Protective effect of gallic acid on apoptosis of sperm and in vitro fertilization in adult male mice treated with cyclophosphamide

Abstract

Background Alteration of free radicals (reactive oxygen species) causes mammals' sperm damage. Gallic acid (GA) is known as an antioxidant which is effective against oxidative stress. The purpose of this study was to evaluate the antioxidant effects of GA on the sperm apoptosis and in vitro fertilization (IVF) in adult male mice treated with cyclophosphamide (CP). Materials and Methods Following a pilot study to find the dose responses of GA, 40 adult male naval medical research institute (NMRI) mice (32 ± 3 g) were divided into five groups (n = 8): control, sham (normal saline, NS: 0.2 mL per day), CP (15 mg kg–1 per week; intraperitoneal, IP), GA (12.5 mg kg –1 per day; IP), and GA+CP. After the treatment, sperm parameters were analyzed. The apoptosis of sperm was measured by Annexin‐PI staining method followed by flow cytometry detection. Fertility was assessed by IVF method among the groups. Results The difference in sperm parameter and fertility rate between the control (% 80.05 ± 6.53) and cyclophosphomide groups (% 51.82 ± 10.78) was significant (P < .001) but GA plus CP (% 78.16 ± 5.71) restored the fertilization rate (P < .001). Also, a remarkable increase was noted regarding apoptotic sperm in CP group vs the control group. The comparison in the five groups shows that GA cotreatment was significantly effective in reducing the apoptosis rate caused by cyclophosphamide (P < .05). Conclusion It was ultimately attained that GA has a potent antioxidant effect which could inhibit the detrimental effect of CP on the apoptosis and fertility rate of sperm in the mouse.

Effects of Carnosic and Gallic Acid on Ram Sperm Parameters and Seminal Plasma Homocysteine-Nesfatin Levels after Thawing

“…Similar to the study presented, Güngör et al ( 28) determined 2 mM GA dose added to the ram sperm diluent had a positive effect on membrane integrity rates. GA has a powerful antioxidant effect that can prevent the harmful effect of CP on the apoptosis of Mouse sperm (29). In our study it was determined that GA 2mM had a protective effect on plasma membrane integrity and plasma membrane functionality.…”

Uncovering the mechanism of the effects of Paeoniae Radix Alba on iron-deficiency anaemia through a network pharmacology-based strategy

“…Component-target networks of PRA Table 1). The literature shows that albiflorin and paeoniflorin are the active ingredients of PRA responsible for its ameliorative effects on anaemia [20], and gallic acid has anti-inflammatory, antioxidant and antitumour effects [43]. Therefore, these chemical components were also considered candidate components, and their structures were verified with SciFinder and ChemSpider.…”

Influence of Dried Egg Yolk with or without Taurine in Extender on Sperm Characteristic of Cryopreserved Buffalo Semen

“…The insignificant increase in sperm motility in post-thawed semen agreed with several authors (Bucak et al, 2007;Atessahin et al, 2008), whom concluded an expansion of sperm motility after thawing in various animals. The noticed improvement in quality of cryopreserved semen by addition of taurine, especially membrane integrity may be attributed to that taurine has a protecting influence alongside lipid peroxidation, behind motility, optimistic properties on viability and membrane endurance of sperm after thawing (Chen et al, 1993;Sanchez et al, 1997;Pena et al, 1998;Mehraban et al, 2019).…”