Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat

Abstract

Background: The study was aimed to investigate the protective effect of Asarum sieboldii Miq. essential oil (AEO) on ovalbumin (OVA)-induced allergic rhinitis (AR) in rats. Methods and results: Sixty Sprague–Dawley male rats were randomly divided into six groups (n=10): control, model, cetirizine (Cet, 4.65 g/kg), and AEO (0.5, 1.5, 3 g/kg) groups. All animals except the control group received repeated intranasal instillation with 20 μl of 20% OVA in Al(OH)3 saline solvent for 15 days. The control group was intranasally instilled with 5 mg/ml of Al(OH)3 instead of the same procedure. In the 15 days, Cet and AEO were orally administrated for 28 days. At the end of the drug administration, 20 μl of 5% OVA was given to animals to stimulate allergic reaction, then the rat behavioral detection, assessment of the patho-morphological changes in nasal mucosa, and the serum biomarkers were determined. The result showed that AEO could significantly reduce the amount of nasal secretions, sneezing, and the degree of nasal scratching in AR rats with EC50 = 1.5 and 2.8 g/kg, respectively. The degree of nasal mucosal inflammation in AEO group improved, the levels of immunoglobulin E (IgE), histamine, IL-4, IL-5, IL-17 were decreased, and the level of IFN-γ was increased obviously with EC50 = 2 g/kg. Conclusion: The study suggested that the possible mechanism might be related with the inhibition of histamine release and regulation of the cytokine levels, which plays an important role in the treatment of AR.

Yiqi Jiemin decoction alleviates allergic rhinitis in a guinea pig model by suppressing inflammation, restoring Th1/Th2 balance, and improving cellular metabolism

“…Traditional Chinese Medicines (TCMs) have been used for many centuries to treat multi-factorial diseases including AR. YJD is a widely used TCM regime to treat inflammatory diseases in China [ 16 , 18 , 19 , 24 ]. In this study, we confirmed that YJD alleviated OVA-induced AR in the guinea pig model by suppressing inflammation and improving cellular metabolism.…”

“…The AR model guinea pigs were established as described previously with minor modifications [ 18 , 19 ]. In brief, guinea pigs were sensitized for 15 consecutive days with intraperitoneal injections of 750 μL OVA solution, which consisted of 2 mg ovalbumin (Sigma-Aldrich, USA; cat.…”

“…Traditional Chinese medicine (TCM) has been widely accepted and practiced in China for more than two thousand years [ 16 , 17 ]. Previous studies have shown that acupoint herbal patching alone or in combination with western medicine is more effective in AR patients than treatment with placebo or western medicine alone [ 18 , 19 ]. Yan et al found that intranasal acupuncture combined with Yiqi Jiemin Decoction significantly attenuated the symptom of moderate-severe allergic rhinitis with deficiency of lung and spleen qi [ 20 ].…”

Immunomodulative Effects of Chamaecyparis obtusa Essential Oil in Mouse Model of Allergic Rhinitis

“…Inflammatory cell infiltration was downregulated by EOCO, and the increased eosinophil count was significantly suppressed through intranasal instillation of triamcinolone (68.6%) and EOCO (33.0% for 0.01% EOCO and 43.2% for 0.1% EOCO). Pro-inflammatory cytokines, including IL-6 and IL-8, have been known as major cytokines involved in mucus hypersecretion and AR pathogenesis [ 18 ]. Intranasal EOCO instillation suppressed mucus-producing cells in the sinonasal mucosa.…”

Pharmacokinetic Study of Safrole and Methyl Eugenol after Oral Administration of the Essential Oil Extracts of Asarum in Rats by GC-MS

“…It has attracted wide attention from researchers because of its high content and strong activities. Modern pharmacological studies have demonstrated that various volatile oil ingredients in Asarum have a lot of activities such as antibacterial, treatment of allergic rhinitis, antinociceptive, anti-inflammatory, and antiallergic [5][6][7][8][9]. On the other hand, toxicological researchers found that the volatile oil ingredients in Asarum have strong phytotoxic activity and it was toxic to D. farinae adults [10,11].…”