Protective effect of ginsenoside Rg1 on MPP + -induced apoptosis in SHSY5Y cells

Abstract

The neuroprotective mechanism of Rg1 was studied in this paper by means of its obvious anti-apoptotic effect on human SHSY5Y cells. SHSY5Y cells were treated with MPP+ (1-methyl-4-phenyl-pyridinium) for 72 hours to induce apoptosis. During the apoptosis, production of reactive oxygen species (ROS), activation of c-Jun N-terminal kinase (JNK) and activation of caspase-3 were observed. The results showed that the signal transduction pathway of MPP+-induced apoptosis might be ROS to JNK, then to caspase-3. MPP+-induced apoptosis in SHSY5Y cells was obviously inhibited in both NAC (N-acetylcysteine) pretreated groups and Rg1 pretreated groups. Meanwhile, compared to that of the controls, our results showed decreased level of ROS, less JNK activity and lower expression of cleaved caspase-3 in pretreated NAC groups and in Rg1 pretreated groups. The protection by Rg1 might be mediated by removing of ROS. The removal of ROS might inhibit the activity of JNK and the expression of cleaved caspase-3. These results suggest that ginsenoside Rg1 may take effect through its anti-apoptotic activity in neurodegenerative diseases.

Effect of the SOD mimetic MnL4 on in vitro and in vivo oxaliplatin toxicity: Possible aid in chemotherapy induced neuropathy

“…In astrocytes, the concomitant incubation with MnL4 reduces it, whereas in SH-SY5Y, MnL4 is ineffective. An anti-apoptotic effect of antioxidants has been described in SH-SY5Y treated with noxious stimuli like MPP þ [36], 1,2-diacetylbenzene [37] or hydrogen peroxide [38]. In those reports, an exogenous oxidant stimulus has been employed; on the contrary, as already described [20], oxaliplatin is not a direct oxidant molecule.…”

The water extract of Omija protects H9c2 cardiomyoblast cells from hydrogen peroxide through prevention of mitochondrial dysfunction and activation of caspases pathway

“…The study focused on discovering antioxidant components from the traditional Oriental medicine as a model for antioxidant-based composite therapy for heart disorders associated with oxidative stresses. Of note, the ginsenosides of ginseng in the constituent herb of Omija are known to have neuroprotective effects, including an improvement of hippocampal neuronal function, and the prevention of apoptotic death of cortical neurons and cultured PC12 cells (Chen et al, 2001). In traditional Oriental medicine, multi-component prescriptions are usually preferred in the treatment of various diseases.…”

Immuno-regulatory Effects of CKBM on the Activities of Mitogen-Activated Protein Kinases and the Release of Cytokines in THP-1 Monocytic Cells

“…8) Fructus crataegi contains chlorogenic acid which is capable of triggering p38 activation in chronic myelogenous leukemia cells; 21) while betulinic acid isolated from Ziziphus jujube also shows a stimulatory effect on p38 in human melanoma cells. 22) All these findings suggest that the differential regulation of MAPK described in this report is likely to be the integrated effects of various chemical components in CKBM, resulting in a persistent activation of p38, an inhibitory effect on JNK and a lack of ERK regulation in THP-1 cells (Fig.…”