Protective Effect of Pyropia yezoensis Peptide on Dexamethasone-Induced Myotube Atrophy in C2C12 Myotubes

Lee, Min‐Kyeong; Choi, Jeong‐Heui; Choi, Youn Hee; Nam, Taek‐Jeong

doi:10.3390/md17050284

Cited by 18 publications

(11 citation statements)

References 55 publications

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“…Since the mTOR signaling pathway is mainly studied in our research and essential in protein synthesis process, GILZ may also bind to mTORC2 to inhibit phosphorylation of AKT (at Ser473) and activate FoxO3a-mediated transcription of the pro-apoptotic protein BIMIN to inhabit protein synthesis procedure under stress condition. In L6 myoblasts, GCs inhibited IGF1 secretion and the phosphorylation levels of 4EBP1 and P70S6K1, which play key roles in protein synthesis, thereby reducing skeletal muscle protein synthesis [ 34 ]. In our study, the phosphorylation levels of mTOR, P70S6K, and 4EBP1 were all significantly decreased after DEX treatment in PSCs, indicating that GCs reduce the protein synthesis levels in skeletal muscle by downregulating mTOR signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

“…GCs suppress protein synthesis to induced muscle atrophy through a variety of mechanisms, among which the catabolism of GCs inhibits the transport of amino acids into muscle cells, thereby reducing protein synthesis [ 34 , 42 ]. Cells will have an increasing demand for amino acids to satisfy the body’s needs in special events like cancer or stress which are detrimental to cell growth or development [ 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

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Glucocorticoid Regulates the Synthesis of Porcine Muscle Protein through m6A Modified Amino Acid Transporter SLC7A7

Guo

Shi

et al. 2022

IJMS

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The occurrence of stress is unavoidable in the process of livestock production, and prolonged stress will cause the decrease of livestock productivity. The stress response is mainly regulated by the hypothalamic-pituitary-adrenal axis (HPA axis), which produces a large amount of stress hormones, namely glucocorticoids (GCs), and generates a severe impact on the energy metabolism of the animal body. It is reported that m6A modification plays an important role in the regulation of stress response and also participates in the process of muscle growth and development. In this study, we explored the effect of GCs on the protein synthesis procession of porcine skeletal muscle cells (PSCs). We prove that dexamethasone affects the expression of SLC7A7, a main amino acid transporter for protein synthesis by affecting the level of m6A modification in PSCs. In addition, we find that SLC7A7 affects the level of PSC protein synthesis by regulating the conduction of the mTOR signaling pathway, which indicates that the reduction of SLC7A7 expression may alleviate the level of protein synthesis under stress conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Glucocorticoid Regulates the Synthesis of Porcine Muscle Protein through m6A Modified Amino Acid Transporter SLC7A7

Guo

Shi

et al. 2022

IJMS

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“…Previously, it was shown that the extract of this alga is capable of inhibition of dexamethasone-induced lipidation of LC3B-I to LC3B-II in the same model (see Section 4) [129]. The authors reported a similar effect of PYP15 on LC3B-I/II and on cathepsin L expression [128]. As no other experiments have been performed in both studies, additional data confirming the autophagy-inhibitory activity of PYP15 are needed.…”

Section: Peptidesmentioning

confidence: 93%

“…As no further investigation has been performed, this speculation awaits additional experiments. The group of Nam has examined the activity of the peptide PYP15 (DPKGKQQAIHVAPSF) isolated from the alga Pyropia yezoensis [ 127 ] using the mouse skeletal muscle atrophy model (C2C12 cells) [ 128 ]. Previously, it was shown that the extract of this alga is capable of inhibition of dexamethasone-induced lipidation of LC3B-I to LC3B-II in the same model (see Section 4 ) [ 129 ].…”

Section: Marine Compounds With a Non-validated Autophagy-modulatormentioning

confidence: 99%

Blue-Print Autophagy in 2020: A Critical Review

Dyshlovoy

2020

Marine Drugs

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Autophagy is an elegant and complex biological process that has recently attracted much attention from the scientific community. The compounds which are capable of control and modulation of this process have a promising potential as therapeutics for a number of pathological conditions, including cancer and neurodegenerative disorders. At the same time, due to the relatively young age of the field, there are still some pitfalls in the autophagy monitoring assays and interpretation of the experimental data. This critical review provides an overview of the marine natural compounds, which have been reported to affect autophagy. The time period from the beginning of 2016 to the middle of 2020 is covered. Additionally, the published data and conclusions based on the experimental results are re-analyzed with regard to the guidelines developed by Klionsky and colleagues (Autophagy. 2016; 12(1): 1–222), which are widely accepted by the autophagy research community. Remarkably and surprisingly, more than half of the compounds reported to be autophagy activators or inhibitors could not ultimately be assigned to either category. The experimental data reported for those substances could indicate both autophagy activation and inhibition, requiring further investigation. Thus, the reviewed molecules were divided into two groups: having validated and non-validated autophagy modulatory effects. This review gives an analysis of the recent updates in the field and raises an important problem of standardization in the experimental design and data interpretation.

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“…This Special Issue includes papers analyzing potential health impacts of bioactive peptides from the seaweed species Pyropia yezoensis , Chlorella pyrenoidosa , and Gracilariopsis lemaneiformis . In particular, the protective effects of the P. yezoensis peptide PYP15 against dexamethasone-induced myotube atrophy was revealed [3]. The effect of the Chlorella pyrenoidosa protein hydrolysate (CPPH) and Chlorella pyrenoidosa protein hydrolysate-calcium chelate (CPPH-Ca) on calcium absorption and gut microbiota composition was evaluated [4].…”

mentioning

confidence: 99%