Protective Effect of Ischemic Preconditioning on Cold Preservation and Reperfusion Injury Associated With Rat Intestinal Transplantation

Solà, Anna; de, Javier; Gonzalez, R.; Prats, Neus; Roselló‐Catafau, Joan; Gelpí, E.; Jaurrieta, Eduardo; Hotter, Georgina

doi:10.1097/00000658-200107000-00015

Cited by 72 publications

(58 citation statements)

References 27 publications

(13 reference statements)

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“…Serum LDH levels, which is used as a marker for intestinal injury [6] , reflected intestinal damage in IR group, whereas PDTC decreased these levels. IR injury resulted in villous and crypt damage, which attributed to hemorrhage and necrosis, whereas PDTC ameliorated this effect.…”

Section: Discussionmentioning

confidence: 99%

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Pyrrolidine dithiocarbamate reduces ischemia-reperfusion injury of the small intestine

Mallick

Yang²,

Winslet³

et al. 2005

WJG

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AIM:To evaluate whether pyrrolidine dithiocarbamate (PDTC), an enhancer of HO production, attenuates intestinal IR injury.METHODS: E i g h t e e n m a l e ra t s w e r e ra n d o m l y allocated into three groups: (a) sham; (b) IR, consisting of 30 min of intestinal ischemia, followed by 2-h period of reperfusion; and (c) PDTC treatment before IR. Intestinal microvascular perfusion (IMP) was monitored continuously by laser Doppler flowmetry. At the end of the reperfusion, serum samples for lactate dehydrogenase (LDH) levels and biopsies of ileum were obtained. HO activity in the ileum was assessed at the end of the reperfusion period. RESULTS:At the end of the reperfusion in the IR group, IMP recovered partially to 42.5% of baseline (P <0.05 vs sham), whereas PDTC improved IMP to 67.3% of baseline (P <0.01 vs IR). There was a twofold increase in HO activity in PDTC group (2 062.66±106.11) as compared to IR (842.3±85.12) (P <0.001). LDH was significantly reduced (P<0.001) in PDTC group (585.6±102.4) as compared to IR group (1 973.8±306.5). Histological e x a m i n a t i o n s h o w e d t h a t t h e i l e a l m u c o s a wa s significantly less injured in PDTC group as compared with IR group. CONCLUSION:

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Section: Discussionmentioning

confidence: 99%

“…The attenuation of IR injury is vital for intestinal graft function and survival after small bowel transplantation [6] . IR injury is associated with the breakdown of microvascular perfusion with subsequent impairment of tissue oxygenation [7] .…”

Section: Introductionmentioning

confidence: 99%

Pyrrolidine dithiocarbamate reduces ischemia-reperfusion injury of the small intestine

Mallick

Yang²,

Winslet³

et al. 2005

WJG

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“…The exact mechanism by which IPC confers protection in the intestine is unclear. Several mediators have been advocated to play a crucial role in this protective phenomenon, including adenosine, 22 nitric oxide, 23 oxidative stress, 24 heme oxygenase one, 25 and antiapoptosis. 26 Cytokines are known to exert their effects in distant organs; therefore, their local increase follo wing IRI is the beginning for systemic IRI.…”

Section: Discussionmentioning

confidence: 99%

Untitled

Vrakas

Tsalis²,

Roidos³

et al. 2017

Exp Clin Transplant

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Objectives: Our study aimed to determine whether antithrombin plays a synergistic role in accentuating the effects of intestinal ischemic preconditioning. Materials and Methods: Fifty rats were randomly allocated to 5 groups (10 rats/group) as follows: sham treatment (group 1); ischemia-reperfusion (group 2); ischemic preconditioning followed by ischemiareperfusion (group 3); antithrombin + ischemia-reperfusion, similar to group 2 but including antithrombin administration (group 4); and antithrombin + ischemic preconditioning, similar to group 3 but including antithrombin administration (group 5). Blood samples and liver specimens were obtained for measurement of cytokines, myeloperoxidase, and malondialdehyde. Liver biopsies were examined by electron microscopy. Results: Intestinal ischemia-reperfusion induced a remote hepatic inflammatory response as evidenced by the striking increase of proinflammatory cytokines, myeloperoxidase, and malondialdehyde. Tumor necro sis factor-α levels in group 5 (12.48 ± 0.7 pg/mL) were significantly lower than in group 3 (13.64 ± 0.78 pg/mL; P = .014). Mean interleukin 1β was lower in group 5 (9.52 ± 0.67pg/mL) than in group 3 (11.05 ± 1.9 pg/mL; P > .99). Mean interleukin 6 was also significantly lower in group 5 (17.13 ± 0.54 pg/mL) than in group 3 (23.82 ± 1 pg/mL; P < .001). Myeloperoxidase levels were significantly higher in group 3 (20.52 ± 2.26 U/g) than in group 5 (18.59 ± 1.03 U/g; P = .025). However, malondialdehyde levels did not significantly improve in group 5 (4.55 ± 0.46 μmol) versus group 3 (5.17 ± 0.61 μmol; P = .286). Tumor necrosis factor-α, interleukin 6, and myeloperoxidase findings show that antithrombin administration further attenuated the inflammatory response caused by ischemia-reperfusion, suggesting a synergistic effect with ischemic preconditioning. These findings were confirmed by electron microscopy. Conclusions: The addition of antithrombin to ischemic preconditioning may act to attenuate or prevent damage from ischemia-reperfusion injury by inhi biting the release of cytokines and neutrophil infiltration.

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“…Bağırsaklar: İskemik önkoşullama uygulaması bağırsaklarda da iskemi-reperfüzyon sonrası doku nekrozunun gelişmesini geciktirerek, bakteriyel translokasyonu azaltarak, intestinal mikrovasküler perfüzyonu ve oksijenizasyonu artırarak olumlu etkilerini göstermiştir (62) . Sıçan donör bağırsağına uygulanan İÖ sonucunda bağırsağın, korunduğu solüsyona daha az miktarda laktat dehidrojenaz saldığı ve reperfüzyon sonrasında daha az inflamasyona ve histolojik hasara uğradığı saptanmışsa da, bu konuda insanlar üzerinde yapılmış bir çalışma yoktur (63) .…”

Section: Karaciğerunclassified

Clinical Importance of Ischemic Preconditioning

Oncel¹,

Dinçer²,

Cinel³

2012

GKDA Derg

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Protective Effect of Ischemic Preconditioning on Cold Preservation and Reperfusion Injury Associated With Rat Intestinal Transplantation

Abstract: Preconditioning protects the intestinal grafts from cold preservation and reperfusion injury in the rat intestinal transplantation model. Nitric oxide is involved in this protection.

Cited by 72 publications

References 27 publications

Pyrrolidine dithiocarbamate reduces ischemia-reperfusion injury of the small intestine

Pyrrolidine dithiocarbamate reduces ischemia-reperfusion injury of the small intestine

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Clinical Importance of Ischemic Preconditioning

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