Protective effect of polysaccharides from Sargassum fusiforme against UVB-induced oxidative stress in HaCaT human keratinocytes

Ji, Desheng; You, Lijun; Ren, Yinglang; Wen, Li; Zheng, Guiqing; Li, Chao

doi:10.1016/j.jff.2017.06.051

Cited by 45 publications

(33 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Polysaccharide is a component of biological organisms and an important biologically active substance that possesses multiple functions, such as antioxidant (Dammak et al, ; Ji et al, ; Ye et al, ), anti‐obesity (Chang et al, ) and immunomodulating properties (Du J et al, ; Jin et al, ; Yu et al, ). The human body lacks carbohydrate active enzymes that can utilize polysaccharides directly.…”

Section: Introductionmentioning

confidence: 99%

A novel polysaccharide isolated from Flammulina velutipes, characterization, macrophage immunomodulatory activities and its impact on gut microbiota in rats

Wang

et al. 2020

Animal Physiology Nutrition

View full text Add to dashboard Cite

The structural characteristics of a novel Flammulina velutipes polysaccharide (FVP2) were explored in this study. Besides, immunomodulatory activities of FVP2 on RAW 264.7 cell and its impact on gut microbiota in rats were investigated. FVP2 has a molecular weight of 18.3 kD, and its main components include galactose (19.96%), glucose (60.66%) and mannose (19.38%). By NMR analysis, the main‐chain structure consisted of (1 → 3)‐linked‐β‐D‐Gal, (1 → 6) ‐linked‐β‐D‐Gal, (1 → 6)‐linked‐α‐D‐Glc and (1 → 3,6)‐linked‐α‐D‐Man was identified. Results of the in vitro assays on RAW 264.7 murine macrophage cells showed FVP2 could significantly up‐regulate the expression of NO, TNF‐α and IL‐6. FVP2 was intragastrically administered to rats for 2 weeks. Compared with the control group, two caecal short‐chain fatty acids (SCFAs) concentration (isobutyric acid and butyric acid) and the abundance of beneficial microbiota of the FVP2‐treated group were significantly increased (p < .05) respectively. The results demonstrated that FVP2 could effectively enhance the level of butyric acid and increase beneficial gut microbiota, which could improve the intestinal barrier function and maintain the intestinal mucosal integrity, suggesting that FVP2 could potentially be an immunomodulators or a functional food to promote intestinal health.

show abstract

Section: Introductionmentioning

confidence: 99%

A novel polysaccharide isolated from Flammulina velutipes, characterization, macrophage immunomodulatory activities and its impact on gut microbiota in rats

Wang

et al. 2020

Animal Physiology Nutrition

View full text Add to dashboard Cite

show abstract

“…Recently, marine algaederived sulfated polysaccharides have been reported in many studies to possess cosmeceutical effects. Ji et al evaluated the UV-protective effects of a sulfated polysaccharide fraction from Sargassum fusiforme (SFP-P1) in human keratinocytes (HaCaT cells) and found that SFP-P1 reduced oxidative stress and suppressed matrix metalloproteinase (MMP) expression (Ji et al 2017 inflammation, and inhibited the expression of MMPs in UVB-irradiated mice (Kim et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced photoaging in vitro in human keratinocytes and in vivo in zebrafish

Wang

Yang

et al. 2019

Mar Life Sci Technol

View full text Add to dashboard Cite

Our previous study evaluated the in vitro and in vivo antioxidant activities of sulfated polysaccharides from a Celluclastassisted extract of Hizikia fusiforme (HFPS). The results indicate that HFPS possesses potent antioxidant activity and suggest the potential use of HFPS to combat photoaging. In this study, we investigated the ultraviolet (UV) protective effect of HFPS in vitro in keratinocytes (HaCaT cells) and in vivo in zebrafish. The results indicate that HFPS significantly reduced the level of intracellular reactive oxygen species (ROS) and improved the viability of UVB-irradiated HaCaT cells. In addition, HFPS remarkably decreased apoptosis formation in UVB-irradiated HaCaT cells in a dose-dependent manner. The in vivo test results also demonstrate that HFPS significantly reduced intracellular ROS levels, cell death, NO production, and lipid peroxidation levels in UVB-irradiated zebrafish in a dose-dependent manner. These results suggest that HFPS possesses strong in vitro and in vivo UV-protective effects, making it a potential ingredient in the cosmeceutical industry.

