Protective effect ofPaeoniae radix albaroot extract on immune alterations in mice with atopic dermatitis

Abstract

Atopic dermatitis is a progressive inflammatory disease characterized by type 2 helper T cell (T2) reactivity. The aim of this study was to examine the therapeutic effects of Paeoniae radix alba root extract using a murine model of atopic dermatitis. Atopic dermatitis was induced in a murine model characterized by immune alterations skewed toward T2 reactivity and pathophysiological dermal alterations which resemble human atopic dermatitis. The root extract at 1% or 6% was applied to the mouse dorsal skin for 3 weeks following induction of atopic dermatitis. Splenocytes were stimulated with immobilized anti-CD3 for 48 h to measure cytokine production. Levels of serum IgE, IgG1, and IgG2a were quantitated. Epidermal thickness and numbers of skin mast cells were determined. Mice in which atopic dermatitis was induced displayed increased numbers of skin mast cells, increased frequency of scratching, elevated serum IgE levels, increased ratios of IgG1 to IgG2a, and ratios of IL-4 to IFN-γ. The frequency of scratching was significantly decreased following application of 1% or 6% extract for 1 week. The root extract also reversed T2 skewing, as serum IgE levels, ratio of serum IgG1 to IgG2a, and ratio of IL-4 to IFN-γ production by in vitro-stimulated splenocytes were all suppressed following application of 1% or 6% extract for 3 weeks. Taken together Paeoniae radix alba root extract is suggested to reverse the immunological alterations and skin manifestations symptoms found in atopic dermatitis.

Modulation of the Gut Microbiota by Sihocheonggan-Tang Shapes the Immune Responses of Atopic Dermatitis

“…In this study, we found that SHCGT suppressed the increased Th2 and Th1 immune responses in mice with AD and that SCFAs also directly reduced the expression of IL-6 and ICAM-1 in TNF-α/INF-γ-induced keratinocytes. SHCGT contains several compounds that are known to attenuate AD, including berberine and paeoniflorin (Tsang et al, 2016;Jo et al, 2018), and was also found to decrease the expression of IL-6 in an in vitro model of AD in our study. However, the actual concentrations of these active compounds in SHCGT may be extremely low in the circulation after oral administration.…”

Neuroprotective and Anti-Inflammatory Effects of Pinus densiflora Bark Extract in Gerbil Hippocampus Following Transient Forebrain Ischemia

“…Extracts from natural sources contain multiple biological-active compounds and have gained increasing attention due to their diverse pharmacological actions [ 17 , 18 , 19 ]. For this reason, they have been developed as commercial products for improving human health [ 20 ].…”

<p>Research Progress on Classical Traditional Chinese Medicine Jieyu Pills in the Treatment of Depression</p>

“…It is commonly used for blood deficiency, irregular menstruation, spontaneous sweating, night sweats, flank pain, abdominal pain, limb pain, headache, and dizziness. 6,7 Previous studies showed that PRA could be used for anti-inflammatory effects, 8 immune regulation, 9 treatment of depression 10,11 and anemia, 12 promotion of peripheral nerve regeneration, 13 and treatment of viral infections. 14 Modern studies of pharmacological mechanisms have found that the main active components of PRA include paeoniflorin, paeonol, paeonin, benzoylpaeoniflorin, albiflorin, oxypaeoniflorin, paeoniflorigenone, benzoic acid, β-sitosterol, and gal-lotannin.…”