Protective effect of Letinous�edodes foot peptides against ethanol‑induced liver injury in L02 cells

Ma, Lin; Huo, Chunyan; Zhang, Xiao‐Yu; Qin, Chen-Qiang; Ren, Difeng; Lu, Jun

doi:10.3892/mmr.2018.9093

Cited by 3 publications

(3 citation statements)

References 39 publications

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“…The above events eventually lead to increased ALT, AST, and ALP levels in the blood (Kazemi et al, 2017;Cengiz et al, 2019). Ma et al (2018) investigated the improving effect of lentinan on ethanol-induced L-02 cells. The findings indicated that anthocyanins had a significant impact on reducing ALT and AST activities induced by ethanol, thereby demonstrating a protective effect.…”

Section: Discussionmentioning

confidence: 99%

The “Sweat Soaking Method”-processed Radix Wikstroemia indica Extracts Attenuate Liver Toxicity and its Effect on Cytochrome P450 Protein

Wang,

Feng,

et al. 2024

Pharmacognosy Magazine

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Background: Radix Wikstroemia indica (RWI) is one of the most commonly used drugs in Miao medicine. However, RWI is characterized by high toxicity. Objectives: In this study, we aimed to observe the effect of the “sweat soaking method” processed on reducing the hepatotoxicity of RWI and on cytochrome P450 (CYP) protein expression. Materials and Methods: The study focused on investigating the impact of both RWI raw products and RWI processed products on the proliferation of L-02 cells. To assess this, a cell counting kit-8 was employed. After the administration of RWI through oral gavage for a duration of 15 days in rats, the corresponding kits were utilized to determine the serum levels of the liver index factor. Furthermore, both hematoxylin and eosin (H&E) staining and western blot analysis were conducted to analyze the liver tissues. Results: Both the raw RWI and the processed RWI at high doses inhibited the proliferation of L-02 cells, but the cell viability of the concoction group was higher than that of the raw group. The liver tissues of rats in the control and processed product groups were normal, while those in the raw product groups showed different degrees of liver damage. Additionally, compared with the control group, the activity of alkaline phosphatase (ALP), aspartate aminotransferase (AST), and alanine transaminase (ALT) in the raw product groups was notably increased in a dose-dependent manner; the three enzyme activities of the processed group were lower than those of the raw product group. In addition, the expression levels of CYP3A4, 2C19, 2C9, 1A2, and 2E1 in the liver tissue of rats in the processed group were lower than those in the raw group, while the protein expression trend of CYP2D6 was irregular. Conclusion: The detoxication mechanism of RWI, after undergoing processing, appears to be associated with reduced protein expression of CYP1A2, CYP2E1, CYP2C9, CYP2C19, and CYP3A4, ultimately leading to decreased liver injury.

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Section: Discussionmentioning

confidence: 99%

The “Sweat Soaking Method”-processed Radix Wikstroemia indica Extracts Attenuate Liver Toxicity and its Effect on Cytochrome P450 Protein

Wang,

Feng,

et al. 2024

Pharmacognosy Magazine

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“…12 For example, mushroom foot peptides could alleviate alcohol toxicity through the activation of ADH and facilitation of alcohol metabolism. 13 Additionally, peptides in the hydrolysate of crucian carp swim bladder, could restore hepatic ADH activity and subsequently enhance the alcohol metabolism capacity in mice. 14 Our previous research observed that intracellular extracts of Lactococcus lactis (L. lactis) induced by ethanol increased ADH activity and improved alcohol metabolism in mice, suggesting that BAPs presented in stress-related proteins might play a relevant role.…”

Section: Introductionmentioning

confidence: 99%

“…BAPs with beneficial health effects can be generated via in vitro or in vivo digestion, including peptides with antioxidants, anti-inflammatory, ADH-activating, and hepatoprotective effects . For example, mushroom foot peptides could alleviate alcohol toxicity through the activation of ADH and facilitation of alcohol metabolism . Additionally, peptides in the hydrolysate of crucian carp swim bladder, could restore hepatic ADH activity and subsequently enhance the alcohol metabolism capacity in mice .…”