show abstract

“…En primer lugar, al tratarse de extractos brutos en los que no ha habido fraccionamiento, purificación o aislamiento de las diferentes fases, como en otros trabajos con diversas matrices [19,33,35,53,54], los NOAEL y las LC 50 obtenidos para cada extracto podrían verse afectados por la mortalidad causada por el pH o la concentración salina de dicho extracto. Estos efectos podrían cuantificarse realizando ensayos toxicológicos con las diferentes fracciones del extracto para detectar aquellas que muestren actividad tóxica [55].…”

Section: Discussionunclassified

“…En este sentido, la línea celular HaCaT (queratinocitos humanos inmortalizados) se ha utilizado ampliamente en ensayos de crecimiento y diferenciación celular [30], aunque se utilizan también para detectar actividad citotóxica y antitumoral en diversos compuestos y extractos. Concretamente, se ha utilizado tanto en ensayos de extractos de plantas [15,31,32] como de algas [33,34].…”

Section: Introductionunclassified

Determinación de la actividad tóxica de extractos optimizados de Palmaria y Porphyra para el tratamiento de la lesión por isquemia/reperfusión

Irisarri

Sanz

2020

Nereis

View full text Add to dashboard Cite

La lesión por isquemia-reperfusión (I/R) puede provocar rechazos en los trasplantes de órganos debido al daño a nivel celular que producen los Radicales Libres de Oxígeno (ROS). Se ha comprobado que los antioxidantes naturales pueden reducir este daño. Los extractos de muchas algas poseen una actividad antioxidante notable, pero se necesita comprobar la toxicidad de estos antes de proponerlos como fármacos. En este estudio, se realizaron dos ensayos toxicológicos con queratinocitos humanos inmortalizados (HaCaT) y nauplios de Artemia salina para valorar la toxicidad de dos extractos con actividad antioxidante de Palmaria palmata y Porphyra sp. Se obtuvieron LC50 de 7,843% (v/v) de concentración de extracto en P. palmata en el ensayo con HaCaT y 3,2583% (v/v) en el ensayo con A. salina, mientras que con Porphyra sp. se obtuvo 10,7463% (v/v) en el ensayo con HaCaT y 6,5688% (v/v) en el ensayo con A. salina. Los Niveles Sin Efecto Adverso Observable (NOAEL) obtenidos para cada alga en cada ensayo mostraron significativamente una mayor toxicidad del extracto de P. palmata que del de Porphyra sp. (p < 0,05). Finalmente, se propuso un índice que tiene en cuenta la actividad antioxidante y la toxicidad de cada extracto para predecir su eficiencia in vivo, confirmando los resultados anteriores, y por ello se escogió el extracto de Porphyra sp. como el más idóneo para continuar con los ensayos preclínicos en el desarrollo de un fármaco para el tratamiento de la lesión por I/R.

show abstract

Protective effect of polysaccharides from Sargassum fusiforme against UVB-induced oxidative stress in HaCaT human keratinocytes

Cited by 45 publications

References 40 publications

A novel polysaccharide isolated from Flammulina velutipes, characterization, macrophage immunomodulatory activities and its impact on gut microbiota in rats

A novel polysaccharide isolated from Flammulina velutipes, characterization, macrophage immunomodulatory activities and its impact on gut microbiota in rats

Protective effect of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced photoaging in vitro in human keratinocytes and in vivo in zebrafish

Determinación de la actividad tóxica de extractos optimizados de Palmaria y Porphyra para el tratamiento de la lesión por isquemia/reperfusión

Contact Info

Product

Resources

About