Protective Effect of MonoclonalAntibodies to Newcastle Disease Virus in Passive Immunization

Umino, Y.; Kohama, T.; Satô, Takeshi; Sugiura, Akira

doi:10.1099/0022-1317-71-5-1199

Cited by 32 publications

(15 citation statements)

References 20 publications

(22 reference statements)

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“…These results suggest that NDV F is a better protective antigen than NDV HN. NDV F protein has been previously shown to provide protection against NDV challenge in chickens (55,61,64,65). We speculate that the better protective ability of F antibody may be due to inhibition of virus entry into the cells.…”

Section: Discussionmentioning

confidence: 89%

Evaluation of the Newcastle Disease Virus F and HN Proteins in Protective Immunity by Using a Recombinant Avian Paramyxovirus Type 3 Vector in Chickens

Kumar

Nayak

Collins

et al. 2011

J Virol

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Newcastle disease virus (NDV) belongs to serotype 1 of the avian paramyxoviruses (APMV-1) and causes severe disease in chickens. Current live attenuated NDV vaccines are not fully satisfactory. An alternative is to use a viral vector vaccine that infects chickens but does not cause disease. APMV serotype 3 infects a wide variety of avian species but does not cause any apparent disease in chickens. In this study, we constructed a reverse-genetics system for recovery of infectious APMV-3 strain Netherlands from cloned cDNAs. Two recombinant viruses, rAPMV3-F and rAPMV3-HN, were generated expressing the NDV fusion (F) and hemagglutinin-neuraminidase (HN) proteins, respectively, from added genes. These viruses were used to immunize 2-week-old chickens by the oculonasal route in order to evaluate the contribution of each protein to the induction of NDV-specific neutralizing antibodies and protective immunity. Each virus induced high titers of NDV-specific hemagglutination inhibition and serum neutralizing antibodies, but the response to F protein was greater. Protective immunity was evaluated by challenging the immunized birds 21 days later with virulent NDV via the oculonasal, intramuscular, or intravenous route. With oculonasal or intramuscular challenge, all three recombinant viruses (rAPMV3, rAPMV3-F, and rAPMV3-HN) were protective, while all unvaccinated birds succumbed to death. These results indicated that rAPMV3 alone can provide cross-protection against NDV challenge. However, with intravenous challenge, birds immunized with rAPMV3 were not protected, whereas birds immunized with rAPMV3-F alone or in combination with rAPMV3-HN were completely protected, and birds immunized with rAPMV3-HN alone were partially protected. These results indicate that the NDV F and HN proteins are independent neutralization and protective antigens, but the contribution by F is greater. rAMPV3 represents an avirulent vaccine vector that can be used against NDV and other poultry pathogens.

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Section: Discussionmentioning

confidence: 89%

Evaluation of the Newcastle Disease Virus F and HN Proteins in Protective Immunity by Using a Recombinant Avian Paramyxovirus Type 3 Vector in Chickens

Kumar

Nayak

Collins

et al. 2011

J Virol

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“…However, while single mAbs directed to sites on the F protein have shown strong neutralizing activity, neutralization by individual HN mAbs has been shown to be incomplete, requiring antibodies to 4 sites for complete neu- tralization [14]. In passive immunization in chicks, the mAbs directed to the F protein had a protective effect against challenge with virulent NDV [32,41]. Therefore, the importance of antibodies to F protein for vaccine protection against ND has been emphasized [29], and it is important to understand the genetic diversity of the F protein gene of field NDV strains to evaluate and develop an effective vaccine against NDV.…”

Section: Discussionmentioning

confidence: 99%

“…One characteristic feature in the host immune response to paramyxovirus infection is that although antibodies to either glycoprotein can neutralize infectivity in in vitro tests, antibodies to F appear to be predominantly necessary and important for preventing infection and spread of the virus in vivo [31]. In passive immunization in chicks, monoclonal antibodies directed to 2 antigenic sites of the F protein completely suppressed virus growth and prevented the death of chickens [41]. Therefore, the F protein gene of NDV was selected to construct a candidate of the DNA vaccine or recombinant vaccine expressing this gene and proved to efficiently protect against challenge with the virulent NDV strains [22,38,39].…”

mentioning

confidence: 99%

Analysis of the Fusion Protein Gene of Newcastle Disease Viruses Isolated in Japan

Mase

Murayama

Karino

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. The complete nucleotide sequences of the fusion (F) protein gene of Newcastle disease viruses (NDV) isolated in Japan from 1930 to 2007 (45 strains total) were determined and genetically analyzed. In the deduced amino acid sequences of fusion protein, the 5 potential asparagine-linked glycosylation sites and 10 cysteine residues were all conserved in the NDV examined in this study. The major epitopes involved in virus neutralization are conserved in most of the NDV strains isolated in Japan except a few strains. By virus neutralization test, no major antigenic differences were observed among representative strains of each genotype in Japan. All chickens vaccinated with the B1 strain survived without clinical signs after challenge with 2 NDV strains isolated in Japan (velogenic strains, JP/ Ibaraki/2000 and JP/Kagoshima/91), which possess amino acids substitutions involved in virus neutralization in the F protein gene.KEY WORDS: antigenicity, B1 vaccine, fusion protein, Newcastle disease virus, phylogenetic analysis.

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“…Briefly, CEFs infected with CVI988 was transfected with a transfer plasmid, pKA4F(F), pKA4P(F), or pKA4N(F), by electroporation. At 7 days after transfection, the plaques were stained with the monoclonal antibody to NDV-F protein 313 (63,64) and goat anti-mouse immunoglobulin G (IgG) horseradish peroxidase (HRP) conjugate (Bio-Rad Laboratories, Hercules, Calif.) as described previously (47). The positive plaques were collected and plated on fresh CEFs.…”

Section: Methodsmentioning

confidence: 99%

Development of an Effective Polyvalent Vaccine against both Marek's and Newcastle Diseases Based on Recombinant Marek's Disease Virus Type 1 in Commercial Chickens with Maternal Antibodies

Sonoda

Sakaguchi

Okamura

et al. 2000

J Virol

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Protective Effect of MonoclonalAntibodies to Newcastle Disease Virus in Passive Immunization

Cited by 32 publications

References 20 publications

Evaluation of the Newcastle Disease Virus F and HN Proteins in Protective Immunity by Using a Recombinant Avian Paramyxovirus Type 3 Vector in Chickens

Evaluation of the Newcastle Disease Virus F and HN Proteins in Protective Immunity by Using a Recombinant Avian Paramyxovirus Type 3 Vector in Chickens

Analysis of the Fusion Protein Gene of Newcastle Disease Viruses Isolated in Japan

Development of an Effective Polyvalent Vaccine against both Marek's and Newcastle Diseases Based on Recombinant Marek's Disease Virus Type 1 in Commercial Chickens with Maternal Antibodies

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