of GCs through regulation of key genes, from the primordial follicle to the luteal phase. Small molecules such as resveratrol, a phytoalexin found in grapes, peanuts and other dietary constituents, may be able to activate/inhibit these signaling pathways and thereby control physiological properties of GCs. This article reviews the current knowledge about granulosa stem cells, the signaling pathways driving their development and maturation, as well as biological activities of resveratrol and its properties as a pro-differentiation agent.

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“…Excessive ROS can retard oocyte maturation and lead to cell death [30, 31]. Supplementation of the environment with antioxidants could decrease oxidative damage and thus protect cells [32–34]. N-Acetyl-L-cysteine (NAC) is less toxic and can increase intracellular glutathione (GSH) levels [35, 36], and GSH plays a key role in preventing oxidative stress in oocytes [37].…”

Section: Introductionmentioning

confidence: 99%

Influence of N-acetyl-L-cysteine against bisphenol a on the maturation of mouse oocytes and embryo development: in vitro study

Zhao

2019

BMC Pharmacol Toxicol

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Background Bisphenol A (BPA), an endocrine disruptor, is a widely used chemical that has adverse effects on animal development and reproduction. The current research aimed to evaluate the effect of BPA on the in vitro maturation (IVM) and subsequent embryo development of mouse oocytes following in vitro fertilization (IVF). Methods IVM was performed in the presence of different concentrations (0, 20, 50, or 100 μg/mL) of BPA. Nuclear maturation, IVF efficiency and embryonic development were determined. The levels of reactive oxygen species (ROS) and glutathione (GSH) in the BPA (50 μg/mL) group were evaluated. We explored the ability of N-acetyl-L-cysteine (NAC) in the IVM medium to rescue the BPA-induced damage by examining changes in nuclear maturation, IVF rate, blastocyst formation, ROS levels and GSH content. Results Compared with the control, BPA (50 μg/mL) supplementation during oocyte IVM significantly inhibited nuclear maturation and decreased fertilization and blastocyst formation rates. In addition, BPA exposure increased ROS levels and decreased GSH content in oocytes. The addition of NAC weakened the BPA-induced suppression of nuclear maturation, relieved the BPA-induced downregulation of the fertilization and blastocyst formation rates, and mitigated the increased ROS levels and decreased GSH content. Conclusion BPA affects mouse oocyte maturation and subsequent early embryonic developmental competence following IVF by increasing intracytoplasmic oxidative stress in mature oocytes. NAC can reduce these harmful effects to a certain extent. Electronic supplementary material The online version of this article (10.1186/s40360-019-0323-9) contains supplementary material, which is available to authorized users.

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Resveratrol promotes in vitro activation of ovine primordial follicles by reducing DNA damage and enhancing granulosa cell proliferation via phosphatidylinositol 3‐kinase pathway

Cited by 24 publications

References 33 publications

Resveratrol Plays a Protective Role against Premature Ovarian Failure and Prompts Female Germline Stem Cell Survival

Resveratrol Plays a Protective Role against Premature Ovarian Failure and Prompts Female Germline Stem Cell Survival

The Stemness of Human Ovarian Granulosa Cells and the Role of Resveratrol in the Differentiation of MSCs—A Review Based on Cellular and Molecular Knowledge

Influence of N-acetyl-L-cysteine against bisphenol a on the maturation of mouse oocytes and embryo development: in vitro study

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