Protective effect of individual glycoproteins of Newcastle disease virus expressed in insect cells: the fusion protein derived from an avirulent strain had lower protective efficacy

Kamiya, Naohiro; Masahiro, Niikura; Ono, M.; Cui, Kai; Matsuura, Yoshiharu; Mikami, Taisei

doi:10.1016/0168-1702(94)90085-x

Cited by 20 publications

(10 citation statements)

References 16 publications

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“…Mori et al [19] reported that a recombinant baculovirus expressing the F protein of NDV D26 induced a protective effect. In contrast, Kamiya et al [8] reported that the chickens immunized with rNDF or rNDHN proteins of the velogenic Miyadera strain (rMF or rMHN), the rNDHN protein of the D26 strain (rDHN), or a equal mixture of rMF and rMHN were completely protected from a subsequent challenge. However, chickens immunized with the rNDF protein of the lentogenic D26 strain (rDF) showed a lower protective efficacy.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the combined inoculation of the F and HN glycoproteins had a synergistic effect against a subsequent NDV challenge. Kamiya et al [8] reported that chickens immunized with a mixture of rMF and rMHN developed a similar level of specific antibodies and protective efficacy compared with the chickens immunized with the individual glycoproteins. In this study, a synergistic effect of the combined inoculation of the F and HN glycoproteins was not detected after the second immunization, as reported by Kamiya et al [8], even though it could be recognized after the first inoculation, in which the individual glycoproteins rarely induced a protective effect.…”

Section: Discussionmentioning

confidence: 99%

“…Kamiya et al [8] reported that chickens immunized with a mixture of rMF and rMHN developed a similar level of specific antibodies and protective efficacy compared with the chickens immunized with the individual glycoproteins. In this study, a synergistic effect of the combined inoculation of the F and HN glycoproteins was not detected after the second immunization, as reported by Kamiya et al [8], even though it could be recognized after the first inoculation, in which the individual glycoproteins rarely induced a protective effect. Also, a single inoculation of the combined recombinant glycoprotein was sufficient to induce a protective effect, whereas the individual rND protein needed a booster immunization to induce protection.…”

Section: Discussionmentioning

confidence: 99%

“…The viral genome encodes six proteins from the 5' terminus to the 3' terminus: RNA-directed RNA polymerase (L gene), hemagglutinin-neuraminidase (HN gene), fusion (F gene), matrix (M gene), phosphoprotein (P gene), and nucleocapsid (NP) protein [2]. The HN and F glycoprotein on the surface of NDV are important for virus infectivity and pathogenicity, and either of these proteins can induce protective immunity [8,15,20,21,24,30]. …”

Section: Introductionmentioning

confidence: 99%

“…Kamiya et al [8] also reported the protective effects and antibody response of rND F and rND HN derived from the virulent and avirulent NDV strains. They used the D26 and Miyadera strains as avirulent and virulent strains, respectively, to evaluate the protective effects and the rND F glycoprotein derived from the avirulent strain had a lower protective effect.…”

Section: Introductionmentioning

confidence: 99%

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Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin-neuraminidase proteins

et al. 2008

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Recombinant baculoviruses containing the fusion (F) and hemagglutinin-neuraminidase (HN) glycoprotein gene of the viscerotropic velogenic (vv) Newcastle disease virus (NDV) isolate, Kr-005/00, and a lentogenic La Sota strain of the NDV were constructed in an attempt to develop an effective subunit vaccine to the recent epizootic vvNDV. The level of protection was determined by evaluating the clinical signs, mortality, and virus shedding from the oropharynx and cloaca of chickens after a challenge with vvNDV Kr-005/00. The recombinant ND F (rND F) and recombinant HN (rND HN) glycoproteins derived from the velogenic strain provided good protection against the clinical signs and mortality, showing a 0.00 PI value and 100% protection after a booster immunization. On the other hand, the combined rND F + HN glycoprotein derived from the velogenic strain induced complete protection (0.00 PI value and 100% protection) and significantly reduced the amount of virus shedding even after a single immunization. The rND F and rND HN glycoproteins derived from the velogenic strain had a slightly, but not significantly, greater protective effect than the lentogenic strain. These results suggest that the combined rND F + HN glycoprotein derived from vvNDV can be an ideal subunit marker vaccine candidate in chickens in a future ND eradication program.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin-neuraminidase proteins

et al. 2008

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Expression of the Newcastle disease virus fusion protein in transgenic maize and immunological studies

et al. 2006

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Transgenic plants have been employed successfully as a low-cost system for the production of therapeutically valuable proteins, including antibodies, antigens and hormones. Here, we report the expression of the fusion (F) gene of the Newcastle disease virus (NDV) in transgenic maize plants. The expression of the transgene, driven by the maize ubiquitin promoter, caused accumulation of the F protein in maize kernels. The presence of the transgene was verified by Southern and western blots. Feeding chickens with kernels containing the F protein induced the production of antibodies, which conferred protection against a viral challenge. This protection was comparable to that conferred by a commercial vaccine. Possible uses of this plant-based F protein as a potential mucosal vaccine are discussed.

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Evaluation of a fusion gene-based DNA prime-protein boost vaccination strategy against Newcastle disease virus

Khulape

Maity

Pathak

et al. 2019

Trop Anim Health Prod

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Protective effect of individual glycoproteins of Newcastle disease virus expressed in insect cells: the fusion protein derived from an avirulent strain had lower protective efficacy

Cited by 20 publications

References 16 publications

Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin-neuraminidase proteins

Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin-neuraminidase proteins

Expression of the Newcastle disease virus fusion protein in transgenic maize and immunological studies

Evaluation of a fusion gene-based DNA prime-protein boost vaccination strategy against Newcastle disease virus

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