Evaluation of a fusion gene-based DNA prime-protein boost vaccination strategy against Newcastle disease virus

Khulape, Sagar A.; Maity, Hemanta Kumar; Pathak, D. C.; Narayan, Raj; Ramakrishnan, Saravanan; Chellappa, Madhan Mohan; Dey, Sohini

doi:10.1007/s11250-019-01967-2

Cited by 6 publications

(5 citation statements)

References 34 publications

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“…Other delivery methods, including the use of liposomes, nano-chitosan or Salmonella spp as a vector, have been used to improve NDV DNA vaccines [7,11]. A DNA prime-protein booster strategy can further increase the protection rate [14]. A DNA vaccine nano-encapsulated with hollow Ag@ SiO 2 nanoparticles stimulates strong mucosal immunity when administered intranasally and has been shown to be protective against NDV.…”

Section: Discussionmentioning

confidence: 99%

Immune effect of a Newcastle disease virus DNA vaccine with IL-12 as a molecular adjuvant delivered by electroporation

Xie

et al. 2020

Arch Virol

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Newcastle disease (ND), caused by virulent Newcastle disease virus (NDV) strains, has been one of the most problematic diseases affecting the poultry industry worldwide. Conventional vaccines provide effective protection for birds to survive ND outbreaks, but they may not completely suppress NDV shedding. NDV strains circulate on farms for a long time after the initial infection and cause potential risks. A new vaccine with fast clearance ability and low viral shedding is needed. In this study, we used interleukin-12 (IL-12) as an adjuvant and electroporation (EP) as an advanced delivery system to improve a DNA vaccine candidate. The fusion (F) protein gene from an NDV strain of the prevalent genotype VII.1.1 was cloned to prepare the vaccine. Chickens immunized with the F gene DNA vaccine co-delivered with an IL-12-expressing plasmid DNA showed higher neutralizing antibody levels and stronger concanavalin-A-induced lymphocyte proliferation than those treated with the F gene DNA vaccine alone. The co-delivered vaccine provided 100% protection, and less viral shedding and a shorter release time were observed in challenged chickens than when the F gene DNA vaccine was administered alone. The use of F gene DNA combined with IL-12 delivered by electroporation is a promising approach for vaccination against ND. Abbreviations APC Antigen-presenting cell CEF Chicken embryo fibroblast chIL-12 Chicken IL-12 DMEM Dulbecco's modified Eagle'

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Section: Discussionmentioning

confidence: 99%

Immune effect of a Newcastle disease virus DNA vaccine with IL-12 as a molecular adjuvant delivered by electroporation

Xie

et al. 2020

Arch Virol

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“…In recent years, F gene-based DNA prime protein vaccine strategy has been experienced and its results showed that DNA prime protein boost technique can easily boost up the immune response in poultry birds. This approach gave 91.6% protection from NDV and showed higher immunity level than DNA vaccines (Khulape et al 2019). These are safer alternatives against ND vaccination challenges and more capable of inducing both humoral and cell mediated (CD4+ and CD8+) immune responses.…”

Section: Dna Vaccinesmentioning

confidence: 98%

Recent Trends in Immunology and Vaccination Against Newcastle Disease Virus

2021

Int J Vet Sci

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“…A subunit vaccine based on the NDV F protein was developed by expressing the F protein in Pichia pastoris, this vaccine candidate adjuvanted with flagellin (FliC) stimulated both humoral and cellular immune response against the challenge strain [165]. Moreover, recent findings suggest DNA prime-protein boost vaccination approach applying the full-length NDV F gene to provide an enhanced immune response against NDV in poultry [166]. A transgenic rice ND subunit vaccine reportedly elicited neutralizing antibodies against both homologous and heterologous NDV strains [167].…”

Section: Recent Advances In Nd Vaccine Developmentmentioning

confidence: 99%

Towards Improved Use of Vaccination in the Control of Infectious Bronchitis and Newcastle Disease in Poultry: Understanding the Immunological Mechanisms

et al. 2021

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Infectious bronchitis (IB) and Newcastle disease (ND) are two important diseases of poultry and have remained a threat to the development of the poultry industry in many parts of the world. The immunology of avian has been well studied and numerous vaccines have been developed against the two viruses. Most of these vaccines are either inactivated vaccines or live attenuated vaccines. Inactivated vaccines induce weak cellular immune responses and require priming with live or other types of vaccines. Advanced technology has been used to produce several types of vaccines that can initiate prime immune responses. However, as a result of rapid genetic variations, the control of these two viral infections through vaccination has remained a challenge. Using various strategies such as combination of live attenuated and inactivated vaccines, development of IB/ND vaccines, use of DNA vaccines and transgenic plant vaccines, the problem is being surmounted. It is hoped that with increasing understanding of the immunological mechanisms in birds that are used in fighting these viruses, a more successful control of the diseases will be achieved. This will go a long way in contributing to global food security and the economic development of many developing countries, given the role of poultry in the attainment of these goals.

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Evaluation of a fusion gene-based DNA prime-protein boost vaccination strategy against Newcastle disease virus

Cited by 6 publications

References 34 publications

Immune effect of a Newcastle disease virus DNA vaccine with IL-12 as a molecular adjuvant delivered by electroporation

Immune effect of a Newcastle disease virus DNA vaccine with IL-12 as a molecular adjuvant delivered by electroporation

Recent Trends in Immunology and Vaccination Against Newcastle Disease Virus

Towards Improved Use of Vaccination in the Control of Infectious Bronchitis and Newcastle Disease in Poultry: Understanding the Immunological Mechanisms

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