Pyrrolidine dithiocarbamate reduces ischemia-reperfusion injury of the small intestine

Abstract

AIM:To evaluate whether pyrrolidine dithiocarbamate (PDTC), an enhancer of HO production, attenuates intestinal IR injury.METHODS: E i g h t e e n m a l e ra t s w e r e ra n d o m l y allocated into three groups: (a) sham; (b) IR, consisting of 30 min of intestinal ischemia, followed by 2-h period of reperfusion; and (c) PDTC treatment before IR. Intestinal microvascular perfusion (IMP) was monitored continuously by laser Doppler flowmetry. At the end of the reperfusion, serum samples for lactate dehydrogenase (LDH) levels and biopsies of ileum were obtained. HO activity in the ileum was assessed at the end of the reperfusion period. RESULTS:At the end of the reperfusion in the IR group, IMP recovered partially to 42.5% of baseline (P <0.05 vs sham), whereas PDTC improved IMP to 67.3% of baseline (P <0.01 vs IR). There was a twofold increase in HO activity in PDTC group (2 062.66±106.11) as compared to IR (842.3±85.12) (P <0.001). LDH was significantly reduced (P<0.001) in PDTC group (585.6±102.4) as compared to IR group (1 973.8±306.5). Histological e x a m i n a t i o n s h o w e d t h a t t h e i l e a l m u c o s a wa s significantly less injured in PDTC group as compared with IR group. CONCLUSION:

Protective Effects of Ascorbic Acid Pretreatment in a Rat Model of Intestinal Ischemia-Reperfusion Injury: A Histomorphometric Study

“…Support for this theory is provided by the observation that ischemic injury to the small bowel is most severe at the tip and is attenuated by intraluminal perfusion with oxygenated saline 26 . A variety of associations between ascorbic acid and IR injury has been described recently [27][28][29][30][31] . Cizova et al 27 demonstrated that the ascorbic and uric acid concentrations increased generally after IR in comparison to intact controls, but this increase was not sufficient to prevent lipid peroxidation in serum and intestinal mucosa.…”

Vascular Pathology of Ischemia/Reperfusion Injury of Rat Small Intestine

“…The development of injuries in early reperfusion was studied using 60-min ischemia and 120-min reperfusion periods. These terms are not lethal to animals but cause damage to the organ [Mallick et al, 2005].…”

Evaluation of the effect of pyrrolidine dithiocarbamate in suppressing inflammation in mice with dextran sodium sulfate-induced colitis

“…NF-κB p65 protein has been reported to exhibit increased expression in nuclear extracts from local mucosal tissue specimens of patients with inflammatory bowel disease [10,11] . Recently reports have been published to evaluate the inflammation suppressing effect of antisense oligonucleotide for NF-κB (p65) administered to mice with TNBS-induced enteritis (a model of Crohn's disease) [12] and mice with DSS-induced colitis (a model of ulcerative colitis) [13] or ischemia-reperfusion injury of the small intestine [14] . It has been reported that NF-κB binds to the DNA sequence termed "κB sequence" and induces expression of the genes regulated by this sequence.…”