Section: Introductionmentioning

confidence: 99%

Identification of a Novel Peptide with Alcohol Dehydrogenase Activating Ability from Ethanol-Induced Lactococcus lactis: A Combined In Silico Prediction and In Vivo Validation

Chen,

Yi,

Kang

et al. 2024

J. Agric. Food Chem.

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Alcohol dehydrogenase (ADH) is a crucial rate-limiting enzyme in alcohol metabolism. Our previous research found that ethanol-induced intracellular extracts of Lactococcus lactis (L. lactis) could enhance alcohol metabolism in mice, but the responsible compounds remain unidentified. The study aimed to screen potential ADH-activating peptides from ethanol-induced L. lactis using virtual screening and molecular docking calculation. Among them, the pentapeptide FAPEG might bind to ADH through hydrophobic interaction and hydrogen bonds, then enhancing ADH activity. Spectroscopy analysis further investigated the peptide-enzyme interaction between FAPEG and ADH, including changes in the amino acid residue microenvironment and secondary structural alterations. Furthermore, FAPEG could protect against alcoholic liver injury (ALI) in mice by reducing blood alcohol concentration, enhancing the activity of antioxidant and alcohol metabolism enzymes, and attenuating alcohol-induced hepatotoxicity, which was related to the activation of the Nrf2/keap1/HO-1 signaling pathway. The study provided preliminary evidence that the generation of ADH-activating peptides in ethanol-induced L. lactis has the potential in preventing ALI in mice using in silico prediction and in vivo validation approaches.

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Protective Effects of Several Common Amino Acids, Vitamins, Organic Acids, Flavonoids and Phenolic Acids against Hepatocyte Damage Caused by Alcohol

Wang

Zhang

Zhou

et al. 2022

Foods

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With the increase in alcohol consumption, more and more people are suffering from alcoholic liver disease (ALD). Therefore, it is necessary to elaborate the pathogenesis of ALD from the aspects of alcohol metabolism and harm. In this study, we established an alcoholic liver injury model in vitro by inducing L02 cells with different concentration of ethanol and acetaldehyde. Results showed that the metabolism of ethanol can promote the content of ROS, MDA, TNF-α, IL-6, and caspase 3, causing oxidative and inflammatory stress and membrane permeability changes. However, unmetabolized ethanol and acetaldehyde had little effect on cell membrane permeability and inflammation, indicating that ethanol metabolites were the main reason for cell membrane damage. We also evaluated the effects of amino acids (taurine and methionine), vitamins (E and vitamin D), organic acids (malic acid and citric acid), flavonoids (rutin and quercetin), and phenolic acids (ferulic acid and chlorogenic acid) on alcohol-induced cell membrane damage of L02 cells. Chlorogenic acid, taurine, vitamin E, and citric acid had remarkable effects on improving cell membrane damage. Malic acid, rutin, quercetin, and ferulic acid had obvious therapeutic effects, while vitamin D and methionine had poor therapeutic effects. The relationship between the structure and effect of active ingredients can be further studied to reveal the mechanism of action, and monomers can be combined to explore whether there is a synergistic effect between functional components, in order to provide a certain theoretical basis for the actual study of liver protection.

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Protective effect of Letinous�edodes foot peptides against ethanol‑induced liver injury in L02 cells

Cited by 3 publications

References 39 publications

The “Sweat Soaking Method”-processed Radix Wikstroemia indica Extracts Attenuate Liver Toxicity and its Effect on Cytochrome P450 Protein

The “Sweat Soaking Method”-processed Radix Wikstroemia indica Extracts Attenuate Liver Toxicity and its Effect on Cytochrome P450 Protein

Identification of a Novel Peptide with Alcohol Dehydrogenase Activating Ability from Ethanol-Induced Lactococcus lactis: A Combined In Silico Prediction and In Vivo Validation

Protective Effects of Several Common Amino Acids, Vitamins, Organic Acids, Flavonoids and Phenolic Acids against Hepatocyte Damage Caused by Alcohol

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